Agricultural microbial agent and preparation method thereof

A technology of microbial inoculum and Lactobacillus plantarum, applied in the field of agricultural microbial inoculum and preparation, can solve problems such as unsatisfactory effect, ineffectiveness, poor synergy ability, etc., and achieves improved crop yield and quality, short production cycle, The effect of promoting synthesis

Inactive Publication Date: 2021-12-10
玉林市桂芭农业发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, the single microbial agent has a single effect and the effect is not ideal; some compound microbial agent

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1: a kind of preparation method of polypeptide agricultural microbial inoculum, the steps are as follows:

[0032] (1) Preparation of seed liquid: Inoculate the nitrogen-fixing rhodobacter bacteria cultured on RCVBN slant medium, Lactobacillus plantarum cultured on MRS slant medium, and powdery Pichia pastoris cultured on YED slant medium into the seed medium respectively. Cultivate at 28°C for 96 hours to obtain seed liquids of Rhodobacter nitrogen-fixing bacteria, Lactobacillus plantarum, and Pichia powderylum respectively. 3×10 10 CFU / mL, 2×10 10 CFU / mL, 1×10 10 CFU / mL;

[0033] (2) Preparation of pretreatment mixture: in parts by weight, add 40 parts of soybean meal powder and 20 parts of sea fish to adjust the moisture content to 70wt%, let stand at 28°C for 12 hours, then place at 115°C Cook for 20 minutes, after cooling, add 20 parts of molasses powder and 15 parts of maltitol and mix evenly to obtain a pretreatment mixture, which is set aside;

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Embodiment 2

[0036] Embodiment 2: a kind of preparation method of polypeptide agricultural microbial inoculum, the steps are as follows:

[0037] (1) Preparation of seed liquid: Inoculate the nitrogen-fixing rhodobacter bacteria cultured on RCVBN slant medium, Lactobacillus plantarum cultured on MRS slant medium, and powdery Pichia pastoris cultured on YED slant medium into the seed medium respectively. Cultivate at 30°C for 72 hours to obtain the seed liquids of Rhodobacter nitrogen-fixing bacteria, Lactobacillus plantarum and Pichia powderylum respectively. 2×10 10 CFU / mL, 1×10 10 CFU / mL, 1×10 10 CFU / mL.

[0038] (2) Preparation of pretreatment mixture: in parts by weight, add 50 parts of soybean meal powder and 15 parts of sea fish to adjust the water content to 80wt%, let stand at 25°C for 24 hours, then place at 121°C Cook for 20 minutes, after cooling, add 10 parts of molasses powder and 30 parts of maltitol and mix evenly to obtain a pretreatment mixture, which is set aside;

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PUM

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Abstract

The invention relates to an agricultural microbial agent containing rhodobacter azotoformans, lactobacillus plantarum, pichia farinosa, amino acid and maltitol as well as a preparation method and application of the agricultural microbial agent. According to the invention, marine trash fish and soybean meal which are rich in protein are used as main raw materials, maltitol and molasses powder are used as auxiliary materials, and after reasonable compounding, different proteins in the raw materials are decomposed under specific fermentation conditions, so the agricultural microbial agent which takes various thalli, amino acid, polysaccharides and the like as efficient components is prepared. The agricultural microbial agent can effectively improve the micro-ecological environment of soil and promote the conversion of organic matters in the soil, and various bacteriostatic active substances generated by beneficial microorganisms can enhance the ability of crops to resist various diseases, reduce the occurrence of soil-borne diseases and promote the robust growth of plants. The agricultural microbial agent can effectively promote the formation of a soil aggregate structure, enables the soil to become loose and fertile, and is beneficial to cultivation and growth and development of crop roots. The agricultural microbial agent contains rich organic matters, and can activate plough layer soil, improve the water retention capacity of the soil, enhance the adsorption effect of the organic matters, absorb and accommodate various nutrient substances, effectively avoid nutrient loss, improve the fertilizer utilization rate and enhance the fertilizer retention and supply capacity and buffer capacity of the soil.

Description

technical field [0001] The invention belongs to the technical field of microbial application, and in particular relates to an agricultural microbial bacterial agent and a preparation method. Background technique [0002] At present, soils all over the world are facing problems such as deterioration of soil properties, soil acidification, salinization, and destruction of soil microbial diversity, which directly lead to soil barrenness and soil failure, which affect the sustainable development of agriculture. Under the situation of such poor soil ecology, how to improve soil, enhance soil fertility, improve soil ecological environment, and improve crop yield and quality has become the top priority of current agricultural work. [0003] Microorganisms can promote the release and chelation of trace elements in the soil through the exchange of oxygen and carbon dioxide and the secretion of organic acids and other acidic substances during the metabolic process, effectively break t...

Claims

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Application Information

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IPC IPC(8): C05F11/08C05G3/80C12N1/20C12N1/16C12R1/01C12R1/25C12R1/84
CPCC05F11/08C05G3/80C12N1/20C12N1/16
Inventor 黄文韦茂春吕秋陵
Owner 玉林市桂芭农业发展有限公司
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