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Method for enriching flavonoid, terpenoid and/or stilbene compounds in peanut plants

A technology for stilbene compounds and peanut plants, which is applied in the field of natural product preparation, can solve the problems of weak extraction and production capacity, insufficient development and utilization of peanut plants, and low degree of actual utilization, etc., so as to improve biological and physiological functions. , easy to implement, and the effect of improving the content and safety of active ingredients

Active Publication Date: 2021-12-14
SHANDONG PEANUT RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the medicinal value of peanut stems and leaves has gradually been paid attention to, the current industrial extraction and production capacity of peanut stems and leaves active ingredients is not strong, and a large number of groundnut stems and leaves are still burned as waste, and the actual utilization degree is not high.
[0006] In summary, the current peanut plants have not been fully developed and utilized

Method used

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  • Method for enriching flavonoid, terpenoid and/or stilbene compounds in peanut plants
  • Method for enriching flavonoid, terpenoid and/or stilbene compounds in peanut plants
  • Method for enriching flavonoid, terpenoid and/or stilbene compounds in peanut plants

Examples

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Effect test

Embodiment 1

[0110] A method for enriching flavonoid compounds in peanut shells, the specific steps are as follows:

[0111] (1) After washing and drying the peanut pod shells, ultrafinely pulverize and pass through a 100-mesh sieve;

[0112] (2) Add water to the ultra-finely pulverized peanut pod shell powder at a solid-to-liquid ratio of 1:20 (g / mL), shake and extract for 60 minutes at 30°C and 180r / min, retain the precipitate after centrifugal suction, and repeat This operation is performed 3 times;

[0113] (3) Add 10% ethanol aqueous solution to the precipitate obtained in step (2) at a solid-to-liquid ratio of 1:20 (g / mL), shake and extract for 60 minutes at 30°C and 180r / min, and retain the precipitate after centrifugal suction filtration. Repeat this operation 3 times;

[0114] (4) Add 40% ethanol aqueous solution to the precipitate obtained in step (3) at a solid-to-liquid ratio of 1:20 (g / mL), shake and extract for 60 minutes at 30°C and 180r / min, and retain the supernatant aft...

Embodiment 2

[0165] A method for enriching stilbene compounds in peanut roots, the specific steps are as follows:

[0166] (1) Wash and dry the peanut root and then ultrafinely pulverize it and pass through a 100-mesh sieve;

[0167] (2) Add water to the ultra-finely pulverized peanut root powder according to the solid-liquid ratio of 1:20 (g / mL), shake and extract for 40 minutes at 30°C and 180r / min, retain the precipitate after centrifugal suction filtration, repeat the process Operate 4 times;

[0168] (3) Add 20% ethanol aqueous solution to the precipitate obtained in step (2) at a solid-to-liquid ratio of 1:20 (g / mL), shake and extract for 40 minutes at 30°C and 180r / min, and retain the precipitate after centrifugal suction filtration. Repeat this operation 4 times;

[0169] (4) Add 60% ethanol aqueous solution to the precipitate obtained in step (3) at a solid-to-liquid ratio of 1:20 (g / mL), shake and extract for 60 minutes at 30°C and 180r / min, and retain the supernatant after cen...

Embodiment 3

[0186] A method for enriching flavonoids and terpenoids in peanut stems and leaves, the specific steps are as follows:

[0187] (1) Peanut stems and leaves are cleaned and dried, then pulverized and passed through a 30-mesh sieve;

[0188] (2) Add 30% ethanol aqueous solution to the crushed peanut stem and leaf powder according to the solid-to-liquid ratio of 1:20 (g / mL), shake and extract for 30 minutes at 30°C and 180r / min, and retain the supernatant after centrifugal suction Liquor and precipitation, repeat this operation 5 times to obtain leaching supernatant A;

[0189] (3) The supernatant A obtained in step (2) was concentrated to 15% of the original volume by rotary evaporation, and then freeze-dried to obtain powder A;

[0190] (4) Add 50% ethanol aqueous solution to the precipitate obtained in step (2) at a solid-to-liquid ratio of 1:20 (g / mL), shake and extract for 30 minutes at 30°C and 180r / min, and retain the supernatant after centrifugal suction solution, repea...

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Abstract

The invention discloses a method for enriching flavonoid, terpenoid and / or stilbene compounds in peanut plants, and belongs to the technical field of natural product preparation. The preparation method comprises the following steps: by taking peanut plants as raw materials, carrying out superfine grinding, then removing impurities, adding an ethanol aqueous solution into the peanut plants subjected to impurity removal, extracting, concentrating and freeze-drying supernate, further carrying out redissolving treatment on the obtained powder, and purifying, concentrating and freeze-drying the obtained peanut plant active component enriched product by macroporous resin, obtaining and packaging high-purity flavonoid, terpenoid and / or stilbene compound powder, and obtaining a finished product. According to the method, the purity of effective components in the peanut plant extract product is efficiently improved, the process is simple, operability is high, cost is low, the method is efficient and environmentally friendly, industrial production is easy, and the prepared product is high in content of effective active components and high in safety.

Description

technical field [0001] The invention belongs to the technical field of natural product preparation, and in particular relates to a method for enriching flavonoids, terpenes and / or stilbenes in peanut plants. Background technique [0002] Peanut plants are used as peanut waste, except for a few used as feed, most of them are discarded or burned, resulting in waste of resources and environmental pollution. Peanut plants contain a variety of active ingredients, among which, peanut shells are rich in flavonoid compounds such as luteolin and eriodictyol, as well as some active ingredients such as phenols, saponins, and sterols; peanut roots have biological activity stilbene compounds; peanut stems and leaves are rich in flavonoids, terpenes, phenols, alkaloids, anthraquinones (such as aloin, chrysophanol, etc.), phenylpropanoids (such as coumarin, lignans, etc.) , steroids (such as phytosterols, etc.), stilbenes (such as resveratrol, etc.) compounds and other active ingredients....

Claims

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Application Information

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IPC IPC(8): A61K36/48A23K20/121A23K20/111A23K20/105A23K10/30A23L33/105
CPCA61K36/48A23K20/121A23K20/111A23K20/105A23K10/30A23L33/105A61K2236/333A61K2236/53A61K2236/51A61K2236/15A23V2002/00A23V2200/30A23V2250/21A23V2250/2116
Inventor 毕洁于丽娜杨伟强石程仁王明清江晨齐宏涛宋昱徐同成彭娅萍孙杰龚魁杰谢宏峰孙小双
Owner SHANDONG PEANUT RES INST