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Application of serratia marcescens strain in decoloration and degradation of vat blue RSN anthraquinone dye

A technology for Serratia marcescens and anthraquinone dyes, which is applied in the field of microorganisms, can solve the problems that the biodegradation process of anthraquinone dyes is poorly understood, and achieves the effects of good decolorization effect, strong practicability and high degradation rate.

Pending Publication Date: 2021-12-17
XI'AN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, little is known about the biodegradation process of anthraquinone dyes

Method used

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  • Application of serratia marcescens strain in decoloration and degradation of vat blue RSN anthraquinone dye
  • Application of serratia marcescens strain in decoloration and degradation of vat blue RSN anthraquinone dye
  • Application of serratia marcescens strain in decoloration and degradation of vat blue RSN anthraquinone dye

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment one: the acquisition of Serratia viscosei

[0038] 1. Obtaining the strain

[0039] (1) Sludge samples were collected from the collection well and oxidation pond of the wastewater treatment station of Xianyang Jihuaxin Sanzero Printing and Dyeing Co., Ltd. Take 5.0g (or 10g) of sludge and put it into an appropriate amount of sterile water, fully oscillate the sludge with glass beads to obtain a mother solution for inoculating microorganisms. Take 1mL of the mother liquor and inoculate it into LB medium, enrich and culture it in a shaker at 30°C and 180rpm for 3 days, and transfer it to fresh ISM+ Vat blue RSN (20mg / L) and ISM+glucose at 1% inoculum size (v / v) (0.1g / L)+ Vat blue RSN (20mg / L) in two kinds of culture medium, adopt gradient concentration pressure domestication method, subculture continuously, increase dye concentration (20,40,60,80,100,120,150 successively) , 180, 200, 220 and 250mg / L). The decolorization rate was detected by ultraviolet spect...

Embodiment 2

[0042] Embodiment two: the identification of Serratia viscosei

[0043] (1) Morphological observation

[0044] Observe the plate culture colony morphology of the strains, such as figure 2 shown. The bacteria can grow in large quantities on the beef extract peptone agar medium plate at 25-35°C for 1 day, and the colonies on the LB plate are light yellow colonies with neat, opaque edges, raised colonies and smooth surfaces.

[0045] Observe the morphology of the bacteria under a light microscope, such as image 3 As shown, the bacteria are rod-shaped. Gram-stain the strain, which is Gram-negative, using Gram staining solution.

[0046] (2) Molecular identification

[0047] The WY31 bacterial liquid pretreated by hot alkali method was used as a template, and the general primer 27F / 1492R was used for PCR amplification to obtain a fragment of about 1,500bp, such as Figure 4 . Entrust the company to sequence the PCR product of the sample with the correct target band to obta...

Embodiment 3

[0050] Example 3: Application of Serratia marcescens WY31 in the decolorization and degradation of anthraquinone dye vat blue RSN

[0051] (1) Inoculate the seed liquid of Serratia marcescens WY31 into the medium containing VB RSN anthraquinone dye;

[0052] (2) Add nutrient source and mix well;

[0053] (3) At a certain initial inoculation amount OD 600 , NH 4 Cl concentration, glucose concentration, pH value, temperature and Mn 2+ Cultivate under concentration conditions for decolorization and degradation.

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Abstract

The invention belongs to the technical field of microorganisms, and particularly relates to a serratia marcescens strain with the strain name of Serratia marcescens WY31. The preservation number of the strain is CCTCC AB 2019319. The 16S rRNA sequence of the serratia marcescens strain is as shown in SEQ ID N0.1. The novel strain provided by the invention provides application of serratia marcescens in decoloration and degradation of anthraquinone dye vat blue RSN, provides optimal condition parameters of decoloration and degradation, and provides a green and environment-friendly application method of microbial decoloration and degradation of anthraquinone dye vat blue RSN. Under a determined optimal condition, the strain has high degradation rate on anthraquinone dye vat blue RSN.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a strain of Serratia marcescens and its application in the decolorization and degradation of anthraquinone dye vat blue RSN. Background technique [0002] In my country, about 8-15 tons of dye wastewater are discharged into the water environment every year. [1] , dyes are usually toxic, if ingested orally or inhaled, and contact with skin or eyes, can cause skin sensitization and carcinogenicity. At the same time, when dye wastewater enters the ecosystem, it will interfere with light transmission, inhibit the photosynthesis of aquatic organisms, and destroy the microbial community structure of the ecosystem. Most dyes are biologically toxic, teratogenic and carcinogenic, and are extremely difficult to degrade in the natural environment. Some disperse dyes of anthraquinone dyes (some disperse dyes with anthraquinone structure) often contain one or more Primary amine...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C12N1/20C02F101/30C02F101/34C12R1/43
CPCC02F3/34C12N1/20C02F2101/308C02F2101/34
Inventor 李海红王洋宾齐
Owner XI'AN POLYTECHNIC UNIVERSITY