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Injectable hyaluronic acid microsphere as well as preparation method and application thereof

A technology of hyaluronic acid and microspheres, applied in the field of A61L, can solve the problems of complex preparation, uneven particle size, poor plumpness and compression elasticity of microspheres, etc., and achieves wide application prospects, simple preparation method, and biocompatibility. Good results

Pending Publication Date: 2021-11-19
上海玮沐医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, in the preparation process of microspheres, there are problems of poor plumpness and compression elasticity of the prepared microspheres, and uneven particle size. Composition, the two-step method of adding cross-linking agent later, and there will be the situation of adding cross-linking agent, there are problems such as complicated preparation and poor uniformity of microspheres

Method used

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  • Injectable hyaluronic acid microsphere as well as preparation method and application thereof
  • Injectable hyaluronic acid microsphere as well as preparation method and application thereof
  • Injectable hyaluronic acid microsphere as well as preparation method and application thereof

Examples

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Effect test

Embodiment 1

[0040] This example provides a preparation method of hyaluronic acid microspheres, including: dissolving hyaluronic acid in 2mol / L NaCl, stirring evenly, the concentration of hyaluronic acid is 8% wt, until the hyaluronic acid is completely dissolved, adjusting the pH value to about 4, Weigh EDC (0.005 mol per gram of hyaluronic acid) and dissolve it in deionized water, add it into the hyaluronic acid solution under ice bath, and stir evenly. Weigh NHS (add 0.005mol per gram of hyaluronic acid) and dissolve it in deionized water, add it to the hyaluronic acid solution, stir well, seal with plastic wrap and transfer to 4°C refrigerator for activation overnight; dissolve chitosan with 1wt% acetic acid , to obtain a chitosan solution with a chitosan mass concentration of 2wt%, mix and stir the activated hyaluronic acid and chitosan solution (amount of 5wt% hyaluronic acid solids), and get butyl acetate in addition, add cellulose acetate element (concentration 7wt%) to form a reac...

Embodiment 2

[0042] This example provides a preparation method of hyaluronic acid microspheres, including: dissolving hyaluronic acid in 2mol / L NaCl, stirring evenly, until the hyaluronic acid concentration is 8wt%, until the hyaluronic acid is completely dissolved, adjusting the pH value to about 6, Weigh EDC (0.005 mol per gram of hyaluronic acid) and dissolve it in deionized water, add it into the hyaluronic acid solution under ice bath, and stir evenly. Weigh NHS (add 0.005mol per gram of hyaluronic acid) and dissolve it in deionized water, add it to the hyaluronic acid solution, stir well, seal with plastic wrap and transfer to 4°C refrigerator for activation overnight; dissolve chitosan with 1wt% acetic acid , to obtain a chitosan solution with a chitosan mass concentration of 2wt%, mix and stir the activated hyaluronic acid and chitosan solution (amount of 5wt% hyaluronic acid solids), and get butyl acetate in addition, add cellulose acetate element (concentration 7wt%) to form a re...

Embodiment 3

[0044] This example provides a preparation method of hyaluronic acid microspheres, which includes: dissolving hyaluronic acid in 2mol / L NaCl, stirring evenly, the concentration of hyaluronic acid is 10wt%, until the hyaluronic acid is completely dissolved, and adjusting the pH value to about 4, Weigh EDC (0.005 mol per gram of hyaluronic acid) and dissolve it in deionized water, add it into the hyaluronic acid solution under ice bath, and stir evenly. Weigh NHS (add 0.005mol per gram of hyaluronic acid) and dissolve it in deionized water, add it to the hyaluronic acid solution, stir well, seal with plastic wrap and transfer to 4°C refrigerator for activation overnight; dissolve chitosan with 1wt% acetic acid , to obtain a chitosan solution with a chitosan mass concentration of 5wt%, mix and stir the activated hyaluronic acid and chitosan solution (amount of 7wt% hyaluronic acid solids), and get butyl acetate in addition, add cellulose acetate element (concentration 7wt%) to fo...

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Abstract

The invention relates to A61L, in particular to injectable hyaluronic acid microspheres as well as a preparation method and an application thereof. The method comprises the following steps: activating hyaluronic acid; preparing a water phase; and preparing the microspheres. According to the hyaluronic acid microspheres and the preparation method thereof, natural polymers such as chitosan are adopted as a cross-linking agent, the cross-linking agent and hyaluronic acid subjected to acid activation are subjected to cross-linking in an oil-water mixed system, the microspheres can be obtained, and compared with an existing method that a cross-linking agent needs to be supplemented in the microsphere cross-linking process, synthesis can be conducted in one step the preparation method is simple, and the biocompatibility is good. According to the method provided by the invention, the hyaluronic acid microspheres can be formed through one-step crosslinking, and the hyaluronic acid microspheres can be applied to the fields of medicine, beauty, skin care, cosmetics, tissue filling and repairing, biological scaffolds, ophthalmology, drug sustained release and targeted drug delivery and the like, and have a wider application prospect.

Description

technical field [0001] The present invention relates to A61L, more specifically, the present invention relates to an injectable hyaluronic acid microsphere and its preparation method and application. Background technique [0002] Hyaluronic acid has special functions of water retention, protein regulation, blood vessel wall permeability, and electrolyte diffusion. It can be used for skin water retention, transdermal absorption, and anti-aging. Among them, hyaluronic acid gel can be used for subcutaneous injection to achieve rapid moisturizing and improve elasticity. , Adjust water and oil balance, etc. However, pure hyaluronic acid will quickly degrade in the human body, affecting the role of skin filling, so it is necessary to cross-link hyaluronic acid to improve the application prospects of hyaluronic acid. [0003] The cross-linking agents currently used are generally small molecules, such as hydrazides, disulfides, aldehydes, carbodiimides and other cross-linking agent...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/20A61L27/50A61L27/60A61K8/73A61Q19/00A61K47/36A61K47/38
CPCA61L27/20A61L27/50A61L27/60A61K8/735A61K8/736A61K8/731A61Q19/00A61K47/36A61K9/0019A61K47/38A61L2400/06A61L2300/236C08L5/08C08L1/12C08L1/14
Inventor 王丽解荡
Owner 上海玮沐医疗科技有限公司
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