Extraction method of coenzyme Q10

Abstract

The invention provides an extraction method of coenzyme Q10, which comprises the following steps: extracting mycelium of coenzyme Q10 to obtain an extract liquid, and adding sodium hydroxide during extraction; concentrating the extract liquid, adding an aqueous solution of caustic soda flakes after concentration, stirring, and discharging; and centrifuging the obtained material for multiple times to obtain a coenzyme Q10 crude product. According to the method, the sodium hydroxide is added in the extraction process of the coenzyme Q10, so that the sodium hydroxide and lipids in mycelia are hydrolyzed to generate carboxylate and alcohol, and subsequent purification of the coenzyme Q10 is easier; the aqueous solution of caustic soda flakes is added after the extract liquid is concentrated, and can hydrolyze lipids in the concentrated solution and react with a small amount of water in the concentrated solution to generate heat to accelerate the concentration rate due to the characteristic that the aqueous solution of the caustic soda flakes is high polymer electrolyte; the polar solvent acetone in the extracting agent can be recycled for multiple times and then used mechanically, so that the production cost is saved, and the process can be suitable for large-scale production.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a method for extracting coenzyme Q10. Background technique [0002] Coenzyme Q10 is a fat-soluble antioxidant, a fat-soluble quinone compound that exists in nature, and its structure is similar to vitamin K, vitamin E and plastoquinone. Participate in energy production and activation in human body cells. It is the most effective antioxidant ingredient to prevent arteriosclerosis. It can activate the nutrition of human cells and cell energy. It can improve human immunity, enhance anti-oxidation, delay aging and enhance human vitality. Function, it is widely used in cardiovascular system diseases in medicine, and it is widely used in nutritional health products and food additives at home and abroad. [0003] Initially, Japan realized the synthesis and production of coenzyme Q10 by extracting solanesol from tobacco leaves as a raw material, which greatly reduced the cost of coenzyme Q1...

AI Technical Summary

Problems solved by technology

In addition, the technology of semi-chemical synthesis of coenzyme Q10 is relatively mature, and industrialization has been realized. The product cost is low and the price is moderate. products have a large gap

The biological extraction method produces natural and organic products, which are easily absorbed and transformed by the human body, while the chemical synthesis method produces artificially chemically synthesized organic products, which have extremely poor biological activity, are not easily absorbed by the human body, and are difficult to fully exert the pharmacology of coenzyme Q10 effect

In recent years, some countries have realized the production of coenzyme Q10 by microbial fermentation. This new bioengineering method not only combines the advantages of the biological extraction process and the chemical synthesis process, but also overcomes their shortcomings. However, in the biological extraction process there are still problems

[0004] Since coenzyme Q10 is a fat-soluble substance with poor water solubility, most of the current extraction processes are based on non-polar solvent extraction, such as petroleum ether and n-hexane; while extracting coenzyme Q10, other fat-soluble impurities will also be included. Extracted, including bacterial pigments, non-polar lipids, neutral lipids, polar lipids and quinone homologues; at the same time, the content of these fat-soluble impurities extracted together with coenzyme Q10 will vary with the extraction solvent. Sometimes the content of impurities is much greater than that of the extracted coenzyme Q10, which brings difficulties to the subsequent dissolution and purification process

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