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Method for extracting phlorizin and phloretin from apple root bark and application of phlorizin and phloretin

A technology for phloretin and phloretin, which is applied in chemical instruments and methods, separation/purification of carbonyl compounds, sugar derivatives, etc., can solve problems such as waste, resource waste, loss, etc., and achieve the effect of improving the extraction rate

Active Publication Date: 2021-10-19
HUNAN NUSTREETCARAX
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In the prior art, there is only the method for preparing a single high-purity phloridin with apple or its extract, or the method for a single phloretin, and there is no method for obtaining two high-purity components at the same time. Like this, in the preparation of a component At the same time, it causes waste and loss of another component, which causes a lot of waste of resources during industrialized mass production.

Method used

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  • Method for extracting phlorizin and phloretin from apple root bark and application of phlorizin and phloretin
  • Method for extracting phlorizin and phloretin from apple root bark and application of phlorizin and phloretin
  • Method for extracting phlorizin and phloretin from apple root bark and application of phlorizin and phloretin

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Embodiment 1

[0052] The embodiment of the present invention provides a method for extracting phloridin and phloretin in apple root bark, specifically comprising the following steps:

[0053] (1) Dry extraction: 1 kg of apple root bark is vacuum freeze-dried, dried to a water content≤15%, freeze-dried, and desorbed by apple root bark with 30% ethanol at a solid-to-liquid ratio of 1:5 for 10 minutes. Then quickly add 1:15 boiling water for extraction, the extraction temperature is 82-100°C, and the extraction time is 1h;

[0054] (2) Purification: the temperature of the above-mentioned extract is lowered, adding 0.5% flocculant (polyacrylamide), and centrifuging to obtain a clarified centrifugate, which is filtered with a ceramic membrane (600 nm).

[0055] (3) Isolation of phloretin: drying the centrifugal precipitate, dissolving it with ethanol, concentrating and drying the purified resin, cooling and crystallizing with 20% ethanol, and recrystallizing to obtain phloretin.

[0056] (4) Ad...

Embodiment 2

[0062] The embodiment of the present invention provides a method for extracting phloridin and phloretin in apple root bark, specifically comprising the following steps:

[0063] (1) Dry extraction: 1 kg of apple root bark is vacuum freeze-dried, dried to a water content≤15%, freeze-dried, and desorbed by apple root bark with 30% ethanol at a solid-to-liquid ratio of 1:5 for 10 minutes. Then quickly add 1:15 boiling water for extraction, the extraction temperature is 82-100°C, and the extraction time is 1h.

[0064] (2) Purification: the temperature of the above-mentioned extract is lowered, adding 0.5% flocculant (polyacrylamide), and centrifuging to obtain a clarified centrifugate, which is filtered with a ceramic membrane (600 nm).

[0065] (3) Isolation of phloretin: drying the centrifugal precipitate, dissolving it with ethanol, concentrating and drying the purified resin, cooling and crystallizing with 20% ethanol, and recrystallizing to obtain phloretin.

[0066] (4) Ad...

Embodiment 3

[0072] The embodiment of the present invention provides a method for extracting phloridin and phloretin in apple root bark, specifically comprising the following steps:

[0073] (1) dry extraction: 2kg of apple root bark is subjected to vacuum freeze-drying treatment, dried to water content≤15%, freeze-dried, the apple root bark is desorbed with 30% ethanol, and the ratio of solid to liquid is 1:8, and the time is 20min. Then quickly add 1:20 boiling water for extraction, the extraction temperature is 82-100°C, and the extraction time is 1h.

[0074] (2) Purification: the temperature of the above-mentioned extract is lowered, adding 0.5% flocculant (polyacrylamide), and centrifuging to obtain a clarified centrifugate, which is filtered with a ceramic membrane (600 nm).

[0075] (3) Isolation of phloretin: drying the centrifugal precipitate, dissolving it with ethanol, concentrating and drying the purified resin, cooling and crystallizing with 20% ethanol, and recrystallizing t...

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Abstract

The invention discloses a method for extracting phlorizin and phloretin in apple root bark and application of the method. The method comprises the following steps: analyzing the apple root bark with ethanol, then adding boiling water for extraction, cooling the obtained extracting solution, then adding a flocculating agent, carrying out solid-liquid separation after reaction, and respectively extracting the phlorizin and the phloretin from filtrate and filter residues to obtain the phlorizin and the phloretin. Apple root bark is selected as a raw material, an internal boiling method is adopted, a small amount of desorption solvent permeates into the material, then a large amount of thermal solvent is added to boil and vaporize the desorption agent, internal convection is generated, and diffusion of effective components is enhanced; a spongy loose porous structure is formed by adopting a vacuum freeze-drying method, many heat-sensitive substances cannot be denatured or inactivated, the growth of microorganisms and the action of enzymes cannot be performed, and the original characters can be kept; vacuum freeze drying and an internal boiling method are combined, so effective components are fully diffused, and the extraction rate is remarkably improved.

Description

technical field [0001] The invention belongs to the technical field of extraction of plant active ingredients, and more specifically relates to a method for extracting phloridin and phloretin in apple root bark and an application thereof. Background technique [0002] Phlorizin belongs to polyphenols, which is dihydrochalcone glucoside in plant flavonoids in polyphenols; Biological activity, such as regulating blood pressure, blood sugar, protecting the heart, removing free radicals in the body, whitening, etc., and also has the characteristics of low toxicity. [0003] Phloretin is a dihydrochalcone compound, which is a natural active substance present in apples, pears and other fruits and various vegetables. Phloretin has been used as a natural skin whitening agent. It can inhibit tyrosinase activity, Melanin activity, skin whitening, lightening effect on various skin spots. According to the latest research, it also has anti-inflammatory, immunosuppressive, cardiovascula...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H1/08C07H15/203C07C45/78C07C49/83
CPCC07H1/08C07H15/203C07C45/78C07C49/83Y02P20/10
Inventor 张宝堂蒋乐文范斌
Owner HUNAN NUSTREETCARAX