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Method for evaluating toxicity of tropane drugs based on zebra fish model

A zebrafish and toxicity technology, which is applied in the fields of pharmaceutical formulation, compound screening/testing, preparations for in vivo experiments, etc., can solve immature poisoning, hallucinations, convulsions, and the negative effects of tropane alkaloids are not very clear, etc. problem, to achieve the effect of easy operation and simple treatment method

Pending Publication Date: 2021-09-21
ZHEJIANG POLICE COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At the same time as drugs, some tropane hydrocarbon alkaloids are highly toxic. Excessive use will lead to coma and even death. They are even used in crimes. Symptoms of poisoning generally include hallucinations and convulsions. However, the current identification and evaluation technology of poisoning It is still immature, and the negative effects of tropane alkaloids on organisms are also not very clear, so it is very important to establish a reasonable drug toxicity evaluation system

Method used

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  • Method for evaluating toxicity of tropane drugs based on zebra fish model
  • Method for evaluating toxicity of tropane drugs based on zebra fish model
  • Method for evaluating toxicity of tropane drugs based on zebra fish model

Examples

Experimental program
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Effect test

Embodiment 1

[0066] (1) Zebrafish embryo acute toxicity experiment

[0067] Randomly select 150 wild-type AB strain zebrafish 2 days after fertilization (2dpf) in a six-well plate, 30 fish per well (experimental group), and give (-) scopolamine 35, 104, 313 and 350 μg / mL concentrations in water respectively At the same time, a normal control group (E3 buffer solution treatment) was set, and the capacity of each well (experimental group) was 3 mL. The zebrafish were continuously incubated in a 28°C incubator for 3 days, and the number of dead zebrafish in each experimental group was observed and counted every day and removed in time. After the experiment, observe and record the zebrafish heart, brain, ear, mandible, eye, liver, intestine, trunk / tail / notochord, muscle / body segment, fin, body length, body coloring, and circulation of each experimental group under a microscope System, body edema and hemorrhage and other toxic reactions, the incidence of toxicity in each experimental group was...

Embodiment 2～8

[0074] The operation method of the present embodiment is basically the same as that of Example 1, using respectively atropine, scopolamine, (-) scopolamine hydrobromide solution, racemic anisodamine solution, anisodine hydrobromide solution, α-tropine alcohol solution and Scopoletin solution was used as the test drug, the results are shown in Figure 4-24 and Table 1, where, Figure 4 (-) "concentration-mortality" effect curve of atropine on behavioral toxicity in zebrafish, Figure 5 For the effect of atropine on the motor function of zebrafish, Figure 6 is the effect of atropine on the movement speed per minute of zebrafish, Figure 7 is the "concentration-mortality" effect curve of scopolamine on zebrafish acute toxicity, Figure 8 The effect of scopolamine on the motor function of zebrafish, Figure 9 is the effect of scopolamine on the movement speed per minute of zebrafish, Figure 10 For the effect of (-) scopolamine hydrobromide on the motor function of zebrafish...

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Abstract

The invention discloses a method for evaluating the toxicity of tropane drugs based on a zebra fish model. The method comprises the following steps: (1) preparing a tropane drug solution; (2) treating zebrafish embryos, juvenile zebrafish and adult zebrafish by adopting the tropane drug solution to obtain a toxic reaction result; wherein the toxic reaction result comprises a zebra fish juvenile fish embryonic development toxicity result, a zebra fish juvenile fish behavioral toxicity result and a zebra fish adult fish behavioral toxicity result; and (3) obtaining toxicity data of the tropane drugs according to the toxic reaction result.

Description

technical field [0001] The invention belongs to the field of toxicant analysis, in particular to a method for evaluating the toxicity of tropane hydrocarbon drugs based on a zebrafish model. Background technique [0002] Tropane alkaloids are a class of natural products with strong physiological activity, and currently occupy an important position in medicine. my country has abundant animal and plant resources of tropane alkaloids, and a large number of alkaloids have been put into use, including naturally occurring alkaloids and artificially synthesized derivatives. It is widely used in medical treatment, such as atropine, scopolamine, anisodamine and anisodamine and other tropane alkaloid drugs, which are one of the most commonly used drugs in medical institutions. Active microcirculation and detoxification of phosphorus and other aspects of the role. At the same time as drugs, some tropane hydrocarbon alkaloids are highly toxic. Excessive use will lead to coma and even ...

Claims

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Application Information

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IPC IPC(8): A61K49/00
CPCA61K49/0004
Inventor 王斌杰陈天逸汪安利吴元钊姚伟宣王继业许中石
Owner ZHEJIANG POLICE COLLEGE
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