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Carbon-based electrochemical sensor for detecting heavy metal lead ions and application thereof

A carbon-based electrochemical and lead ion technology, which is applied in the field of environmental pollutant analysis and heavy metal ion detection, can solve the problems of increasing the detection signal, achieve the effects of reducing background current, improving selectivity and sensitivity, and stabilizing load performance

Pending Publication Date: 2021-09-17
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, how to increase the detection signal and sensitivity has become a bottleneck in the detection of lead ions by electrochemical methods.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0027] The invention provides a method for preparing a carbon-based electrochemical sensor for detecting heavy metal lead ions, the method comprising the following steps:

[0028] 1) Pretreat the hydrophobic carbon paper, and then use cyclic voltammetry to electrodeposit and reduce graphene oxide to obtain a CP / rGO electrode;

[0029] 2) The dsDNA solution was drip-coated on the surface of the CP / rGO electrode, and then dried to obtain the CP / rGO / DNA carbon-based electrochemical sensor.

[0030] In step 1), the pretreatment process of the hydrophobic carbon paper is: ultrasonically cleaning the hydrophobic carbon paper with ethanol and water in sequence, and sealing the non-deposition area of ​​the hydrophobic carbon paper with insulating glue after drying. The process of using cyclic voltammetry to electrodeposit and reduce graphene oxide is as follows: immerse the pretreated hydrophobic carbon paper in the graphene oxide suspension, use cyclic voltammetry to electrodeposit a...

Embodiment 1

[0035] The preparation method of the carbon-based electrochemical sensor for detecting heavy metal lead ions is as follows:

[0036](1) Pretreatment of carbon paper. The 0.9 cm × 1.7 cm hydrophobic carbon paper was ultrasonically cleaned in absolute ethanol and deionized water for 5 min, and dried to obtain a clean carbon paper surface. Seal the other parts of the carbon paper with insulating glue, leaving a conductive area of ​​2 mm × 2 mm to obtain a CP electrode.

[0037] (2) Preparation of CP / rGO electrode. Graphene oxide (GO) was ultrasonically dispersed in 0.1 mol / L PBS buffer solution with pH=9.18 for 1.5 h to form a homogeneous suspension with a concentration of 0.5 mg / mL. The obtained CP electrode was immersed in the above GO suspension, and rGO was electrodeposited by cyclic voltammetry (CV) with a scanning range of -0.96-0.78V and a scanning speed of 0.1V / s for 5 cycles. After scanning, the electrode was immersed in deionized water to wash off the unstable GO on ...

Embodiment 2

[0041] The CP / rGO / DNA electrochemical sensor prepared in Example 1 was used for electrochemical detection of lead ions.

[0042] Differential pulsed anodic stripping voltammetry (DPASV) was used to detect lead ions. A three-electrode system was used. The CP / rGO / DNA electrochemical sensor was used as the working electrode, the saturated calomel electrode was used as the reference electrode, and the platinum sheet was used as the counter electrode. First, a series of lead ion standard buffer solutions with different concentrations were deposited at a constant potential under stirring conditions, with a deposition potential of -0.1V and a deposition time of 360s. Then, it is dissolved in a blank buffer solution without lead ions, and the stripping potential scanning range is -0.8-0V, and the peak current of anodic stripping is obtained. When the concentration of lead ions increases, the peak current value increases accordingly, because the amount of lead ions deposited on the ele...

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Abstract

The invention relates to a carbon-based electrochemical sensor for detecting heavy metal lead ions and application thereof. The preparation method of the carbon-based electrochemical sensor comprises the following steps: 1, perprocessing hydrophobic carbon paper, enabling graphene oxide to be subjected to electrodeposition reduction through cyclic voltammetry, and obtaining a CP / rGO electrode; and 2) dispensing a dsDNA solution on the surface of the CP / rGO electrode, and then performing drying to obtain the CP / rGO / DNA carbon-based electrochemical sensor which is used for detecting the concentration of heavy metal lead ions in a water environment. Compared with the prior art, the sensitivity and selectivity of the sensor are improved by utilizing the characteristic that DNA and lead ions can be specifically combined, and the loading capacity of the DNA is increased by utilizing pi-pi accumulation and hydrophobic interaction between rGO and DNA; the anodic stripping peak current of the differential pulse anodic stripping voltammetry is adopted as a quantitative detection basis, the detection sensitivity can be effectively enhanced, the detection limit is as low as 1 pM, and the detection method is rapid, simple and convenient and can be used for detection and analysis of trace heavy metal lead ions.

Description

technical field [0001] The invention belongs to the technical field of environmental pollutant analysis and heavy metal ion detection, and relates to a carbon-based electrochemical sensor for detecting heavy metal lead ions and an application thereof. Background technique [0002] Heavy metal ion pollution has attracted widespread attention in today's world, especially lead ions. Lead-containing materials are widely used in the manufacture of various daily life and industrial production supplies, such as pigments, lead batteries, coal combustion, etc. my country's "Drinking Water Hygienic Standards" stipulates that the lead content in drinking water should not exceed 0.01mg / L, which is consistent with the regulations of the World Health Organization. Lead will not only cause serious pollution to the environment, but also damage human health, especially children. According to a report released by UNICEF and Pure Earth in July 2020, about one-third of children (up to 800 mill...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/48G01N27/30G01N27/327
CPCG01N27/48G01N27/308G01N27/3277
Inventor 刘梅川张紫薇袁禁郑慧玲孙欢欢赵国华
Owner TONGJI UNIV
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