Method for extracting rebaudioside A, stevioside and stevia polyphenol from stevia rebaudiana and application thereof

A technology of stevia rebaudiana and stevioside, which is applied in the field of extracting active ingredients of plants, can solve the problems of multiple solid wastes, and achieve the effects of strong operability, reduced production costs, and convenient follow-up purification

Pending Publication Date: 2021-12-31
HUNAN NUSTREETCARAX
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When extracting steviol glycosides, iron salts and calcium salts are usually used to complex and remove impurities, which not only incorporates stevia polyphenols into the waste residue, but worse, it also produces more organic and inorganic mixed solid waste

Method used

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  • Method for extracting rebaudioside A, stevioside and stevia polyphenol from stevia rebaudiana and application thereof
  • Method for extracting rebaudioside A, stevioside and stevia polyphenol from stevia rebaudiana and application thereof
  • Method for extracting rebaudioside A, stevioside and stevia polyphenol from stevia rebaudiana and application thereof

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Embodiment 1

[0057] The embodiment of the present invention provides a method for extracting rebaudioside A, stevioside and stevia polyphenols from Stevia rebaudiana, which specifically includes the following steps:

[0058] S1, water extraction

[0059] Weigh 100g of stevia leaf raw material after drying and pulverizing, use 1000ml of pure water to reflux and extract at a temperature of 80°C for 90min, separate the filter residue and filtrate, and then use 700ml of pure water to reflux and extract for 60min under the same conditions. time, combined 3 times of water extracts obtained, concentrated to 300ml;

[0060] S2, precipitation

[0061] Add saturated lime water to the concentrated solution obtained in S1 to adjust its pH = 10, and centrifuge after standing for 1 hour to obtain supernatant and precipitate;

[0062] S3. Purification of steviol glycosides

[0063] Take the supernatant obtained in S2 and put it on the D101 macroporous resin column, and control the sample loading flow ...

Embodiment 2

[0067] The embodiment of the present invention provides a method for extracting rebaudioside A, stevioside and stevia polyphenols from Stevia rebaudiana, which specifically includes the following steps:

[0068] S1, water extraction

[0069] Weigh 1000g of the stevia leaf raw material after drying and pulverizing, use 12000ml of pure water to reflux and extract at a temperature of 78°C for 100min, separate the filter residue and filtrate, and then use 9000ml of pure water to reflux and extract for 90min under the same conditions. time, combined 3 times of obtained water extracts, concentrated to 3500ml;

[0070] S2, precipitation

[0071] Add saturated lime water to the concentrated solution obtained in S1 to adjust its pH=9.5, let it stand for 80 minutes, and centrifuge to obtain supernatant and precipitate;

[0072] S3. Purification of steviol glycosides

[0073] Take the supernatant obtained in S2 and put it on the AB-8 macroporous resin column, and control the sample lo...

Embodiment 3

[0077] The embodiment of the present invention provides a method for extracting rebaudioside A, stevioside and stevia polyphenols from Stevia rebaudiana, which specifically includes the following steps:

[0078] S1, water extraction

[0079] Weigh 100kg of dried and pulverized stevia leaf raw materials, use 1000L of pure water to reflux and extract at a temperature of 80°C for 90 minutes, separate the filter residue and filtrate, and then use 800L of pure water to reflux and extract for 60 minutes under the same conditions. time, combined 3 times of water extracts obtained, concentrated to 250L;

[0080] S2, precipitation

[0081] Add saturated lime water to the concentrated solution obtained in S1 to adjust its pH = 10.0, let stand for 1 hour and then centrifuge to obtain supernatant and precipitate;

[0082] S3. Purification of steviol glycosides

[0083] Take the supernatant obtained in S2 and put it on the HP-20 macroporous resin column, and control the sample loading f...

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Abstract

The invention discloses a method for extracting rebaudioside A, stevioside and stevioside polyphenol from stevia rebaudiana and application thereof. The method comprises the following steps: adding saturated lime water into a leaching solution obtained by leaching stevia rebaudiana powder with hot water to adjust the pH value, standing and precipitating, and performing centrifugal separation; performing post-treatment on the centrifuged supernate to obtain a stevioside crude product, then adding n-propyl alcohol, heating, filtering while hot, and respectively purifying a filtrate and a filter cake to obtain stevioside and rebaudioside A; and adding diluted hydrochloric acid into the centrifuged precipitate to dissolve, adjusting the pH value, centrifuging, taking the supernatant, and purifying with polyamide resin to obtain the stevia polyphenol. According to the method, two main components in stevioside can be efficiently separated by utilizing the characteristic that rebaudioside A and stevioside have different solubilities in n-propanol; and the purification time can be shortened by crystallizing the rebaudioside A crude product with the purity of 80% or above, the situation that rebaudioside A is partially dissolved in mother liquor and cannot be fully separated out during direct crystallization is avoided, and stevioside with the content of 95% or above can be obtained.

Description

technical field [0001] The invention relates to the technical field of extraction of effective plant components, and more specifically relates to a method for extracting rebaudioside A, stevioside and stevia polyphenols from stevia rebaudiana and its application. Background technique [0002] Low-calorie sweeteners are increasingly sought after by consumers amid serious health concerns associated with increased consumption of sucrose. [0003] Steviol glycosides are a group of sweet substances rich in stems and leaves of Stevia rebaudiana (Stevia reabudiana Bertoni), and its main components are rebaudioside A (RA) and stevioside (STV), accounting for about 90 wt% of steviosides. Steviol glycosides are safe, non-toxic and stable in physical and chemical properties. It is a kind of sweetener with high sweetness and low calorie value. It can reduce the incidence of diabetes and obesity while meeting the demand for sweetness, protect the kidneys and promote oral health. role. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/256C07H1/08A61K36/287
CPCC07H15/256C07H1/08A61K36/287A61K2236/331A61K2236/39A61K2236/53A61K2236/51A61K2236/55
Inventor 范斌张宝堂谭家忠廖娜雷桂芳
Owner HUNAN NUSTREETCARAX
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