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Nanofiber 3D porous aerogel and preparation method and application thereof

A technology of porous airgel and nanofibers, which can be used in pharmaceutical formulations, prostheses, and drug delivery. It can solve problems such as accelerated cell growth and unstable electrical activity, and achieve accelerated cell growth, easy processing, and easy access. The effect of biocompatibility

Active Publication Date: 2021-12-03
HUAZHONG UNIV OF SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the defects of the prior art, the object of the present invention is to provide a nanofiber 3D porous airgel and its preparation method and application, using bio-piezoelectric materials to combine 3D porous airgel is prepared by dry spinning and wet bonding electrospinning process, which can output stable current and accelerate cell growth under external stimuli, aiming to solve the problem of unstable electrical activity of existing piezoelectric materials used in tissue engineering

Method used

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  • Nanofiber 3D porous aerogel and preparation method and application thereof
  • Nanofiber 3D porous aerogel and preparation method and application thereof
  • Nanofiber 3D porous aerogel and preparation method and application thereof

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preparation example Construction

[0027] Such as figure 1 Shown, the preparation method of a kind of nanofiber 3D porous airgel provided by the present invention, it comprises the steps:

[0028] S1. Using degradable bio-piezoelectric materials as raw materials, dissolving them in a benign solvent to prepare a homogeneous spinning solution;

[0029] S2, spray the spinning solution obtained in step S1 into the coagulation bath under the action of an electric field, the degradable bio-piezoelectric material in the spinning solution is insoluble in the coagulation bath, and the coagulation bath and the benign solvent in the spinning solution are more miscible Strong, extract the benign solvent in the spinning solution to form a two-phase structure with the degradable bio-piezoelectric material as the continuous phase and the solvent as the dispersed phase, disperse evenly, and then dry to obtain nanofiber airgel;

[0030] S3, subjecting the nanofiber airgel obtained in step S2 to cross-linking treatment to gener...

Embodiment 1

[0040] 1. Dissolve PHB and chitosan by ultrasonic dispersion in isopropanol solution to prepare 8% (m / v) spinning solution, transfer the spinning solution to a 10mL syringe, the ambient temperature is 25°C ± 3°C, and the humidity is 50%±5%, the spinning voltage is 15kV, the spinning speed is 8mL / min, and the receiving distance is 15cm for spinning, and the coagulation bath adopts tert-butanol:water (v / v)=1:1 to collect nano fiber.

[0041] 2. Transfer the collected nanofibers to a beaker, and ultrasonically disperse them in an ice bath for 1 hour.

[0042] 3. The nanofiber mixture obtained by ultrasonic dispersion was frozen with liquid nitrogen, put into a freeze dryer, and freeze-dried for 48 hours to prepare a 3D porous airgel.

[0043] 4. The aerogel soaked with 1% genipin was cross-linked for 2 hours at 50° C. to obtain a nanofiber 3D porous aerogel.

[0044] In this embodiment, PHB and chitosan are used as biodegradable piezoelectric materials, which have excellent bio...

Embodiment 2

[0047] 1. Dissolve PHB and chitosan ultrasonically in isopropanol solution to prepare a 10% (m / v) spinning solution, transfer the spinning solution to a 10mL syringe, the ambient temperature is 25°C ± 3°C, and the humidity is 50%±5%, the spinning voltage is 15kV, the spinning speed is 6mL / min, and the receiving distance is 15cm for spinning, and the coagulation bath adopts tert-butanol:water (v / v)=1:1 to collect nano fiber.

[0048] 2. Transfer the collected nanofibers to a beaker, and ultrasonically disperse them in an ice bath for 1 hour.

[0049] 3. The nanofiber mixture obtained by ultrasonic dispersion was frozen with liquid nitrogen, put into a freeze dryer, and freeze-dried for 48 hours to prepare a 3D porous airgel.

[0050] 4. The aerogel soaked with 1% genipin was cross-linked for 2 hours at 30° C. to obtain a nanofiber 3D porous aerogel.

[0051] The airgel prepared in this example has a 3D structure of nanofibers, and the fibers are interlaced to form a porous ne...

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Abstract

The invention belongs to the technical field of tissue engineering scaffolds, and particularly discloses nanofiber 3D porous aerogel and a preparation method and application thereof. The method comprises the steps of: S1, dissolving a degradable biological piezoelectric material in a solvent, and preparing a spinning solution; S2, spraying the spinning solution into a coagulating bath under the action of an electric field, uniformly dispersing, and then drying to obtain nanofiber aerogel; and S3, carrying out crosslinking treatment on the nanofiber aerogel, so as to obtain the nanofiber 3D porous aerogel. Compared with the prior art, the method has the advantages that the preparation process is simple and easy to implement, and the surface of the prepared 3D nanofiber is of a bitter gourd-like epidermis rough structure; the aerogel structure is internally rich in a large number of pores, is similar to a cell growth microenvironment, and is non-toxic and excellent in biocompatibility; and the special piezoelectric effect of the used piezoelectric material can convert external mechanical stimulation into electric energy, and the micro electric field of the human body is combined to stimulate and accelerate cell growth by generating micro current.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering scaffolds, and more specifically relates to a nanofiber 3D porous airgel and its preparation method and application. Background technique [0002] In tissue engineering, it is necessary to implant artificial tissues into damaged tissues or organs. In order to proliferate and differentiate seed cells, it is necessary to provide a cell scaffold composed of biomaterials. The implanted scaffold material has a geometry that conforms to the damaged tissue or organ. The shape is equivalent to artificial extracellular matrix. Tissue engineering scaffold materials include tissue scaffold materials for bone, cartilage, blood vessels, nerves, skin and artificial organs, such as liver, spleen, kidney, bladder, etc. [0003] Current research has found that wound-induced currents may stimulate tissue growth, a phenomenon known as galvanotaxis. Therefore, simulating endogenous currents at the wound ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/56A61L27/58C08J9/40C08J9/36C08L5/08
CPCA61L27/56A61L27/58C08J9/40C08J9/36A61L2400/12C08J2305/08
Inventor 李阳孙双王云明周华民黄志高张云周何乐子李德群
Owner HUAZHONG UNIV OF SCI & TECH
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