Chlamydomonas reinhardtii strains capable of efficiently adsorbing copper ions

Abstract

The invention provides chlamydomonas reinhardtii strains capable of efficiently adsorbing copper ions. The three strains are respectively named Chlamydomonasreinhardtii Cu81, Cu87 and Cu92, and the preservation numbers are respectively CGMCC No. 21484, CGMCC No. 21485 and CGMCC No. 21486. According to the invention, a chlamydomonas mutant library (resistance gene fragment aphVIII is randomly integrated into a chlamydomonas genome to cause random insertion mutation) is constructed, and a corresponding screening method is established to screen potential chlamydomonas reinhardtii strains capable of adsorbing metal copper, so that in subsequent research, mutant genes can be conveniently searched through the known sequence of the aphVIII fragment, the potential molecular mechanism of the strains in the aspects of heavy metal metabolism, resistance gene function and the like can be deeply researched, and a solid foundation is laid for further excavating the potential of the heavy metal adsorption strains in genetic engineering improvement.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a Chlamydomonas algae strain that efficiently absorbs copper ions. Background technique [0002] The rapid development of the economy is accompanied by the intensification of environmental pollution. Environmental problems have seriously affected the ecological balance and threatened human health. One of the environmental problems is the massive generation of heavy metal sewage, which is related to many industrial productions, such as printing and dyeing, steel, smelting, electroplating, paint, coating and other industries. The deterioration of environmental quality caused by heavy metals has aroused people's strong concern. Therefore, it is urgent to increase the research on the prevention and control technology of heavy metals. [0003] Copper is a heavy metal, and it is also an essential trace element for animals, plants and humans. Trace amounts of copper can promote ...

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Problems solved by technology

[0006] According to background technology research, most of the current research on the removal of heavy metals from algae is mostly based on the study of different algae (Chlamydomonas, Volvox, Spirulina, Nostoc, Anabaena, Red Algae, etc.), different adsorption conditions (mainly including adsorption time , the pH value of the adsorption solution, the concentration of adsorbed heavy metals, the complexity and proportion of heavy metal types, etc.) and the modification and combination of different biological materials (modification and change of functional groups, joint action with plant cells, fungal cells or bacterial cells), and for its main The determinants of genes have received less attention

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