Unlock instant, AI-driven research and patent intelligence for your innovation.

Preparation method of carbonized polymer dot material and application of carbonized polymer dot material in living cell lifetime imaging and super-resolution imaging

A technology of polymer dots, compounds for applications in biophotonics and nanomaterials science

Active Publication Date: 2021-10-22
SHENZHEN UNIV
View PDF2 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The embodiment of the present invention provides a preparation method of carbonized polymer dot material and its application in life-time imaging and super-resolution imaging of living cells, aiming to solve the problem that existing probes are not suitable for long-term super-resolution of living cells Imaging and Fluorescence Lifetime Imaging Issues

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of carbonized polymer dot material and application of carbonized polymer dot material in living cell lifetime imaging and super-resolution imaging
  • Preparation method of carbonized polymer dot material and application of carbonized polymer dot material in living cell lifetime imaging and super-resolution imaging
  • Preparation method of carbonized polymer dot material and application of carbonized polymer dot material in living cell lifetime imaging and super-resolution imaging

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Weigh 200 μmol (24.4 mg) of 2,4-diaminotoluene, 25 μmol (11 mg) of vitamin B9 (folic acid) and 20 μmol (2.3 mg) of betaine and disperse them in 25 mL of deionized water, and disperse evenly by ultrasonication. Add 25 μL of 10 mol / L hydrochloric acid to this solution, stir and mix well, then package in a 50 mL hydrothermal reactor with a pressure of 9 atmospheres, raise the temperature to 180°C and react for 20 hours to obtain a crude product solution. The crude product solution was collected by rotary evaporation to remove the solvent, then dialyzed for 24 hours (molecular weight cut-off 2000Da), and further purified by silica gel column chromatography (developing solvent: 1:9 methanol / dichloromethane mixed solvent). Collect the purified components, remove the solvent by rotary evaporation, disperse in deionized water, filter and lyophilize to obtain the solid powder of the target product. figure 2 It is the transmission electron microscope morphology and particle size...

Embodiment 2

[0048] Weigh 500 μmol (61 mg) of 2,6-diaminotoluene and disperse it in 5 mL of glycerin, and heat to 100 degrees Celsius under stirring until completely dissolved. After adding 50 μL of 1mol / L hydrochloric acid, further raise the temperature to 220°C, stir and react under normal pressure for 90 minutes, then cool to room temperature, add 20 mL of methanol for ultrasonic dispersion, and centrifuge to remove insoluble matter, take the supernatant, spin dry, and dialyze for 24 hours (molecular weight cut-off 2000Da) , and further purified by silica gel column chromatography (developing solvent: 1:9 methanol / dichloromethane mixed solvent). The purified fluorescent components were collected, and the target product was solid powder after the solvent was removed by rotary evaporation.

Embodiment 3

[0050] Weigh 500 μmol (61 mg) of 2,4-diaminotoluene and 50 μmol (22 mg) of vitamin B3 (nicotinic acid) and disperse them in 5 mL of glycerin, and heat to 100° C. under stirring until completely dissolved. After adding 50 μL of concentrated phosphoric acid, the temperature was further raised to 220°C, stirred and reacted under normal pressure for 30 minutes, then cooled to room temperature, 20 mL of methanol was added for ultrasonic dispersion, and the insoluble matter was removed by centrifugation. Purify by silica gel column chromatography (developing solvent: 1:9 mixed solvent of methanol / dichloromethane). The purified fluorescent components were collected, and the target product was solid powder after the solvent was removed by rotary evaporation.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
particle diameteraaaaaaaaaa
particle diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses a preparation method of a carbonized polymer dot material and application of the carbonized polymer dot material in living cell lifetime imaging and super-resolution imaging. The preparation method comprises the following steps: taking an aromatic amine compound as a main precursor raw material; and simultaneously dissolving or dispersing the main precursor raw material and an acid catalyst in a certain pure solvent or a mixed solvent to obtain a precursor solution, heating the precursor solution at normal pressure or high pressure, reacting for a period of time to obtain a crude product dispersion liquid, and further purifying and drying to obtain the carbonized polymer dot material. The carbonized polymer dot material prepared by the preparation method has the characteristics of good biocompatibility, low toxicity, low stimulated loss saturation intensity, high brightness, high optical stability, and high sensitivity and selective response to nucleic acid molecules, and is suitable for super-resolution imaging and lifetime imaging of nucleic acid structures in living cells based on STED and FLIM technologies.

Description

technical field [0001] The invention belongs to the fields of biophotonics and nanometer material science, and in particular relates to a preparation method of a carbonized polymer point material and its application in living cell lifetime imaging and super-resolution imaging. Background technique [0002] Nucleic acid macromolecules, including ribonucleic acid (RNA) and deoxyribonucleic acid (DNA), are the "blueprints" of genetic information for most organisms; observing changes in nucleic acid structure, shape, and spatial distribution through microscopic means is of great help in the study of growth, development, and apoptosis controlled by genes. vital processes such as death and necrosis. Fluorescence imaging microscopy represented by confocal imaging plays an important role in the application of nucleic acid imaging in live cells because the working conditions are compatible with the living conditions of living cells. In particular, the STED and FLIM microscopy techni...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C08G69/02C08G69/04C08G69/08C09K11/06G01N1/30G01N21/64
CPCC08G69/02C08G69/04C08G69/08C09K11/06G01N21/6486G01N1/30C09K2211/1466C09K2211/1425C09K2211/1483
Inventor 严伟刘彦峰彭晓屈军乐
Owner SHENZHEN UNIV
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com