A methanotroph with denitrification function and hypoxia resistance and its application

A methane-oxidizing bacteria and stress-resistant technology, applied in the field of microorganisms, can solve the problems that aerobic methane-oxidizing bacteria cannot survive for a long time, and achieve the effects of contributing to sustainable development, reducing accidents, and stabilizing performance

Active Publication Date: 2022-02-11
GUANGDONG INST OF ECO ENVIRONMENT & SOIL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these aerobic methanotrophs cannot survive long-term under hypoxic conditions

Method used

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  • A methanotroph with denitrification function and hypoxia resistance and its application
  • A methanotroph with denitrification function and hypoxia resistance and its application
  • A methanotroph with denitrification function and hypoxia resistance and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Methanotrophs Methylobacter Isolation and identification of sp. YRD-M2.

[0042] 1. Enrichment culture of methanotrophs:

[0043] Pre-incubation culture was carried out on the wetland soil of the Yellow River Delta. Take 10 g of fresh soil in a 125 mL vial and seal it with butyl rubber and an aluminum cap. Add about 5% methane (6 mL) as a substrate and culture statically in a dark room at 30 °C. During this period, 0.2 mL of gas in the bottle was taken with a gas sampling needle, and the methane concentration was detected by high-performance gas chromatography (Agilent 7820A, USA). The pre-incubated soil was used as the inoculum for methane oxidation enrichment culture. Inoculate 2.5 g of pre-incubated soil into 25 mL of nitrate mineral salt (NMS) medium. The preparation process of NMS medium is as follows: Weigh 0.2 g MgSO with an electronic balance 4 ·7H 2 O, 0.14 g CaCl 2 ·6H 2 O and 1.0 g KNO 3 , and use a syringe to take 50 mL of phosphate buffer (5.44 ...

Embodiment 2

[0055] Methanotrophs Methylobacter The effect of sp. YRD-M2 utilizing methane and nitrate under different methane concentration, air volume and nitrate concentration.

[0056] 1) Preparation of NMS medium:

[0057] Weigh 0.2 g MgSO with an electronic balance 4 ·7H 2 O, 0.14 g CaCl 2 ·6H 2 O and 1.0 g KNO 3 , and use a syringe to take 50 mL of phosphate buffer (5.44 g / L KH 2 PO 4 , 5.68 g / L Na 2 HPO 4 ) and 2 mL trace element solution (1.0 g Na per liter 2 -EDTA, 2.0 g FeSO 4 ·7H 2 O, 0.8 g ZnSO 4 ·7H 2 O, 0.03 g MnCl 2 4H 2 O, 0.03g H 3 BO 3 , 0.2 g CoCl 2 ·6H 2 O, 0.6 g CuCl 2 2H 2 O, 0.02 g NiCl 2 ·6H 2 O and 0.05 g Na 2 MoO 4 2H 2 O), add it to deionized water, mix well and adjust the volume to 1 L, add sodium hydroxide to adjust the pH value to 7.0, take 25 ml of the medium and put it into a 125 mL vial, seal it with a butyl rubber stopper, and then use aluminum Cover and seal, and sterilize at 121°C for 20 min to obtain culture medium.

[005...

Embodiment 3

[0065] Methanotrophs Methylobacter Methane and nitrate utilization effects of sp. YRD-M2 after prolonged hypoxic culture.

[0066] 1) Preparation of NMS medium under hypoxic conditions:

[0067] Weigh 0.2 g MgSO with an electronic balance 4 ·7H 2 O, 0.14 g CaCl 2 ·6H 2 O and 0.23 g KNO 3 , and use a syringe to take 50 mL of phosphate buffer (5.44 g / L KH 2 PO 4 , 5.68 g / L Na 2 HPO 4 ) and 2 mL trace element solution (1.0 g Na per liter 2 -EDTA, 2.0 g FeSO 4 ·7H 2 O, 0.8 g ZnSO 4 ·7H 2 O, 0.03 g MnCl 2 4H 2 O, 0.03 g H 3 BO 3 , 0.2 g CoCl 2 ·6H 2 O, 0.6 g CuCl 2 2H 2 O, 0.02 g NiCl 2 ·6H 2 O and 0.05 gNa 2 MoO 4 2H 2 O), add it to deionized water, mix well and adjust the volume to 1 L, add sodium hydroxide to adjust the pH value to 7.0, take 25ml of the medium and put it into a 125mL vial, seal it with a butyl rubber stopper, and then cover it with an aluminum cap seal. Use an intelligent anaerobic preparation instrument (Beijing Asp Technology Co.,...

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Abstract

The invention provides a methane oxidizing bacterium with denitrification function and anoxic stress resistance and its application 。 The methane-oxidizing bacteria can efficiently degrade nitrate under different environmental conditions and carry out the methane oxidation process at the same time. It has stress resistance to anoxic environment, simple cultivation, low cost, strong adaptability and good stability of the strain, and has a certain role in the field of sewage treatment. Wide application prospects. The strain uses methane and nitrate as carbon and nitrogen sources to form bacterial protein, which can be used as feed, etc., to realize the transformation of pollutants into higher value-added products, and has the advantage of comprehensive pollution control.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a methane-oxidizing bacterium with denitrification function and anoxic stress resistance and its application. Background technique [0002] Due to climate warming, the atmospheric circulation is affected, making the weather unstable in some areas, high temperature and heat waves, heavy rain and floods, cold waves and snow disasters occur frequently. In 2020, scientists detected accelerated melting of sea ice in the Arctic Ocean and large cracks in Antarctic glaciers. Since the industrial revolution, the concentration of methane in the atmosphere has increased rapidly, at a rate of 1.0% to 1.2% per year, and the impact of methane on global climate change is increasing day by day. Although the content of methane in the atmosphere is much lower than that of carbon dioxide, its greenhouse effect is 26.5 times that of carbon dioxide, and it has a higher global warming potentia...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/32C02F101/16
CPCC12N1/20C02F3/34C02F2101/32C02F2101/163
Inventor 刘芳华郝钦钦谢章彰汤佳
Owner GUANGDONG INST OF ECO ENVIRONMENT & SOIL SCI
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