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A Bioelectrochemical Method for Simultaneous Detection of Nitrite and Biochemical Oxygen Demand

A technology of bioelectrochemistry and nitrite, which is applied in the field of bioelectrochemistry to simultaneously detect nitrite and biochemical oxygen demand, can solve the problem of high detection cost, achieve the effect of improving accuracy and reducing device cost

Active Publication Date: 2022-05-27
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, when using bioelectrochemical sensor technology to detect different redox substances, it is often necessary to construct different bioelectrochemical sensor devices, which makes the detection cost high when using bioelectrochemical sensor technology to detect sewage containing various pollutants.

Method used

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  • A Bioelectrochemical Method for Simultaneous Detection of Nitrite and Biochemical Oxygen Demand
  • A Bioelectrochemical Method for Simultaneous Detection of Nitrite and Biochemical Oxygen Demand
  • A Bioelectrochemical Method for Simultaneous Detection of Nitrite and Biochemical Oxygen Demand

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] Using the sludge water of the anoxic section of the sewage plant as the inoculum, the bioelectrode for the bidirectional detector was cultivated in the MFC mode (ie, the microbial fuel cell mode):

[0071] The resistor 18 is connected externally to the bioelectrochemical reactor, and the loop is connected with a copper wire. The bioelectrochemical reactor was inoculated with sludge water in the anoxic section. The inoculation process lasted for five days, and then it was transferred to the sequencing batch mode for cultivation, and the matrix solution was changed every day. The voltage of the MFC was recorded by a digital data collection instrument (KEYSIGHT model 34970A).

[0072] Specifically: the bioelectrode 12 in the bioelectrochemical reactor is used as the anode, the non-biological electrode 13 is used as the cathode, the external resistor 18 and the copper wire form a loop, such as Pic 4-1 As shown, the bioelectrochemical reactor is operated in closed circuit ...

Embodiment 2

[0077] The bioelectrode is tested in different solutions, and the most suitable BOD and nitrite detection potentials are selected according to the test results during the potential step chronoamperometry test:

[0078] The bioelectrochemical reactor is connected with a potentiostat 19 in a three-electrode system to construct a bioelectrochemical sensor, wherein a graphite electrode (bioelectrode), a foamed nickel air electrode and a silver / silver chloride electrode are used as working electrodes, respectively, Counter and reference electrodes. The bioelectrodes were placed in 50mM PBS, and 100mg BOD L was added. -1 sodium acetate in 50 mM PBS, and add 20 mg NO 2 - -NL -1 CV tests were performed in 50 mM PBS with nitrite. The scanning range of CV is -0.6V~-0.1V(vs.Ag / AgCl), and the scanning speed is 0.5mV s -1 . Among them, in the subsequent test process of the constructed bioelectrochemical sensor (including the test of the solution containing a single pollutant and the ...

Embodiment 3

[0081] Potential step chronoamperometry is performed on the bioelectrode in solutions containing different nitrite concentrations and solutions with different BOD concentrations, and the signal output time point is selected according to the test results:

[0082] The bioelectrochemical reactor was connected with a potentiostat in a three-electrode system, graphite electrode (bioelectrode), foamed nickel air electrode and silver / silver chloride electrode were used as working electrode, counter electrode and reference electrode, respectively. by adding 12.5mL -1 Trace Minerals and 5mL L -1 The standard nitrite-containing solution was prepared by adding nitrite to 50mM PBS of vitamins, and the concentration of the standard nitrite solution was set to 2mgNO respectively. 2 - -N L -1 ,4mgNO 2 - -N L -1 ,8mgNO 2 - -N L -1 , 12mgNO 2 - -N L -1 and 16mgNO 2 - -N L -1 . by adding 12.5mL L -1 Trace Minerals and 5mL L -1 The standard BOD-containing solution was prepared...

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Abstract

The invention discloses a bioelectrochemical method for synchronous detection of nitrite and biochemical oxygen demand, including 1): a bioelectrochemical reactor operated in a closed circuit in MFC mode, adding an inoculum solution to an anode chamber; cultivating a bioelectrode in a substrate solution until maturity ; 2): The bioelectrochemical sensor is connected with the potentiostat to construct the bioelectrochemical sensor, and the bioelectrode is tested by cyclic voltammetry in the solution containing nitrite and BOD organic matter respectively, and the BOD in the process of the potential step chronoamperometry is selected. Organic matter and nitrite detection potential; 3): The bioelectrode performs potential step chronoamperometry in solutions containing different concentrations of nitrite and BOD organic matter, selects the signal output time point, obtains electrical response and nitrite and BOD organic matter The concentration detection marking line; according to the electrical response of the bioelectrochemical sensor in the sewage containing nitrite or the sewage containing BOD organic matter, the concentrations of nitrite and BOD organic matter in the sewage are respectively obtained.

Description

technical field [0001] The invention relates to a pollutant detection technology based on bioelectrochemical reaction, belonging to the fields of biological sensors and water body pollutant detection. Oxygen demand (BOD), specifically relates to a bioelectrochemical method for simultaneous detection of nitrite and biochemical oxygen demand. Background technique [0002] With the development of urbanization, the impact of urban sewage discharge on the environment continues to intensify, and the treatment and detection of urban sewage has become a hot issue of concern. Two typical pollutants, nitrite and organic matter, are often contained in urban sewage. Among them, nitrite easily causes eutrophication of natural water bodies and is one of the important causes of diseases such as cancer and hypertension. Organic matter (commonly measured by BOD) will quickly consume the dissolved oxygen in the water body, breed anaerobic microorganisms, and cause the deterioration of water...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/48G01N27/327G01N27/30
CPCG01N27/48G01N27/327G01N27/308
Inventor 成少安林朱凡
Owner ZHEJIANG UNIV
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