Heavy metal adsorption microbial inoculum and application thereof in soil pollution remediation
A technology for adsorbing heavy metals and heavy metals, applied in the field of biological treatment of environmental pollutants, can solve the problems of low cost of microbial remediation technology, and achieve the effects of high efficiency stabilization, improvement of soil basic environment, and efficiency improvement
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Embodiment 1
[0033] Isolation and identification of embodiment 1 bacterial strain:
[0034] In the present invention, the HHS1 strain of Spirulina spirulina is obtained by screening and acclimating in the soil of livestock and poultry breeding grounds polluted by heavy metal-antibiotics, and the specific steps are as follows:
[0035] A. Use oxytetracycline to screen antibiotic-resistant bacteria in polluted soil from livestock and poultry breeding:
[0036] The domestication process is: collect contaminated soil samples piled up by animal manure produced by livestock and poultry farms, and after knowing the initial oxytetracycline concentration in the soil samples, add the samples to the inorganic salt medium containing oxytetracycline Carry out gradient domestication, the concentration of oxytetracycline in the culture medium increases gradually, successive concentration is respectively 50 μ g / kg, 100 μ g / kg, 200 μ g / kg, 500 μ g / kg, 1 mg / kg, 2 mg / kg), each concentration is in Incubate i...
Embodiment 2
[0054] The obtained HHS1 strain is transferred to a new liquid inorganic salt medium, and 10 mg / L Cu is added to the inorganic salt medium 2+ solution and 10mg / L of Zn 2+ The solution was used as the initial concentration for acclimatization and cultured in a shaker at 15°C and 150r / min in the dark for 7 days, and then gradually increased Cu in the inorganic salt medium. 2+ and Zn 2+ The concentration of Cu 2+ and Zn 2+ Concentrations are 10mg / L, 20mg / L, 30mg / L, 40mg / L, 50mg / L in turn, and each time the concentration is increased, culture in a shaker at 15°C and 150r / min in the dark for 7 days, and observe the bacteria in the medium regularly liquid state, the liquid in the medium changes from transparent to turbid, indicating that the bacterial solution grows well; the domesticated HHS1 is transferred every three days, and repeated six times. 2+ and Zn 2+ The concentration was 50mg / L, and cultured in a shaker at 15°C and 150r / min in the dark for 3 days; 2+ and Zn 2+ HH...
Embodiment 3
[0056] Soil remediation test of copper in soil stabilized by immobilized microbial agent at low temperature (15°C): Preparation of agent:
[0057] 1. The HHS1 heavy metal tolerant bacterial strain domesticated in the above-mentioned embodiment 2 is activated in a conventional manner with a liquid medium, and after activation, the bacterial suspension is inoculated into the LB medium, and its content accounts for 2wt% of the LB medium and is added at 15 Cultivate at constant temperature for 48 hours to the logarithmic phase, collect the culture solution and resuspend to obtain the bacterial solution. Specifically, the HHS1 strain was inoculated in LB medium, and after culturing to the logarithmic phase, the bacteria were washed down with sterile saline to configure OD 600 1.0 bacterial suspension.
[0058] 2. Dry and grind the discarded mushroom sticks after planting mushrooms, put them in a muffle furnace, and burn them at 500°C for 4 hours to form biochar from fungus chaff; ...
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Abstract
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