Pantoea and bacterium manganese mixture and application of bacterium manganese mixture in degradation of malachite green

A malachite green and mixture technology, applied in the field of environmental microorganisms, can solve the problems of high treatment cost, secondary pollution, and low efficiency, and achieve the effects of broad application prospects, good ecological restoration, and low use cost

Active Publication Date: 2021-10-22
SHANDONG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of the high cost of malachite green sewage treatment in the prior art, low efficiency, and the technical problems of secondary pollution, one of the purposes of the present invention is to provide a strain of Pantoea eucrina SS01, which is composed of Pantoea eucrina SS01 and Pantoea eucrina SS01. The bacterial manganese mixture formed by mixing its manganese oxidation products, and the application of the bacterial manganese mixture in degrading malachite green

Method used

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  • Pantoea and bacterium manganese mixture and application of bacterium manganese mixture in degradation of malachite green
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  • Pantoea and bacterium manganese mixture and application of bacterium manganese mixture in degradation of malachite green

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Separation and Identification of Pan Pantoea Eucrina SS01

[0049] 1. Screening of PANTOEA Eucrina SS01 in Pantococcus strains

[0050] The flush PANTOEA Eucrina SS01 of the present invention is separated from salt ground leaves, and the separation method is:

[0051] 1) The collected salt alkali plant sample is selected from the upper part of the upper part of the top part, and then washed with water, dry and weigh the record.

[0052] 2) In the sterile operating table, it is placed in Tween 80 for 5 minutes, flushing 3 times with sterile water, and then soaking in Tween 80 for 3 minutes, flushing 3 times with sterile water, the above steps are repeated 3 to 5 times. Then 0.1% HGCl 2 Soak for 1 minute, 75% ethanol soaked for 3 minutes, and finally rinsed with sterile water. At the end, the water is coated with a plate and detects whether disinfection is thorough.

[0053] 3) Transfer the ulcerous experimental sample with aseptic twisted to the mortar of the sterilized bact...

Embodiment 2

[0063] Manganese mixture was prepared:

[0064] (1) Culture of strain SS01 scrubbing and seized the single colonies in the LB liquid medium, and the rotation of the rotation of 150 to 200 rpm was overnight in a shaker of 150 to 200 rpm to obtain a large amount of bacteria. Liquid; (2) Weigh MNCL 2 A total of 125.84 g is dissolved in 500 ml of deionized water, stirred at room temperature, and the final concentration of 2 m is obtained. 2 And filtering the sterilization; (3) Inoculation of 1% step (1) in the K culture medium, placed in 30 ° C, 120 rpm constant temperature clocked bed, per hour to measure OD 600 Value, wait for OD 600 When the value is 0.8, the 2m MnCl in step (2) is added to the shake flask. 2 The final concentration was 20 mM, and 3 days were continued, and the germ manganese mixture was further cultured; (4) centribus mixture obtained in step (3) was centrifuged at 4000 rpm for 15 min, discarded, washed twice with sterile deionized water. , Centrifugation was agai...

Embodiment 3

[0066] Example 1 The fung manganese mixture obtained by the fungus mixture was referred to as the balance, 10 mg per grant, 200 mg / L, 300 mg / L, 500 mg / L, 1000mg / L of the peacock green solution resuspended, placed at 30 ° C In the shaker of 120 rpm, it was shocked in 24 hours. After 24 hours, the peacock green residual content was measured, and three parallel experiments were set each experiment, each measurement three times, and the average value was taken. The whole process remains sterile. The degradation sample was centrifuged at 12000 rpm for 5 min, and the absorbed value was determined at 620 nm using an ultraviolet visible light spectrometer to calculate the degradation rate.

[0067] Calculation mode of degradation rate: The solution absorbance value is determined at 620 nm by the ultraviolet visible light spectrometer, calculating the degradation rate:

[0068] Degradation rate (%) = (a-b) / a × 100; the formula is the OD value before degradation, b is the OD value...

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Abstract

The invention relates to the technical field of environmental microorganisms, in particular to a pantoea, a bacterium manganese mixture and application of the bacterium manganese mixture in degradation of malachite green. The bacterial strain is preserved in the China Center for Type Culture Collection (called CCTCC for short, Luojia mountain on Bayi road, Wuchang District, Wuhan City, Hubei Province) on March 24, 2021, the preservation number is CCTCC M 2021491, the bacterial manganese mixture is formed by mixing Pantoea eucrina SS01 and a manganese oxidation product thereof, the Pantoea eucrina SS01 and a culture solution thereof are mixed with a manganese chloride solution for culture, and the cultured product is dried to obtain the bacterial manganese mixture. The bacteria-manganese mixture can rapidly degrade high-concentration malachite green and can continuously degrade the high-concentration malachite green, degradation products are nontoxic and harmless, the use cost is low, ecological restoration is good, the application prospect is extremely wide, and the practical problem that at present, triphenylmethane dye polluted water is difficult to treat can be solved.

Description

Technical field [0001] The present invention relates to the field of environmental microorganisms, and in particular, a germacry and germ marsher manganese mixture and the application in degradation of peacockstone green. Background technique [0002] The information disclosed in the background is disclosed only to increase the understanding of the overall background of the present invention, and is not necessarily considered to admit or imply that the information is intended to be a prior art known to those skilled in the art. [0003] Malchite Green, Mg is a basic trisenzyl dye, which is very susceptible to water, and its aqueous solution is blue and green. On the other hand, the peacock green as a dye is used in the staining of leather, paper and acrylic, and the other aspect is used as food additives and colorants in food, health care. In addition, Peacockstone has a broad spectrum bactericidal effect. It is widely used in the aquatic product breeding and other industries. It...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/30
CPCC12N1/20C02F3/34C02F2101/308
Inventor 赵国琰孙元凯王文静
Owner SHANDONG NORMAL UNIV
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