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Rapid and efficient tissue culture propagation method of ebony

A technology of tissue culture and ebony, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems that the industrialized production system has not yet been established, and achieve the effects of controllable quantity, increased utilization rate, and increased regeneration frequency

Inactive Publication Date: 2021-12-21
LUDONG UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are few domestic literature reports on the rapid propagation of ebony using plant tissue culture technology, and the industrialized production system has not yet been established.

Method used

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  • Rapid and efficient tissue culture propagation method of ebony
  • Rapid and efficient tissue culture propagation method of ebony

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Experimental program
Comparison scheme
Effect test

Embodiment

[0026] A method for rapid propagation of ebony efficient tissue culture, comprising the following steps:

[0027] (1) selection of ebony explants

[0028] Select ebony leaves and foil as tissue culture explants respectively;

[0029] (2) Aseptic treatment of ebony explants

[0030] Disinfect the explants with 75% alcohol for 30s-50s in the ultra-clean workbench, then rinse with sterile water for 2-3 times, then disinfect with 0.1% mercury liter for 5-7min, then rinse with sterile water for 3 times;

[0031] (3) Induction of callus and buds

[0032] Transfer the sterilized explants to a sterile inoculation tray, absorb the excess water on the surface of the explants with sterile filter paper, cut the leaves into 0.5cm×0.5cm in size, cut the foil into 1cm in length, and inoculate into the culture medium cultivated on

[0033] The induction medium of leaf and foil callus and buds are all improved B5 medium + 6-BA 3.0mg / L + NAA0.2mg / L + sucrose 30g / L + agar 8g / L, the formula o...

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Abstract

The invention discloses a rapid and efficient tissue culture propagation method for ebony. The rapid and efficient tissue culture propagation method comprises the steps of selection of ebony explants, sterile treatment of the ebony explants, induction of calluses and buds, propagation of the buds, rooting culture, domestication and transplantation and the like. According to the rapid and efficient tissue culture propagation method for the ebony, ebony leaves and flower stalks are successfully induced and differentiated to form regenerated plants, so that the utilization rate of each part is greatly increased, the regeneration frequency of the explants is remarkably improved, a solid foundation is laid for rapid tissue culture propagation of the ebony, and technical support is provided for ebony seedling industrial production, new variety creation, quality genetic improvement and the like.

Description

technical field [0001] The invention relates to the technical field of tissue culture rapid and efficient propagation, in particular to a tissue culture rapid and efficient propagation method of ebony. Background technique [0002] Echeveria agavoides'Ebony', also known as black sandalwood juice, is a perennial plant of the genus Echeveria (Echeveria) of the sedum family (Crassulaceae), native to Mexico, and has three characteristics: jade bottom , black edge, grain pattern. Ebony is a medium-to-large horticultural species. The leaves of the plant are smooth, relatively wide, and the leaf margins are reddish purple. Under strong light, the color looks dark purple, and the neck is thick. It gradually grows thicker as it grows. Environment, resistant to semi-shade, afraid of waterlogging, avoid sultry heat and humidity, the whole plant grows slowly or completely stops when the temperature is high in summer, it needs to be well ventilated and properly shaded, avoid exposure to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 周厚君杨磊张洪霞
Owner LUDONG UNIVERSITY