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Preparation method of vitamin K2

A vitamin and acetone technology, applied in the field of vitamin K2 preparation, can solve the problems of low extraction rate, high cost, unsatisfactory extraction effect, etc., and achieve the effect of improving purity and avoiding waste

Active Publication Date: 2021-09-17
SHANDONG RUNDE BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods either have unsatisfactory extraction effects, such as low extraction rate and low purity, or the process is complex and costly, and the production efficiency is low

Method used

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  • Preparation method of vitamin K2
  • Preparation method of vitamin K2

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A preparation method of vitamin K2, comprising the steps of:

[0028] (1) Insert the culture medium of Bacillus natto as follows in the fermenter: glucose 2g / L, peptone 20g / L, ammonium sulfate 0.5%, K 2 HPO 4 2g / L, KH 2 PO 4 1g / , CaCl 2 0.1g / L, MgSO 4 1g / L. Then, the Bacillus natto was inserted into the culture medium, and the fermentation conditions were as follows: the fermentation temperature was 37° C., the initial pH value was 8.0, the inoculum size was 6%, and the fermentation time was five days.

[0029] (2) According to the ratio that the concentration of the composite flocculant in the fermentation broth is 50mg / L, add the composite flocculant (by polyaluminum chloride and 2Na 2 CO 3 ·3H 2 o 2 Mix according to the ratio of mass ratio of 5:1), stir and let it stand, after the Bacillus natto cells settle, use a tube with filtering function to extract the supernatant liquid from the upper layer, which is the liquid to be extracted, and set aside.

[...

Embodiment 2

[0034] A preparation method of vitamin K2, comprising the steps of:

[0035] (1) Insert the culture medium of Bacillus natto as follows in the fermenter: glucose 2g / L, peptone 20g / L, ammonium sulfate 0.5%, K 2 HPO 4 2g / L, KH 2 PO 4 1g / , CaCl 2 0.1g / L, MgSO 41g / L. Then, the Bacillus natto was inserted into the culture medium, and the fermentation conditions were as follows: the fermentation temperature was 37° C., the initial pH value was 7.5, the inoculum size was 7.5%, and the fermentation time was five days.

[0036] (2) According to the concentration of the composite flocculant in the fermented liquid is the ratio of 45mg / L, add the composite flocculant (by polyaluminum chloride and 2Na 2 CO 3 ·3H 2 o 2 Mix according to the ratio of mass ratio of 8:1), stir and let it stand, after the Bacillus natto cells settle, use a tube with filtering function to extract the supernatant liquid from the upper layer, which is the liquid to be extracted, and set aside.

[0037...

Embodiment 3

[0041] A preparation method of vitamin K2, comprising the steps of:

[0042] (1) Insert the culture medium of Bacillus natto as follows in the fermenter: glucose 2g / L, peptone 20g / L, ammonium sulfate 0.5%, K 2 HPO 4 2g / L, KH 2 PO 4 1g / , CaCl 2 0.1g / L, MgSO 4 1g / L. Then, the Bacillus natto was inserted into the culture medium, and the fermentation conditions were as follows: the fermentation temperature was 37° C., the initial pH value was 7.5, the inoculum size was 5%, and the fermentation time was five days.

[0043] (2) According to the ratio that the concentration of the composite flocculant in the fermentation broth is 60mg / L, add the composite flocculant (by polyaluminum chloride and 2Na 2 CO 3 ·3H 2 o 2 Mix according to the ratio of 2.5:1 in mass ratio), stir and let it stand, after the Bacillus natto cells settle, use a tube with filtering function to extract the upper clear liquid, which is the liquid to be extracted, and set aside.

[0044] (3) Concentra...

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Abstract

The invention relates to the technical field of vitamin K2, and particularly relates to a preparation method of vitamin K2. The preparation method comprises the following steps of: (1) adding a composite flocculant into a fermentation liquid of bacillus natto, then performing solid-liquid separation, and collecting a liquid as a to-be-extracted liquid for later use; (2) concentrating the to-be-extracted liquid into a concentrated liquid for later use; (3) freeze-drying the concentrated liquid to obtain a freeze-dried powder containing a vitamin K2 crude product for later use; (4) dissolving the freeze-dried powder in acetone, adding water as a precipitant after the freeze-dried powder is completely dissolved, precipitating the vitamin K2, separating out the precipitate, removing the acetone in the remaining liquid, and recovering the remaining liquid as a mother liquid for later use; and (5) combining the mother liquid with the concentrated liquid prepared in the next step (3) to realize the recycling of the mother liquid. The method provided by the invention not only can effectively improve the purification purity of the vitamin K2, but also can realize the effective utilization of the mother liquid after the vitamin K2 is extracted.

Description

technical field [0001] The invention relates to the technical field of vitamin K2, in particular to a vitamin K 2 method of preparation. Background technique [0002] Vitamin K2 (VitaminK2, referred to as VK2) is a general term for a series of terpene side chain compounds containing a 2-methyl-1,4-naphthoquinone core and a C3 position with varying numbers of isoprene structural units. Vitamin K2 is mostly found in fermented foods, such as natto, which is a soybean product fermented from soybeans, rich in various nutrients, especially high content of vitamin K2. Vitamin K2 can help carboxylate the primary osteocalcin secreted by osteoblasts and turn it into active osteocalcin, thereby promoting the deposition of calcium ions in the blood into the bone. Vitamin K2 produces bone protein, and then together with calcium to form bone, can increase bone density and prevent fractures. In addition, vitamin K2 also has the effect of preventing liver cirrhosis from progressing to li...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/66C07C46/10C12R1/07
CPCC12P7/66C07C46/10C07C50/14
Inventor 卢伟刘长峰韩宁王庆师迎祥赵鹏
Owner SHANDONG RUNDE BIOTECH CO LTD
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