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Optimization method of threonine production and extraction process

An optimization method and extraction process technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, organic chemical methods, etc., can solve the problem of inability to maintain and improve the oxygen utilization rate of bacteria and the deterioration of oxygen absorption and utilization of bacteria and other issues to achieve the effect of improving intake efficiency, increasing production efficiency, and increasing output

Pending Publication Date: 2021-12-10
HULUNBEIER NORTHEAST FUFENG BIOTECHNOLOGIES CO LTD
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  • Claims
  • Application Information

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Problems solved by technology

It is simple and effective to maintain stable dissolved oxygen during the entire fermentation process by adjusting the rotation speed and ventilation volume. However, as the fermentation time increases, the dissolved oxygen environment and the absorption and utilization of oxygen by bacteria become worse, and conventional means such as stirring and ventilation cannot be used. To maintain and improve the oxygen utilization rate of bacteria

Method used

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  • Optimization method of threonine production and extraction process
  • Optimization method of threonine production and extraction process

Examples

Experimental program
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Effect test

Embodiment 1

[0031] Threonine fermentation production process:

[0032] Step 1) Prepare fermentation medium: 60g / L sucrose, 30g / L glucose, 20g / L corn steep liquor, 5g / L ammonium sulfate, 0.5g / L potassium dihydrogen phosphate, 0.5g / L dipotassium hydrogen phosphate, heptahydrate Magnesium sulfate 0.1g / L, ferrous sulfate heptahydrate 10mg / L, manganese sulfate monohydrate 10mg / L, V B1 2mg / L, V H 50μg / L. Step 2) Fermentation: Put the seed liquid of the L-threonine-producing strain (Trfc as an example) into a fermenter containing a fermentation medium at an inoculation amount of 1.5%, and ferment at an inoculation density of OD 600The temperature is 0.4, the temperature is 36°C, the stirring speed is 300rpm, the dissolved oxygen is controlled to 20% by ventilation and stirring, and the foam is used to defoam. The details are as follows: 1) Control the sugar content to 3% by adding 50% sucrose solution (50g of sucrose added with water to 100ml is 50% solution) until the end of fermentation; ...

Embodiment 2

[0037] Threonine extraction process:

[0038] 1) Filtration: The fermentation broth is first centrifuged at 4000rpm for 5 minutes by a disc centrifuge to collect the upper liquid and sediment; the upper liquid is filtered through a ceramic membrane (10,000 Da molecular weight cut-off), the filtrate is collected, and the filtrate is centrifuged on a decanter Machine separation, centrifugation speed is 5000rpm, centrifugation time is 3min, collect supernatant; then filter through ultrafiltration membrane, collect permeate, ultrafiltration membrane molecular weight cut-off is 300Da;

[0039] 2) Concentration and crystallization: Concentrate the permeate through the evaporator to 1 / 3 of the volume of the original solution, and crystallize it in an intermittent single-effect concentration crystallization pot. When the feed liquid in the pot is concentrated to 27-30 Baume degree, start adding crystal seeds. The crystallization process is controlled at a temperature of about 65°C and...

Embodiment 3

[0047] On the basis of Comparative Example 1, improvements were made to increase the fermentation yield of threonine.

[0048] 1. Set the concentration of mannitol in the mixed aqueous solution to 0, 5, 10, 15, 20, 25, 30, the unit is g / L, such as figure 1 As shown, with the addition of mannitol concentration, the content of threonine in the fermentation broth also increased. When the concentration of mannitol reached 15g / L, the content of threonine was close to the peak value. Continue to increase the concentration of mannitol. Yield has little effect. Therefore, the concentration of mannitol was selected to be 15g / L.

[0049] 2. Select the added concentration of mannitol as 15g / L, and set the concentration of α-ketoglutaric acid as 0, 5, 10, 15, 20, 25, 30, the unit is g / L, such as figure 2 As shown, the addition of low concentration (0-10g / L) has little effect on the content of threonine in the fermentation broth. When the concentration of α-ketoglutaric acid increases t...

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Abstract

The invention belongs to the technical field of amino acid production, and discloses an optimization method of a threonine production and extraction process. The method comprises a production fermentation process and a separation and extraction process. A threonine product produced by the process flow is stable in quality, and the main indexes of the threonine product are obviously superior to requirements of feed-grade L-threonine (GB / T21979-2008).

Description

technical field [0001] The invention belongs to the technical field of amino acid production, and in particular relates to an optimization method for the production and extraction process of threonine. Background technique [0002] Threonine belongs to the aliphatic amino acid and is one of the eight essential amino acids. L-threonine plays an important role in promoting human growth and maintaining normal physiological functions, so it is widely used in food and drug fields. Due to the large production volume of food and the influence of a wide range of distribution areas, the nutrients in food are easily damaged and cause some losses, so nutritional additives play an important role in the food industry. Among them, threonine, as an amino acid additive, can effectively enrich nutrients and improve the antioxidant capacity of food. At the same time, the co-heating of threonine and glucose also plays a good role in flavor enhancement. Secondly, threonine is also an essenti...

Claims

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Application Information

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IPC IPC(8): C12P13/08C12N1/20C12N1/38C07C227/40C07C229/22C12R1/19
CPCC12P13/08C12N1/20C12N1/38C07C227/40C07B2200/07C07C229/22
Inventor 刘元涛赵兰坤王小平罗玮边建军尤学波吴学雷
Owner HULUNBEIER NORTHEAST FUFENG BIOTECHNOLOGIES CO LTD
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