Method for establishing pulmonary fibrosis rhesus monkey model

A pulmonary fibrosis and animal model technology, applied in the field of animal models, can solve the problems of injury, many times of administration, and complicated modeling methods, and achieve the effects of obvious pulmonary fibrosis, simple production and broad application prospects.

Pending Publication Date: 2022-01-04
WEST CHINA HOSPITAL SICHUAN UNIV +1
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Problems solved by technology

This formula uses paraquat and bleomycin to model at the same time. The number of administrations is more, the modeling method is complicated, and the administration of the trachea will cause local damage, which is inconsistent with the clinical manifestations of the patient, so it cannot be used for pulmonary fibrosis. Efficacy and safety evaluation of therapeutic drugs

Method used

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  • Method for establishing pulmonary fibrosis rhesus monkey model
  • Method for establishing pulmonary fibrosis rhesus monkey model
  • Method for establishing pulmonary fibrosis rhesus monkey model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The preparation method of embodiment 1 secondary pulmonary fibrosis animal model

[0030] 1. Materials and methods:

[0031] 1.1 Experimental materials

[0032] Three 3-4-year-old female and male rhesus monkeys, free from herpes B virus, simian retrovirus, simian leukemia virus and immunodeficiency virus infection; purchased from Chengdu Pingan Animal Breeding Base.

[0033] 1.2 Main reagents

[0034] Paraquat (abbreviated as PQ) was purchased from Shanghai Pesticide Research Institute;

[0035] Other medicines and consumables were purchased from Likang Pharmacy of West China Hospital.

[0036] 1.3 Preparation of the main solution

[0037] Solution preparation:

[0038] Take the original 1g / bottle of paraquat powder, add 1mL of physiological saline to dissolve it, and use it as a storage solution for short-term storage at 4°C. According to the weight of the monkeys, the PQ solution was incorporated into the food of the monkeys at 25 mg / kg body weight.

[0039] 1....

experiment example 1

[0085] Experimental Example 1 Constructing the dose screening experiment of animal model of secondary pulmonary fibrosis

[0086] 1. According to the model building method in Example 1, the only difference is that the dosage in "1.5.4 Paraquat Feeding Rhesus Monkey Process" was increased from 25 mg / kg to 40-80 mg / kg. It was found that the rhesus monkeys eventually developed symptoms of acute lung injury, which did not meet the modeling requirements.

[0087] That is to say, using the modeling method of Example 1, the rhesus monkey model of secondary pulmonary fibrosis cannot be successfully established when the oral administration dose is increased to 40-80 mg / kg.

[0088] 2. According to the model building method in Example 1, the only difference is that the dosage in "1.5.4 Paraquat Feeding Rhesus Monkey Process" was reduced from 25 mg / kg to 10 mg / kg, and it was found that the rhesus monkey lung The presence of an inflammatory infiltrate rather than secondary pulmonary fibr...

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Abstract

The invention discloses a method for establishing a pulmonary fibrosis rhesus monkey model, and belongs to the field of animal models. The method comprises the following steps that paraquat is applied to animals, and the application dosage is 20-35 mg/kg; an animal is a non-human primate animal. According to the method for establishing the pulmonary fibrosis rhesus monkey model, a secondary pulmonary fibrosis animal model constructed by the method has the advantages of simplicity in manufacturing, no local injury caused by single administration and administration mode, obvious pulmonary fibrosis and the like; and various biochemical indexes, disease process and progression of the animal model are highly similar to those of human secondary pulmonary fibrosis; the animal model provides a long enough treatment window period, and can be used for evaluating the curative effect and safety of various secondary pulmonary fibrosis treatment drugs which cannot be completed by rodent animal models.

Description

technical field [0001] The invention belongs to the field of animal models, and in particular relates to a method for establishing a rhesus monkey model of pulmonary fibrosis. Background technique [0002] Pulmonary fibrosis generally refers to the pulmonary inflammatory response caused by different factors such as poison, spontaneous immune response, drug side effect, infection, severe trauma, etc., with alveolar surface epithelial cell damage, inflammatory cell accumulation, fibroblast proliferation, extracellular A group of diseases characterized by matrix deposition and scar formation, which eventually lead to structural changes and loss of function of normal lung tissue. Pulmonary fibrosis is usually divided into two types. One is idiopathic pulmonary fibrosis (IPF). IPF is fibrosis with unknown etiology. The other is secondary pulmonary fibrosis (SPF). The common causes of SPF are Sjogren's syndrome, polymyositis, rheumatoid arthritis and systemic sclerosis. Due to t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/027A61K49/00
CPCA01K67/027A61K49/0008A01K2207/20A01K2227/106A01K2267/03
Inventor 姚蓉石毓君武振汝邵明洋罗徐王洪润
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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