Acetic acid bacteria freeze-dried powder and preparation method thereof

Abstract

The invention belongs to the field of microbial preparations, and particularly relates to acetic acid bacteria freeze-dried powder and a preparation method thereof. According to the invention, a buffer system is adopted; the activity damage of an acetic acid bacteria in the culture process is reduced through two-step pH control of a culture medium; meanwhile, the viable count of the acetic acid bacteria freeze-dried powder is further increased through the synergistic effect with a freeze-drying protective agent; industrial production of an acetic acid bacteria leavening agent with the high viable count is facilitated; and the standardization and industrialization requirements of the acetic acid bacteria are met.

Description

technical field [0001] The invention belongs to the field of microbial preparations, and in particular relates to a freeze-dried powder of acetic acid bacteria and a preparation method thereof. Background technique [0002] Acetic acid bacteria (Acetic acid bacteria, AAB) is a class of strictly aerobic Gram-negative bacteria, taxonomically belonging to αProteobacteria, Rhodospirillales, Acetoacetoaceae. Acetobacter is common in areas with warm climate and humid air, and is widely distributed in orchard soil, fruit juice, candied fruit, flowers, and the intestines of some insects. There are obvious differences in the acid resistance of different acetic acid bacteria. For example, the acid resistance of Gluconacetobacter strains is generally higher than that of Acetobacter strains; however, the strains of Saccharibacter and Asaia cannot grow on the medium containing acetic acid. At present, the understanding of the molecular mechanism of acid resistance of acetic acid bacteri...

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Problems solved by technology

[0004] In the prior art, common bacteria such as yeast and lactic acid bacteria can be prepared by industrial production to obtain corresponding freeze-dried bacteria powder, but there is no industrialization level to prepare freeze-dried bacteria powder of acetic acid bacteria with high viable count and high purity. Use acetic acid bacteria subculture, or use vinegar germ starter culture

Among them, compared with direct investment fermentation production, subculture culture greatly increases the complexity of the process, which is not conducive to the standardization of acetic acid bacteria products. Although the acetic germ starter contains acetic acid bacteria, there are fewer types of acetic acid bacteria fermented, and The number of viable bacteria of acetic acid bacteria is very low, generally less than 1×10 10 CFU / g, and the starter has not been controlled for miscellaneous bacteria, which contains a large number of bacillus, coccus, etc., it is difficult to guarantee the quality of fermented products

[0005] At this stage, the acetic acid bacteria starter with high purity and high viable count is still in the blank stage, and there is no viable count greater than 1×10 11 CFU / g of acetic acid bacteria freeze-dried starter products, this is mainly because compared with lactic acid bacteria, the acetic acid accumulated in the fermentation process of acetic acid bacteria is more lethal than lactic acid. It is extremely easy to die in the process, and it is difficult to ensure the production of acetic acid bacteria freeze-dried direct-throwing starter

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