Composite cross-linked bioprosthetic valve and preparation method thereof
A bioprosthetic valve and composite technology, applied in tissue regeneration, medical science, prosthesis, etc., can solve the problems of inability to endothelialize, toxicity, etc., achieve good anti-collagen enzymolysis and elastolysis ability, thin thickness, and wide application range effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0043] Example 1 Decellularization of fresh bovine pericardium
[0044]Soak fresh bovine pericardium with 0.1% bromogeramine, disinfect for 30 minutes, and stir once every 10 minutes;
[0045] Place the sterilized fresh bovine pericardium in a solution with a mass percent concentration of 0.25% Trixon-100, shake it at 37°C for 48 hours, and change the solution every 12 hours;
[0046] Use 3U / mL of DNase, 0.03mg / mL of RNase, 2.5mmol / L of MgCl 2 , 0.1mmol / L of CaCl 2 Prepare a mixed solution, place the bovine pericardium treated with Trixon-100 in the mixed solution, shake at 37°C for 24 hours, and change the solution every 12 hours;
[0047] Then use distilled water to shake and rinse for 48 hours, and change the solution every 8 hours;
[0048] Then use PBS buffer to rinse for 24 hours, change the medium, and then use new PBS buffer to preserve the decellularized bovine pericardium.
[0049] Among them, the PBS buffer formula is: per 10XPBS 1L: NACL 80g, KCL 2g, KH 2 PO ...
Embodiment 2
[0050] Example 2 Cross-linking decellularized bovine pericardium
[0051] 1) The decellularized bovine pericardium obtained in Example 1 was placed in a 1% tannic acid solution (using 70% ethanol-water solution as a solvent, using hydrochloric acid to adjust pH=5), at room temperature (25 ±5°C) shaking treatment for 48h;
[0052] 2) Put tannic acid-decellularized bovine pericardium in 1mmol / L neomycin solution (use 0.05mml / L morpholineethanesulfonic acid solution as solvent, use hydrochloric acid to adjust pH=6), normal temperature (25± 5°C) for 1 h with shaking.
[0053] 3) Put tannic acid-neomycin-decellularized bovine pericardium in 10 mmol / L riboflavin 5-monophosphate solution (using PBS buffer as a solvent), and irradiate for 12 hours under ultraviolet light with a wavelength of 360 nm;
[0054] 4) Rinse with PBS buffer, and then store with new PBS buffer.
[0055] Among them, the PBS buffer formula is: per 10XPBS 1L: NACL 80g, KCL 2g, KH 2 PO 4 2g, NA 2 HPO 4 *12...
PUM
Property | Measurement | Unit |
---|---|---|
wavelength | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com