Application of isosteviol in preparation of cosmetics for whitening skin and anti-melanin

A technology of melanin inhibitor and isosteviol, which is applied in the field of cosmetics in the field of daily chemicals, and can solve problems such as fever, skin sensitivity, and contact dermatitis

Pending Publication Date: 2021-10-08
ZHUHAI YUANZHI HEALTH TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, after long-term use, many people began to experience si

Method used

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  • Application of isosteviol in preparation of cosmetics for whitening skin and anti-melanin
  • Application of isosteviol in preparation of cosmetics for whitening skin and anti-melanin
  • Application of isosteviol in preparation of cosmetics for whitening skin and anti-melanin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: Zebrafish Embryo Melanin Content and Tyrosinase Experiment

[0023] Zebrafish embryos born within 2 h were collected. Observe and count, and remove dead and unsuccessfully fertilized zebrafish embryos. The washed healthy embryos (n=100-150) were transferred to a 100 mm sterile Petri dish containing 12 mL of 0.0002% (w / v) methylene blue embryo culture solution. The zebrafish embryos were developed at 28±1°C for 23 hours, and the dead, asynchronous and morphologically defective embryos were removed under an optical microscope to obtain the complete zebrafish embryos fertilized for 24 hours for the experiment. For dechorionized embryos for experiments, digest the chorion of zebrafish embryos with 1 mg / mL pronase. After 2-3 minutes, the chorion begins to fall off, and the embryo is washed with Holt buffer to remove all the chorion, and a healthy 24-hour fertilized zebrafish chorion-free embryo is obtained.

[0024] Relative content of melanin = (OD 450 drug g...

Embodiment 2

[0030] Example 2: qRT-PCR determination of gene expression levels related to melanin production

[0031] Obtain 24hpf chorion-free embryos, group embryos and administer drugs, collect embryos treated with 2 mL of liquid medicine for 24 hours, wash with sterile PBS repeatedly for 3 times, then extract total RNA, perform reverse transcription cDNA and qRT-PCR experiments, and determine Transcript product levels of genes related to melanin production.

[0032] Isosteviol can down-regulate the expression of mitfb, tyr, tyrp1b and trp2 genes, thereby reducing the production content of melanin. The positive control group, PTU, is a direct inhibitor of tyrosinase activity and has no effect on gene expression levels.

Embodiment 3

[0033] Embodiment 3: mouse skin melanoma B16-F10 cell experiment

[0034] The B16-F10 cells of passage 9-11 were used for the experiment and divided into 4 groups: normal control group, isosteviol administration group, and positive control group Arbutin and PTU. To detect the effect of isosteviol on melanin toxicity, production and tyrosinase activity in B16F10 cells.

[0035] Table 3 Effect of isosteviol on cell proliferation rate / toxicity of B16-F10 cells (X±SEM). N=3, compared with normal group, *p<0.05, **p<0.01, ***p<0.001.

[0036]

[0037] Table 3 Effect of isosteviol on melanin production content in B16-F10 cells (X±SEM). N=3, compared with normal group, *p<0.05, **p<0.01, ***p<0.001.

[0038]

[0039]

[0040] Routinely cultured for 48 hours, discarded the supernatant medium, washed once with PBS, and added 100 μL of enzymatic reaction substrate solution to each well. After incubation in the incubator for 4 hours, the OD value was measured at a wavelength...

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Abstract

The invention relates to a novel application of isosteviol in preparation of cosmetics for whitening skin and inhibiting melanin, and the cosmetics have a more remarkable effect and lower toxicity.

Description

technical field [0001] The invention relates to the application of isosteviol in the preparation of cosmetic preparations for skin whitening and anti-melanin production, in particular to the application of cosmetics in the field of daily chemicals. Background technique [0002] Melanin is a kind of irregular polyphenolic polymer, which is obtained from tyrosine or levodopa through a series of enzymatic reactions. The color of hair, skin, eyes, and even the shell of animals depends to a large extent on the quantity and distribution concentration ratio of melanin. Melanin has multiple biological functions, it can regulate temperature and protect the skin from ultraviolet rays, free radicals and reactive oxygen species. Hyperpigmentation in human skin can cause skin diseases such as freckles, dark spots, sun spots, melasma, and age spots. These can affect the individual's appearance and, in severe cases, can lead to negative emotions and psychological problems, thereby reduci...

Claims

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Application Information

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IPC IPC(8): A61K8/35A61Q19/02A61K31/19A61P17/00
CPCA61K8/35A61Q19/02A61K31/19A61K9/0053A61K9/0014A61P17/00
Inventor 陈凯欣邹小敏王东方谭文
Owner ZHUHAI YUANZHI HEALTH TECH CO LTD
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