A kind of conjugated bilirubin assay kit

A technology combining bilirubin and a kit, which is applied in the field of combined bilirubin detection in serum, achieves the effects of high precision, elimination of interference and good stability

Active Publication Date: 2022-03-25
浙江夸克生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Recently, bilirubin oxidase assay kits have gradually entered into clinical practice. However, there are few studies on the determination of conjugated bilirubin at present. Conjugated bilirubin assay kits for clinical needs are very necessary

Method used

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  • A kind of conjugated bilirubin assay kit
  • A kind of conjugated bilirubin assay kit
  • A kind of conjugated bilirubin assay kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-5

[0053] Embodiment 1-5 A kind of conjugated bilirubin assay kit

[0054] The components and dosage of the conjugated bilirubin assay kit are shown in Table 1.

[0055] Table 1

[0056]

Embodiment 6

[0065] The detection of embodiment 6 combined bilirubin assay kit

[0066] Using the Hitachi 7080 automatic biochemical analyzer, follow the steps below for detection:

[0067] (1) Basic parameters:

[0068] 1) Reaction method: two-point endpoint method; 2) Reaction temperature: 37°C; 3) Main wavelength: 450nm; 4) Secondary wavelength: 540nm; 5) Reaction time: 10min; 6) Sample / reagent: 1 / 25;

[0069] (2) Determination:

[0070] 1) Reagent preparation: mix reagent 1a and reagent 1b evenly, which is reagent 1; reagent 2 is used directly;

[0071] 2) Operation steps: as shown in Table 2;

[0072] Table 2

[0073]

[0074]

[0075] 3) Result calculation:

[0076] Bc (μmol / L) = ΔA determination × calibrator concentration / ΔA calibration.

[0077] According to the detection method of Example 6, the conjugated bilirubin test kit prepared in Examples 1-5 and Comparative Examples 1-4 is detected, and the linear range, accuracy, precision, stability and anti-interference etc....

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Abstract

The invention discloses a conjugated bilirubin assay kit, the conjugated bilirubin assay kit comprises independently packaged reagent 1 and reagent 2, wherein reagent 1 is composed of independently packaged reagent 1a and reagent 1b; the Reagent 1a comprises potassium hydrogen phthalate, sodium fluoride, disodium edetate, p-toluenesulfonic acid, N-acetylcysteine, laminarin, raffinose and surfactant; Reagent 1b comprises potassium hydrogen phthalate, ascorbic acid oxidase, laminarin, raffinose, surfactant and liquid biological preservative; said reagent 2 comprises potassium hydrogen phthalate, bilirubin oxidase, laminarin , raffinose, surfactants and liquid biopreservatives. Compared with the prior art, the detection result of the conjugated bilirubin assay kit of the present invention has high accuracy, high precision, good stability, strong anti-interference ability, good linear relationship, and meets clinical needs.

Description

technical field [0001] The invention belongs to the technical field of detection of conjugated bilirubin in serum, in particular to a detection kit for conjugated bilirubin. Background technique [0002] Bilirubin in human serum (serum) includes free bilirubin (UB), conjugated bilirubin (CB) and delta-bilirubin (deltaB), collectively referred to as total bilirubin. During the occurrence, development and recovery of hepatobiliary secretion disorders, the serum CB concentration rises and falls rapidly with the progress of the disease, so the measurement of serum CB becomes an important indicator for monitoring the hepatobiliary secretion function. [0003] At present, the detection methods of bilirubin include diazo reagent method, high performance liquid chromatography, vanadate oxidation method and bilirubin oxidase method, etc., among them, the diazo reagent method is easy to operate, but the method sensitivity and anti-hemolysis The ability is poor; while high performance...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): G01N21/31
CPCG01N21/31
Inventor王志商忆文
Owner浙江夸克生物科技有限公司