Method for simultaneously determining four effective components in Gongying Qinglan granules

Abstract

The invention relates to a method for simultaneously determining four effective components in Gongying Qinglan granules. The method comprises the following steps: extracting a test sample by using 70% ethanol as an extraction solvent, and simultaneously determining the four main components at one time by using liquid chromatography. According to the invention, 278 nm, 324 nm and 346 nm are adopted as the wavelengths of the effective components, so baseline fluctuation is small, the response values of chlorogenic acid and caffeic acid are higher, and the response value of berberine hydrochloride is also taken into consideration; the method can be used for simultaneously determining the contents of chlorogenic acid, caffeic acid, baicalin and berberine hydrochloride in the Gongying Qinglan granules, and is rapid, sensitive, accurate and high in specificity; and the method can simultaneously determine the contents of the four components through one-time sample treatment, so not only is the reagent and time saved, but also working efficiency is improved.

Description

technical field [0001] The invention relates to a simultaneous determination method for four active components in Gongyingqinglan granules, belonging to the field of detection of active components in veterinary medicines. Background technique [0002] Gongying Qinglan Granules is a traditional Chinese veterinary medicine preparation, which is recorded in "Veterinary Drug Quality Standards" 2017 Edition (Chinese Medicine Volume) p97-98. The prescription is: Dandelion 200g, Folium Isatidis 200g, Radix Isatidis 200g, Honeysuckle 100g, Scutellaria baicalensis 100g, Phellodendron Phellodendri 100g, licorice 100g, Huoxiang 50g, gypsum 50g; the above 9 flavors, add water and decoct twice, combine the decoction, filter, and concentrate the filtrate to a clear paste with a relative density of 1.28-1.30. Take 1 part of the clear paste, add 1.6 parts of dextrin and 1 part of sucrose to make granules, dry, granulate, and make 900g. It is yellow-brown granules; bitter and slightly sweet....

AI Technical Summary

Problems solved by technology

[0013] Among the above standard methods, the three TLC identifications are caffeic acid, baicalin and berberine hydrochloride respectively. The pretreatment methods are different, and the solvents used are various and highly toxic: benzene-ethyl acetate-methanol-isopropanol- Concentrated ammonia test solution-butanone-n-butanol formic acid-glacial acetic acid, etc., are more harmful to experimenters

In addition, the pre-preparation of thin-layer chromatography in this scheme is complicated, and some require multiple steps such as pre-saturation, unfolding, drying, spraying, checking and checking the results, etc., which is time-consuming and labor-intensive, and is greatly affected by factors such as environmental temperature and humidity, and edge effects, etc. , thin-layer spots are sometimes difficult to distinguish, and the reproducibility is poor; therefore, thin-layer identification of spots is greatly affected by environmental temperature, humidity and human operation.

[0014] In the standard, only one component of chlorogenic acid is determined, and other active components cannot be detected at the same time

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