Preparation method and application of ternary polypeptide nanoparticles based on yak bone peptide

A nanoparticle and yak bone technology, applied in the fields of application, nanotechnology, nanotechnology, etc., to achieve the effects of low energy consumption, strong stability, and good application value

Pending Publication Date: 2021-11-26
ANHUI GUOTAI BIOTECHNOLOGY CO LTD
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, at present, there is no relevant processing technology that can assemble a variety of protein secondary structures, so that yak bone peptide can form nanoparticles and be able to load other active substances

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method and application of ternary polypeptide nanoparticles based on yak bone peptide
  • Preparation method and application of ternary polypeptide nanoparticles based on yak bone peptide
  • Preparation method and application of ternary polypeptide nanoparticles based on yak bone peptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] This embodiment provides a method for preparing ternary polypeptide nanoparticles based on yak bone peptide, the steps are as follows:

[0052] (1) Alkali adjustment and dissolution: add yak bone peptide, rice protein and casein to 100 times the mass of distilled water at a mass ratio of 1:1:0.5, and add an appropriate amount of 1mol / L sodium hydroxide aqueous solution dropwise to adjust the system The pH is 12, fully stirred to dissolve;

[0053] (2) Structure unfolding: the feed liquid obtained in step (1) was uniformly stirred for 60 minutes at a stirring rate of 600 r / min, so that the advanced spatial structure of yak bone peptide, rice protein and casein was fully developed;

[0054] (3) Co-frame combination: Add an appropriate concentration of 0.1mol / L hydrochloric acid aqueous solution dropwise to the feed liquid obtained in step (2), adjust the pH of the system to 7, and stir evenly for 60min at a stirring rate of 600r / min, so that Different proteins in the unf...

Embodiment 2

[0060] This embodiment provides a method for preparing ternary polypeptide nanoparticles based on yak bone peptide, the steps are as follows:

[0061] (1) Alkali adjustment and dissolution: add yak bone peptide, rice protein and casein to 100 times the mass of distilled water at a mass ratio of 1:1:0.75, add dropwise an appropriate amount of calcium hydroxide aqueous solution with a concentration of 0.5mol / L, and adjust The pH of the system is 11.5, fully stir to dissolve;

[0062] (2) Structure unfolding: the feed liquid obtained in step (1) was uniformly stirred for 55 minutes at a stirring rate of 650 r / min, so that the advanced spatial structure of yak bone peptide, rice protein and casein was fully developed;

[0063] (3) co-frame combination: in the feed liquid obtained in step (2), it is 0.05mol / L citric acid aqueous solution to drop an appropriate concentration, and the pH of the adjustment system is 6, and it is uniformly stirred for 55min at a stirring speed of 650r / ...

Embodiment 3

[0069] This embodiment provides a method for preparing ternary polypeptide nanoparticles based on yak bone peptide, the steps are as follows:

[0070] (1) Alkali adjustment and dissolution: add yak bone peptide, rice protein and casein to 100 times the mass of distilled water at a mass ratio of 1:1:1, and add an appropriate amount of 0.1mol / L potassium hydroxide aqueous solution dropwise to adjust The pH of the system is 12, fully stir to dissolve;

[0071] (2) Structure unfolding: the feed liquid obtained in step (1) was evenly stirred for 50 minutes at a stirring rate of 700 r / min, so that the advanced spatial structure of yak bone peptide, rice protein and casein was fully developed;

[0072] (3) Co-frame combination: Add an appropriate amount of 0.01mol / L acetic acid aqueous solution dropwise to the feed liquid obtained in step (2), adjust the overall pH to 8, and stir evenly for 50min at a stirring rate of 700r / min, so that the unfolded The interaction between different ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
particle sizeaaaaaaaaaa
molecular weightaaaaaaaaaa
molecular weightaaaaaaaaaa
Login to view more

Abstract

The invention relates to the technical field of deep processing of animal protein, and particularly relates to a preparation method and application of ternary polypeptide nanoparticles based on yak bone peptide. The preparation method of the ternary polypeptide nanoparticles based on the yak bone peptide comprises the following steps of: mixing the yak bone peptide, rice protein and casein with water to obtain a mixed system; adjusting the pH of the mixed system to be greater than or equal to 11 by adopting an alkaline solution; and after hydration, adjusting the pH of the hydrated system to be 6-8 by adopting an acidic solution, so that the protein is combined in a common frame manner. The nanoparticles with the particle size of 50-200nm can be prepared by utilizing the method, the particle size uniformity is high, and the nanoparticles have good solubility and stability; and meanwhile, according to the method, on the basis of retaining the integrity of the primary structure of the protein to the maximum extent, the secondary structure of the protein is changed through interaction, nutrition complementation is achieved through combination of different proteins, and the potential value of the yak bone peptide is improved.

Description

technical field [0001] The invention relates to the technical field of animal protein deep processing, in particular to a preparation method and application of yak bone peptide-based ternary polypeptide nanoparticles. Background technique [0002] In recent years, the rapid economic development has promoted the improvement of living standards. People's dietary requirements no longer stay at the stage of filling their stomachs. A healthy and reasonable diet has become the mainstream of people's pursuit. Protein is an important part of the daily diet, and it is necessary to ensure a certain amount of high-quality protein intake every day. However, the current supply of protein, especially the supply of high-quality protein, is still insufficient. [0003] At the same time, animal slaughter products are rich in resources, and livestock and poultry bones are not fully utilized as by-products in meat production, and a large amount of high-quality protein is wasted. Among them, ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/17A61K9/14A61K9/19A23L33/18A23L33/185A23P10/20B82Y5/00B82Y40/00A61K38/01
CPCA61K38/014A61K38/011A61K38/1709A61K9/19A61K9/14B82Y5/00B82Y40/00A23L33/185A23L33/18A23P10/20A61K2300/00
Inventor 王涛衣大龙陈正行刘怀高张恒李煦源
Owner ANHUI GUOTAI BIOTECHNOLOGY CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap