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Phosphate solubilizing bacterium 3-1 and application thereof in phosphate dissolving

A technology of insoluble phosphate and phosphate solubilizing bacteria, applied in the field of phosphate solubilizing bacteria, can solve the problems of acidification fertility, water pollution, soil compaction, etc., and achieve the effect of reducing the use of chemical fertilizers, increasing the content of available phosphorus, and alleviating soil compaction

Active Publication Date: 2021-12-17
CHINA THREE GORGES UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Long-term input of phosphate fertilizers causes soil compaction, acidification and fertility decline, leading to serious water pollution and soil pollution

Method used

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  • Phosphate solubilizing bacterium 3-1 and application thereof in phosphate dissolving
  • Phosphate solubilizing bacterium 3-1 and application thereof in phosphate dissolving
  • Phosphate solubilizing bacterium 3-1 and application thereof in phosphate dissolving

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 Isolation and Screening of Phosphobacteria (Enterobacter ludwigii strain 3-1) 3-1

[0041] 1. Primary screening of strains

[0042] Soil samples were collected from the phosphate mine in Yichang City, Hubei Province (N: 31°13’55”N, E: 111°15’36”), put them into a sterile bag and brought them back to the laboratory, and stored them at 4°C. Weigh 10 g of fresh soil samples and add them to 90 mL of sterile saline, shake at 100 r / min for 15 min, and place them in an incubator at 28°C for 24 h. After enrichment, the supernatant was diluted to 10 -3 、10 -4 、10 -5 For three gradients, 350 μL of each dilution was applied to the inorganic phosphorus screening solid medium, and each group had 3 parallels. Place it upside down in a 28°C incubator for 4-5 days, and observe the transparent circle. Pick the colonies with transparent circles and repeatedly streak and purify them. After purification, pick a single colony and transfer it to the slant of beef extract pepto...

Embodiment 2

[0045] Determination of Phosphorus Solubilizing Inorganic Phosphorus Ability

[0046] Prepare 100mL solid inorganic phosphorus screening medium with deionized water, sterilize at 121°C for 30 minutes, pour it into a disposable plate, and cool it for later use. Inoculate the phosphobacteria 3-1 preserved in Example 1 into the beef extract peptone liquid medium, culture on a shaker at 28°C and 200 r / min for 24 hours, take 10 μL of the seed liquid of phosphobacteria 3-1 and inoculate it with the spot inoculation method to the solid inorganic phosphorus screening medium plate, and cultured statically in a 28°C incubator. After 7d, observe and measure the bacterial circle and the phosphorus-dissolving circle size of its bacterial colony, and calculate the phosphorus-dissolving index: (phosphate-dissolving circle-bacterial circle) / bacterial circle.

[0047] Tricalcium phosphate, iron phosphate, and aluminum phosphate were used as the only phosphorus source to prepare liquid inorgan...

Embodiment 3

[0057] Determination of ability to dissolve organic phosphorus (lecithin is organic phosphorus)

[0058] Inoculate the phosphate solubilizing bacteria 3-1 preserved in Example 1 into beef extract peptone liquid medium, culture on a shaker at 28°C and 200r / min for 24h, take 1mL seed solution and centrifuge at 8000r / min for 5min, remove the supernatant, and Add 1mL of sterile water to the tube and beat until uniform, add Montina medium, 28°C, 200r / min shaking table fermentation culture for 5 days. Take 2mL fermentation broth and centrifuge at 12000r / min for 10min, and measure the soluble phosphorus content in the supernatant by molybdenum-antimony anti-colorimetry.

[0059] Table 3 Decomposition ability of phosphate solubilizing bacteria to lecithin

[0060]

[0061] The results showed that the ability of Enterobacter ludwigii strain 3-1 to decompose lecithin was 2.16mg / L.

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Abstract

The invention relates to the technical field of agriculture and microorganisms and discloses a phosphate solubilizing bacterium 3-1 with high salt resistance and heavy metal cadmium resistance and application of the phosphate solubilizing bacterium 3-1. The bacterial strain is Enterobacter ludwigii strain 3-1 which is preserved in China Center for Type Culture Collection in Wuhan University on June 18, 2021, and the preservation number of the bacterial strain is CCTCC M 2021734. The phosphate solubilizing bacterium (Enterobacter ludwigii strain 3-1) 3-1 disclosed by the invention has the effects of solubilizing phosphorus, fixing nitrogen, producing plant growth hormones and promoting plant growth, and can be applied to soil with high salinity and high heavy metal concentration. When the phosphate solubilizing bacterium 3-1 is applied to soil and plant seedlings, the content of available phosphorus in the soil can be remarkably increased, the plant height, wet weight, dry weight and rhizome length of plants are greatly increased, and the phosphate solubilizing bacterium 3-1 has an important significance in agricultural production and sustainable development.

Description

technical field [0001] The invention relates to the fields of agricultural planting and microbiology, in particular to a phosphate solubilizing bacterium and its application. Background technique [0002] Phosphorus is an essential element for plant growth and development. It is not only a component of important chemical substances in plants, but also has the ability to improve plant stress resistance and adapt to the external environment. If there is a lack of phosphorus in the soil, it will seriously affect the various life activities of plants. So phosphorus is the most important nutrient limiting plant growth besides nitrogen. According to reports, about 74% of the cultivated land in my country is deficient in phosphorus, and the available inorganic phosphorus available to plants only accounts for 2% to 3% of the total soil phosphorus. To ensure agricultural production, a large amount of phosphorus fertilizer needs to be invested. However, studies have shown that the s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F11/08C05G3/00C05G3/80C12R1/01
CPCC05F11/08C05G3/00C05G3/80
Inventor 郭金玲孙语健方成龚大春吕育财任立伟杨潇
Owner CHINA THREE GORGES UNIV
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