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Retina protective agent, retina protection method and application of protective agent

A protective agent, retinal technology, applied in the field of retina, can solve problems such as treatment termination and recurrence of lesions, and achieve the effects of low price, inhibiting oxidative damage, and delaying tissue aging.

Pending Publication Date: 2021-11-02
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the problems existing in the prior art, the first purpose of the present invention is to provide a retinal protective agent, which contains ghrelin components inside, which can protect and improve the retinal damage that may be caused by diabetic patients. Compared with the traditional Anti-VEGF drugs and hormone drugs for the treatment of diabetic retinopathy are less prone to treatment termination and lesion recurrence, and the market price is also cheaper;

Method used

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  • Retina protective agent, retina protection method and application of protective agent
  • Retina protective agent, retina protection method and application of protective agent
  • Retina protective agent, retina protection method and application of protective agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 (study on the protective effect of exogenous Ghrelin on the retinal tissue of db / db mice):

[0033] Proceed as follows:

[0034] Step 1: Grouping and treatment of type 2 diabetic mice db / db: Take 10-week-old db / db mice, and use the tail artery to measure blood glucose (blood glucose ≥ 16.6 mmol / L) to identify whether the diabetes model is successful. Animal groups: control group, model group and Ghrelin group. Ghrelin group: In the last two weeks of the model group, a subcutaneous micropump was given to continuously pump Ghrelin (5 μg / kg / d) for two weeks. .

[0035] Step 2: HE staining to examine the retinal structure: Dewax the retinal tissue with xylene, prepare paraffin sections, dehydrate with gradient alcohol, rinse repeatedly with distilled water, soak in hematoxylin staining solution for 10 minutes, stain with ammonia water and acid water, and soak in gradient alcohol solution 10 min, HE staining for 30 s, and taking pictures after mounting.

[0036...

Embodiment 2

[0040] Example 2 (HE staining to observe pathological and morphological changes of retinal tissue):

[0041] Three rats were randomly selected in each group, and after the left eyeball was removed, part of the retinal tissue was fixed with 4% paraformaldehyde for 2 hours, and the 5 μm retinal slices were placed on a glass slide, washed with gradient alcohol, stained with H&E, and light microscope (200 ×) Take the image.

[0042] Histopathological changes of S retina:

[0043] Such as Figure 1-3 as shown, figure 1 In the control group, the retinal tissue was complete and the structure was clear; figure 2 The thickness of the retina in the model group was significantly reduced, and the inner and outer nuclear layer cells were loosely arranged; image 3 The thickness of the retina in the ghrelin group was higher than that in the model group, and the structure was relatively regular.

Embodiment 3

[0044] Example 3 (TUNEL staining to detect rat retinal cell apoptosis):

[0045] After dewaxing, the retinal slices were dipped twice in xylene, dehydrated with alcohol gradients, added TUNEL reaction mixture, incubated in a wet box for 2 hours, dried and incorporated into DAB chromogenic solution, and observed under a microscope that the nuclei were brownish-yellow as TUNEL If it is positive, wash the section with phosphate buffer solution to stop color development, seal the section with neutral gum, and take the image with a light microscope (200×).

[0046] Results of retinal TUNEL staining:

[0047] Such as Figure 4-6 as shown, Figure 4 Dark brown TUNEL-positive cells were occasionally seen in the retinal photoreceptor cell layer of rats in the control group. Figure 5 The number of TUNEL-positive cells in the model group increased significantly. Figure 6 After ghrelin intervention, the number of positive cells stained by TUNEL decreased.

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Abstract

The invention discloses a retina protective agent, a retina protection method and application of a retina protective agent, and belongs to the field of retinas. The retina protective agent is mainly used for improving retina injuries caused by diabetic patients and has the advantages that compared with traditional anti-VEGF drugs and hormone drugs for treating diabetic retinopathy, the protective agent is not liable to cause the problems of treatment termination and disease recurrence, is safe and non-toxic, has multiple effects of inhibiting apoptosis, inhibiting oxidative damage, delaying tissue aging and the like, and is lower in price on the market.

Description

technical field [0001] The invention relates to the field of retina, more specifically, to a retinal protective agent, a retinal protective method and the application of the protective agent. Background technique [0002] The long-term hyperglycemia state and changes in blood components in diabetic patients destroy the blood-retinal barrier, necrosis of retinal capillary pericytes, and endothelial dysfunction, resulting in the leakage of liquid components in blood vessels into the retinal tissue space, causing a series of retinal tissue damage. Changes: bleeding, edema, exudation, ischemia, etc. [0003] Although anti-VEGF drugs and hormonal drugs have been used in the treatment of diabetic retinopathy in recent years, the target of treatment is mainly for diabetic macular edema, which requires long-term continuous administration. During the treatment period, vision can be improved step by step. It is easy to cause recurrence of macular edema, and this treatment method cann...

Claims

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Application Information

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IPC IPC(8): A61K38/27A61P27/02A61P9/10A61P3/10
CPCA61K38/27A61P27/02A61P9/10A61P3/10
Inventor 白洁张永明杨帆
Owner ZHEJIANG UNIV
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