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Application of natural polymeric polysaccharide in preparation of sample treatment solution and application of natural polymeric polysaccharide in immunochromatography detection kit

A detection kit and sample processing solution technology, applied in the field of medical testing, can solve problems in lateral flow detection, formation of rejection, etc.

Pending Publication Date: 2021-11-02
BEIJING NORTH INST OF BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, some fluorescent microsphere labels with irregular shapes and sizes tend not to coat the protein uniformly during the binding process, and may not form repulsion in solution; May produce false positive results

Method used

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  • Application of natural polymeric polysaccharide in preparation of sample treatment solution and application of natural polymeric polysaccharide in immunochromatography detection kit
  • Application of natural polymeric polysaccharide in preparation of sample treatment solution and application of natural polymeric polysaccharide in immunochromatography detection kit
  • Application of natural polymeric polysaccharide in preparation of sample treatment solution and application of natural polymeric polysaccharide in immunochromatography detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 Preparation of sample treatment solutions containing different concentrations of natural polymeric polysaccharide Biosaccharide Gum-1

[0035]In the borate buffer solution with pH 8.0-8.5, add Tween 20 at a final concentration of 1% (v / v), 0.9% sodium chloride at a final concentration and proclin300 at a final concentration of 0.03%, add Biosaccharide Gum-1 , prepare the treatment solution comprising Biosaccharide Gum-1 of different working concentrations, as follows:

[0036] Treatment solution 1: boric acid buffer solution with a pH of 8.0-8.5, Tween 20 of 1% (v / v), final concentration of 0.9% sodium chloride and proclin300 of 0.03% final concentration, Biosaccharide Gum-1 concentration of 0.2 %(v / v).

[0037] Treatment solution 2: boric acid buffer solution with a pH of 8.0-8.5, Tween 20 of 1% (v / v), final concentration of 0.9% sodium chloride and proclin300 of 0.03% final concentration, Biosaccharide Gum-1 concentration of 0.4 %(v / v).

[0038] Treatment...

Embodiment 2

[0041] The preparation of embodiment 2 fluorescent immunochromatography test strips

[0042] Spray the new coronavirus antigen RBD protein and the rabbit polyclonal antibody of the new coronavirus antigen RBD protein on the nitrocellulose membrane with a gold spray machine to form parallel detection T lines and quality control C lines, and then dry; Dissolve the antigen-labeled fluorescent microspheres in the spraying buffer at a concentration of 2.3 mg / mL, and uniformly spray on the marking pad at a spraying speed of 4 μL / cm. The spraying buffer contains 0.3-0.7% BSA and 0.2- 0.3% Tween-20 concentration in 0.01-0.03M borate buffer, pH 8.0-8.5.

[0043] For the T line and C line, the spraying speed of the gold dot film machine is 1μL / cm, the T line is the new coronavirus antigen RBD protein with a concentration of 1mg / mL, and the C line is the new coronavirus antigen RBD protein with a concentration of 1mg / mL. Rabbit polyclonal antibody.

[0044] Then connect and assemble th...

Embodiment 3

[0045] Specificity determination of embodiment 3 sample treatment liquid

[0046] (1) Sample preparation: 100 μL of human serum was collected.

[0047] (2) Detection: Take the test strip, equilibrate at room temperature for 10 minutes, open the package and take out the test strip; mix 50 μL of serum and 50 μL of the sample treatment solution (various sample treatment solutions in Example 1), and add 90 μL of the mixed solution to the Put it on the sample pad of the new coronavirus protein antibody detection test strip, and after standing for 15 minutes, insert the test strip into the dry fluorescent immunoassay instrument in the direction of the arrow to determine the result. Compare the results of parallel experiments under different sample treatment solution conditions.

[0048] (3) Judgment of the result: when the T / C of the test strip is <0.12, it is judged as negative and recorded as "-"; when the T / C of the test strip is <0.12, it is judged as positive and recorded as "...

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Abstract

The invention discloses a new application of a natural polymeric polysaccharide Biosaccharide Gum <-1 >, and particularly relates to an application of the natural polymeric polysaccharide Biosaccharide Gum <-1 > in reduction of a non-specific signal of an in vitro diagnostic kit. The invention further provides a sample treatment solution containing the natural polymeric polysaccharide Biosaccharide Gum <-1 >. Biosaccharide Gum <-1 > in the treatment solution can enhance the resolubility of a fluorescent microsphere marker, and can be more easily released from a marking pad, in addition, Biosaccharide Gum <-1 > quickly forms a layer of linear polysaccharide film on an immune reaction coating film due to the film forming characteristic, the non-specific adsorption of the fluorescent microsphere marker on the coating film is reduced, further, the detection sensitivity of a fluorescence immunochromatography detection kit is improved, and meanwhile, the false positive of the kit is reduced. The sample treatment solution provided by the invention has the following advantages: the resolubility of the marker and the release capacity of the marker pad in the immunochromatography kit are improved; the non-specific detection signal of the kit is reduced, and the detection sensitivity of an immunochromatography reagent is improved; and the false positive of the kit is reduced.

Description

technical field [0001] The invention relates to the field of medical testing, in particular to a natural polysaccharide Biosaccharide Gum-1 used to prepare a sample treatment solution and its application in an immunochromatography detection kit. Background technique [0002] Immunochromatography detection kit is a technology suitable for on-site detection based on the principle of immunochromatography. Typical immunological test strips include sample pads, marker pads, coating membranes and absorbent pads. The antigen or antibody coated with nitrocellulose membrane as a solid phase carrier is used to obtain a reaction membrane, and the antibody or antigen in the sample to be tested is combined with the coating material on the reaction membrane through capillary siphoning, and colloidal gold or fluorescent markers are placed on the marker pad. Displays the response results. [0003] Nitrocellulose membrane is an important functional part of immunochromatography test strips, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/558G01N33/546G01N33/533
CPCG01N33/56983G01N33/558G01N33/54313G01N33/533G01N2333/165G01N2469/20
Inventor 段学军张旭东樊荣李玉彬常天亮侯莉莉孙蕴娴马群超
Owner BEIJING NORTH INST OF BIOLOGICAL TECH
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