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Salmon skin polypeptide extraction method and application

An extraction method and fish skin technology are applied in the fields of economic specialty processing, salmon skin polypeptide extraction method and application research, which can solve the problems of restricting the application of terrestrial mammal collagen and its products, and achieve easy popularization and application and simple operation. , the effect of increasing solubility

Inactive Publication Date: 2021-10-22
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The meat of livestock, poultry and other organisms is one of the most important sources for human beings to obtain natural collagen and collagen peptides. Limiting people's application of terrestrial mammalian collagen and its products

Method used

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  • Salmon skin polypeptide extraction method and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1 A kind of extraction method of salmon skin polypeptide, its steps are as follows:

[0024] (1) Cleaning: process the dirt on the salmon skin surface with clear water;

[0025] (2) deodorization: step (1) is carried out deodorization process with activated carbon;

[0026] (3) degreasing: soak the fish skin and ether processed in step (2) with a certain solid-to-liquid ratio, and degrease with a rotary evaporator;

[0027] (4) Decolorization: Decolorization treatment with adsorption resin

[0028] (5) Extract fish skin protein, choose to choose a kind of in the most suitable protease from papain, pepsin, γ-trypsin; Experimental result sees figure 1 ,From figure 1 It can be seen that the effect of papain during pH5.5 is the best, therefore, the present embodiment selects papain.

[0029] (6) Enzymolyzing the extracted salmon skin protein to obtain a fish skin polypeptide hydrolyzate;

[0030] (7) Freeze-drying the polypeptide obtained in (6) for subseque...

Embodiment 2

[0033] Embodiment 2 A kind of salmon skin polypeptide application, its steps are as follows:

[0034] (1) Melt the polypeptide sample obtained in step (7) in PBS solution, and prepare 50, 100, 200ug / ml solutions for later use.

[0035] (2) Place the weighing bottle in an oven to constant weight, accurately weigh 100 mg of the sample into the weighing bottle, and then place it in a desiccator filled with saturated sodium carbonate solution. After 24, 48, 72, and 96 hours, Weigh its weight and measure its moisturizing effect.

[0036] (3) Cultivate B16F10 cells with DEME medium, wait for the cells to grow to 80-90% of the culture dish, digest with 0.125% trypsin, add the DEME of the polypeptide in step (7), continue to culture for 2-3 days, and collect the cells .

[0037] (4) Add PBS buffer containing 1% TritonX-100 to the cells, freeze and thaw repeatedly 3-4 times at -80°C and 37°C, centrifuge at 12000rpm, take the supernatant as the enzyme solution, and adjust the protein ...

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Abstract

The invention relates to a salmon skin polypeptide extraction method and application, and belongs to the technical field of economic specialty product processing, the method comprises the following steps: treating dirt on the surface of salmon skin with clear water; carrying out fishy smell removal treatment on the salmon skin by adopting active carbon; soaking the treated salmon skin and diethyl ether according to a certain solid-to-liquid ratio, and conducting degreasing by using a rotary evaporator; carrying out decolorization treatment by using adsorption resin; extracting salmon skin protein, and selecting one of pepsin, neutral protease, alkaline protease and trypsin; subjecting the extracted salmon skin protein to enzymolysis to obtain salmon skin polypeptide hydrolysate; and freeze-drying the obtained salmon skin polypeptide for later use. The fish skin polypeptide prepared by the method disclosed by the invention is high in nutritional value, relatively high in stability and safety, and capable of meeting the needs of consumers. Meanwhile, the technology is simple to operate and easy to popularize and apply.

Description

technical field [0001] The invention relates to a salmon skin polypeptide extraction method and the technical field of application research, and belongs to the technical field of special economic products processing. Background technique [0002] As a macromolecular polymer, collagen has been widely used in various fields, including pharmaceutical industry, health food industry, beauty products and so on. The meat of livestock, poultry and other organisms is one of the most important sources for human beings to obtain natural collagen and collagen peptides. It limits people's related application to terrestrial mammal collagen and its products. Therefore, it is becoming more and more important to research and develop new products to replace natural collagen. [0003] The amino acid composition and amino acid connection mode of aquatic organisms have their particularity, so the waste produced in the production process of aquatic products, such as collagen contained in skin a...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/14A61K8/64A61Q19/08
CPCC12P21/06C07K1/145A61K8/64A61Q19/08
Inventor 宋思祺陈伟
Owner SHANDONG AGRICULTURAL UNIVERSITY
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