Wireless miniature fluorescent microscopic imaging device and application thereof

Abstract

The invention relates to the technical field of medical imaging, and relates to a miniature wireless fluorescence microscope and an imaging method. The total weight is 2.7 g, the total power consumption is about 0.7 W, a low-power-consumption wireless transmitting function and a remote control function are achieved under the condition of high integration level, and the characteristic that multiple devices work at the same time and do not interfere with one another is achieved. According to the imaging device, an acquired fluorescent microscopic image is transmitted to the receiving end through a 5.8 GHz radio-frequency signal, and is stored and played in real time by using a hard disk video recorder. The wireless control function can remotely control the gain value of the microscope, the brightness of an exciting light LED and the on-off of the microscope in the experiment process, so that image parameters and a standby function are regulated and controlled according to needs, and the microscope is turned off to enter a standby state in a time period without imaging so as to reduce power consumption.

Description

technical field [0001] The invention relates to a wireless miniature fluorescent microscopic imaging device and an application thereof, belonging to the field of biological monitoring equipment. Background technique [0002] Fluorescence microscope (Fluorescence microscope) is to irradiate the object under inspection with a light source of specific wavelength to make it emit fluorescence, and then observe the shape and location of the object under the microscope. In the biological field, the imaging of brain cells and blood vessels in freely moving animals is an important means of scientific research in related fields. Fluorescence microscopy can be used to study the absorption and transportation of intracellular substances, the distribution and localization of chemical substances, etc., which provides great help for exploring the structure and function of neural circuits. However, the traditional fluorescence microscope has a large total weight, takes up a lot of space, an...

AI Technical Summary

Problems solved by technology

Although some miniaturized fluorescence microscopes have been reported, most are wired solutions

The solution of wired connection adopts the method of connecting the microscope and the host computer with wires, which leads to a relatively large restriction on the free movement of animals, and some complex behaviors (such as the behavior of mice drilling holes) will be hindered, making it difficult to achieve complete freedom of movement. Deviations between experimental results and natural conditions

In addition, because wires are prone to entanglement, it is also difficult to do behavioral experiments on multiple mice (such as social behavior of multiple mice); the wireless connection solution is to store behavioral data on the back of the animal. implemented in the memory card

This solution does not allow researchers to observe the experimental data in real time during the experiment, but can only take out the SD card to read the data after the experiment is completed, resulting in the inability to effectively monitor the experimental results in real time

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