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A strain of Bacillus siamese that degrades oil and its application in oily wastewater

A bacillus and oil technology, applied in the field of environmental microorganisms, can solve problems such as oil degradation of Bacillus siamese that have not yet been reported

Active Publication Date: 2021-12-07
FOSHAN YUHUANG ECOLOGICAL ENVIRONMENT TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many types of microorganisms that have the function of degrading oil, such as Pseudomonas, Klebsiella oxytoca, Staphylococcus saprophyticus, Serratia marcescens, yeast, mold, etc. Bacillus for Degradation of Oil in Wastewater

Method used

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  • A strain of Bacillus siamese that degrades oil and its application in oily wastewater
  • A strain of Bacillus siamese that degrades oil and its application in oily wastewater
  • A strain of Bacillus siamese that degrades oil and its application in oily wastewater

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Effect test

Embodiment 1

[0058] Embodiment 1: Screening, performance verification and identification of Bacillus siamese BS1

[0059] For the Bacillus siamese BS1 that degrades grease according to the present invention, the screening samples are collected from kitchen waste and grease-containing wastewater.

[0060] Enrichment culture:

[0061] Take 5 mL of the collected sample, add it to a Erlenmeyer flask filled with 100 mL of enrichment medium, and place it on a constant temperature culture shaker for cultivation.

[0062] Enrichment medium composition: soybean oil 10 g / L (soybean oil brand: Arowana, the soybean oil used below is this brand), trypsin 5 g / L, yeast powder 10 g / L, sodium chloride 10 g / L L; adjust the pH value to 6.8.

[0063] The culture conditions were 30 °C, 200 rpm, and the culture was finished after 36 h. After the end, 5 mL of the culture was taken out and inoculated into fresh enriched medium for subculture. The subculture conditions were consistent with the above-mentioned c...

Embodiment 2

[0086] Embodiment 2: the performance of bacillus siamese BS1 at different temperatures

[0087] Bacillus siamese BS1 was inoculated in the oil medium containing 2 g / L soybean oil, the culture temperature was controlled at 30 °C, the constant temperature culture shaker speed was 200 rpm, and the culture was completed for 12 h. The resulting cultures were used as seeds for subsequent validation.

[0088] The cultivated seeds were respectively inoculated in the oil medium containing 100 mL of soybean oil with a concentration of 10 g / L according to the inoculum amount of 5%. After inoculation, 10 mL of the medium, that is, the 0 h sample, was used to detect the initial oil concentration . Then the Erlenmeyer flasks were cultured in constant temperature culture shakers at temperatures of 15°C, 25°C, 30°C, 37°C, 40°C, and 45°C, and the speed of the constant temperature culture shaker was controlled at 200 rpm. During the cultivation process, 1 mL samples were taken every 6 h until...

Embodiment 3

[0090] Embodiment 3: the performance of bacillus siamese BS1 under different dissolved oxygen

[0091] Bacillus siamese BS1 was inoculated in the oil medium containing 2 g / L soybean oil, the culture temperature was controlled at 37 °C, the constant temperature culture shaker speed was 200 rpm, and the culture was completed for 12 h. The resulting cultures were used as seeds for subsequent validation.

[0092] The cultivated seeds were respectively inoculated in the oil medium containing 100 mL of soybean oil with a concentration of 10 g / L according to the inoculum amount of 5%. After inoculation, 10 mL of the medium, that is, the 0 h sample, was used to detect the initial oil concentration . Then the Erlenmeyer flasks were cultured in constant temperature culture shakers with rotation speeds of 100 rpm, 150 rpm, 200 rpm, and 250 rpm respectively, at 37 °C, and the speed of the constant temperature culture shaker was controlled at 200 rpm. During the cultivation process, 1 mL...

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Abstract

The invention discloses a siamese bacillus that degrades oil and its application in oily wastewater. The Bacillus siamese BS1 disclosed in the present invention has a preservation number in the China Center for Type Culture Collection as CCTCC NO: M 2021840. The Bacillus siamensis BS1 of the present invention can be used to degrade oil and fat. In the oil medium containing soybean oil and oil with an initial concentration of 9.5-10.5g / L, the culture temperature is controlled at 37°C, the constant temperature culture shaker speed is 200 rpm, and the culture time is 48 After h, the degradation rate of oil reached 82.34%. The invention also discloses the application of Bacillus siamensis BS1 in oil-containing wastewater, which has better oil degradation ability.

Description

technical field [0001] The invention belongs to the technical field of environmental microbes, and in particular relates to a bacillus siamese strain degrading oil and its application in oil-containing wastewater. Background technique [0002] With the improvement of people's living standards and the rapid development of the catering industry, the content of oil in catering is increasing, and the discharge of catering wastewater is also increasing. The oil contained in the wastewater is discharged into the environment, which will pollute the environment, form an oil film on the water surface, affect the dissolved oxygen in the water body, and endanger the aquatic organisms; at the same time, oil is a kind of organic matter, and the discharge into the environment will lead to eutrophication of the water body; in addition , Grease is highly viscous, and it is easy to adhere to the inner wall of the wastewater transportation pipeline, and accumulate over time, thereby blocking ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/07C02F101/34C02F103/32
CPCC12N1/20C02F3/34C02F2101/34C02F2103/322
Inventor 邱建贺翁雯梁锡伟周国彪王春莲陈健辉
Owner FOSHAN YUHUANG ECOLOGICAL ENVIRONMENT TECH
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