Ultralow-temperature cryopreservation method for fenneropenaeus chinensis sperms

A technology of ultra-low temperature freezing and preservation method, which is applied in the field of ultra-low temperature cryopreservation of Chinese prawn sperm, which can solve the problems of poor repeatability and general effect, and achieve the effects of increasing viscosity, reducing the formation of ice crystals, and protecting ice crystals and osmotic pressure damage
CN113455497AActive Publication Date: 2021-10-01山东省海洋科学研究院
1 Cites 2 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
山东省海洋科学研究院
Publication Date
2021-10-01

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention relates to an ultralow-temperature cryopreservation method for fenneropenaeus chinensis sperms, and belongs to the technical field of sperm preservation. The method specifically comprises the following steps: diluting collected fenneropenaeus chinensis semen with an ultralow-temperature freezing protection solution, performing programmed cooling to-180 DEG C, and transferring into liquid nitrogen for preservation. According to the method, the fenneropenaeus chinensis semen diluted by the ultralow-temperature freezing protection liquid is subjected to programmed cooling to-180 DEG C and then is transferred into liquid nitrogen for preservation, the sperm stops metabolic activity in an ultralow-temperature environment so as to prolong the service life. The ultralow-temperature freezing protection liquid is combined with water molecules in the solution to initiate the hydration action. The crystallization process of water is weakened to increase the viscosity of the solution so as to reduce the formation of ice crystals. Meanwhile, a certain molar concentration can be maintained inside and outside cells to reduce the concentration of electrolyte in an unfrozen solution inside and outside the cells, so that the cells are prevented from being damaged by solutes, and the cells are protected from being damaged by the solution and the ice crystals.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to the technical field of sperm preservation, in particular to a method for ultra-low temperature freezing and preservation of Chinese penaeus prawn sperm. Background technique

[0002] Chinese prawn (Fenneropenaeus chinensis), also known as Chinese prawn, formerly known as Chinese prawn, belongs to Arthropoda, Crustacea, Decapoda, Penaeidae, Penaeus, It is one of the main cultured shrimp species in my country. The individual is larger, the carapace is thin, smooth and transparent, the shrimp meat is crispy and soft, and it has extremely high economic value. However, in recent years, due to the increasing demand year by year, the wild population of Penaeus prawns in China has begun to decrease sharply year by year, relying heavily on artificial breeding and release. Therefore, it is very necessary to carry out germplasm preservation of Penaeus prawn, and then carry out germplasm optimization and establish a high-quality species b...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More