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Method for postharvest disease control, storage and preservation of cherry tomatoes by using pichia caribbica

A technology for cherry tomato and postharvest disease, which is applied in the field of cherry tomato postharvest disease control and storage and preservation, can solve the problems that the control effect needs to be improved, and achieve the effects of reducing losses, being environmentally friendly, and slowing down quality deterioration

Active Publication Date: 2021-12-03
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on the use of antagonistic yeasts to control postharvest diseases of cherry tomato, especially the control of postharvest black spot, and the control effect needs to be improved.

Method used

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  • Method for postharvest disease control, storage and preservation of cherry tomatoes by using pichia caribbica
  • Method for postharvest disease control, storage and preservation of cherry tomatoes by using pichia caribbica
  • Method for postharvest disease control, storage and preservation of cherry tomatoes by using pichia caribbica

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Pichia calibbica for postharvest black spot control of cherry tomato

[0034] 1. Test plan

[0035] Select cherry tomatoes with no disease, no mechanical damage, uniform color and equal size, rinse the surface impurities with clean water, and then soak them in 0.2% sodium hypochlorite for 3 minutes. Rinse the surface of the cherry tomatoes with running water to remove residual sodium hypochlorite. Place the cleaned cherry tomatoes in a sterilized plastic basket to dry. Make a wound of 3 mm × 3 mm (diameter × depth) on the dried cherry tomato with a sterile puncher at the fruit equator, and inject 10 μL with a concentration of 1 × 10 6 , 1×10 7 , 1×10 8 and 1×10 9 cells / mL of Pichia calibica suspension; after standing for 2 hours, re-inoculate 10 μL on the wound of cherry tomato with a concentration of 1×10 5 spores / mL of Alternaria spore suspension. Cherry tomatoes treated with sterile saline instead of yeast suspension served as the control group. After the tre...

Embodiment 2

[0040] Application of Pichia calibicus in storage and preservation of cherry tomatoes

[0041] 1. Experimental plan

[0042] Select cherry tomatoes with no disease, no mechanical damage, uniform color and similar size, without any disinfection treatment, to maintain the natural state of cherry tomatoes, directly soak the cherry tomatoes in 1.0×10 8 cells / mL of Pichia calibicus suspension. Soak the cherry tomatoes for 3 minutes, remove them, put them in a sterilized plastic basket, seal the plastic basket with plastic wrap after the cherry tomatoes dry naturally, and store them in a constant temperature and humidity incubator at 20°C (90% relative humidity) for 30 days Finally, the incidence of cherry tomatoes was counted, and samples were taken to determine the contents of soluble sugar, titratable acid, vitamin C and soluble solids in cherry tomatoes. Cherry tomatoes soaked in sterile saline were used as blank control. Each treatment was replicated three times with 15 frui...

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Abstract

The invention belongs to the technical field of biological prevention and control, and particularly relates to a method for postharvest disease control, storage and preservation of cherry tomatoes by using pichia caribbica. The method comprises the following steps: activating and culturing the yeast to obtain a culture solution, centrifuging to obtain thalli, and diluting the thalli with sterile water to obtain a yeast suspension; punching the surfaces of the cherry tomatoes, injecting the yeast suspension, and injecting an equal-volume alternaria alternata spore suspension after 2 hours to realize effective control of alternaria alternata; or directly immersing the cherry tomatoes which are not disinfected into the yeast suspension for 1-3 minutes, and naturally airing, so as to realize the prevention and treatment of the postharvest diseases of the cherry tomatoes and the storage and preservation of the cherry tomatoes. The method can effectively control the diseases and natural rot caused by the alternaria alternata after the cherry tomatoes are harvested, has no obvious adverse effect on main quality indexes after the cherry tomatoes are harvested, and can relieve the reduction of the fruit quality of the cherry tomatoes; and the method is convenient to operate, safe and environment-friendly, and has remarkable economic and social benefits.

Description

technical field [0001] The invention belongs to the field of biological control of postharvest diseases of fruits and vegetables, and in particular relates to a method for controlling postharvest diseases of cherry tomatoes and storing and keeping fresh by Pichia calibicus. Background technique [0002] Cherry tomato, scientific name Lycopersivon esculentum Mill, also known as pocket tomato, mini tomato, small tomato, etc., belongs to the genus Solanaceae and belongs to both fruit and vegetable vegetables. Cherry tomatoes are not only bright in color and beautiful in shape, but also sweet and sour in taste and rich in nutrition. It is rich in vitamins A, C and PP, trace elements, glutathione and lycopene, etc., and is listed as one of the "four major fruits" for priority promotion by the Food and Agriculture Organization of the United Nations. Cherry tomato also has special effects such as anti-cancer, anti-oxidation, lowering cholesterol, and preventing high blood pressure...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23B7/155
CPCA23B7/155A23V2002/00A23V2200/10A23V2250/76Y02A50/30
Inventor 张晓云周游张红印赵利娜周红瑶李军
Owner JIANGSU UNIV
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