Trichoderma atroviride and application thereof in inhibiting pathogenic bacteria of ginseng

A technology of Trichoderma viridans and ginseng, which is applied in the field of ginseng planting in farmland, can solve the existing problems, achieve the effects of protecting the population structure, improving the cultivation environment, and improving the yield and quality

Active Publication Date: 2022-01-11
INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, there is no Trichoderma viri

Method used

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  • Trichoderma atroviride and application thereof in inhibiting pathogenic bacteria of ginseng
  • Trichoderma atroviride and application thereof in inhibiting pathogenic bacteria of ginseng
  • Trichoderma atroviride and application thereof in inhibiting pathogenic bacteria of ginseng

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] This example provides screening, isolation and identification methods of Trichoderma atroviride Tri802 strain.

[0059] The trichoderma dark green provided by the invention is isolated from the ginseng rhizosphere soil of the ginseng planting base in Zuojia Town, Jilin City, Jilin Province.

[0060] The rhizosphere soil of ginseng was collected from the ginseng planting base in Zuojia Town, Jilin City, Jilin Province, and the rhizosphere soil was obtained by the dilution coating plate method. Take 10g of ginseng rhizosphere soil and add it to a conical flask filled with 90mL of sterile water, shake it on a shaker at 150r / min for 30min, draw 1mL of uniformly mixed soil suspension from the conical flask, and perform gradient dilution with sterile water. from 10 -3 、10 -4 、10 -5 Take 200 μL of the three gradients and add it to the PDA medium plate to spread evenly. Place the plate at 25°C for 3 days, pick the mycelium of a single Trichoderma colony on the plate and tran...

Embodiment 2

[0066] In this example, the antagonism experiment of Trichoderma dark viride on pathogenic bacteria of ginseng was carried out.

[0067] Pathogens of 5 major diseases of ginseng were selected: Alternaria panax, Sclerotinia schinseng, Colletotrichum panacicola, Ilyonectria robusta, and root rot of ginseng (Fusarium oxysporum), the above five strains were provided by the medicinal plant cultivation team of the Institute of Special Products, Chinese Academy of Agricultural Sciences. The preserved strain Tri802 and the pathogenic strain were transferred to PDA plates respectively, and after constant temperature cultivation at 25°C in the dark for 7 days, the bacterial cake was punched out from the edge of the colony with a 5mm hole puncher. The two-point confrontation plate culture method was adopted, and they were placed on the symmetrical sides of the PDA plate respectively, with a distance of 3 cm between the bacteria cakes. Only pathogenic bacteria were inoculated as the contr...

Embodiment 3

[0071] In this example, the growth-promoting effect experiment of Trichoderma dark viride on ginseng cultivated in farmland soil was carried out.

[0072] In this example, a pot test method was used to measure the growth-promoting effect of Trichoderma dark green Tri802 strain on ginseng.

[0073] The preparation method of bacterial strain culture medium is as follows:

[0074] Activate the slanted surface of Tri802 test tube of Trichoderma dark viride, take two 5mm bacteria cakes and inoculate them in 100mL potato dextrose liquid medium (PDB) packed in a 250mL triangular bottle, and cultivate them at 170r / min, 25°C for 48h to obtain the seed liquid; The Tri802 seed liquid of Trichoderma dark viridifera was inoculated in the fermentation medium with 10% (volume ratio) and cultivated at 170r / min, cultivated for 96h at 25°C to obtain the culture medium; Filtrate with bacterial gauze, count the filtrate with a hemocytometer, and disperse the spore suspension to 6 g·L -1 In sodium...

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Abstract

The invention discloses trichoderma atroviride and application thereof in inhibiting pathogenic bacteria of ginseng, and relates to the technical field of ginseng cultivation in farmland. Trichoderma atroviride is separated and screened from soil, and a ginseng pathogenic bacterium inhibition test shows that the screened trichoderma atroviride can effectively inhibit the growth of various ginseng pathogenic bacteria. That is to say, the trichoderma atroviride provided by the invention has a certain prevention and treatment effect on diseases and has a good biocontrol application prospect. In addition, the trichoderma atroviride has a remarkable growth promoting effect on growth of roots, stems and leaves of ginseng. After the trichoderma atroviride is applied, the existing ginseng cultivation environment can be improved to a great extent, so that the yield and quality of the ginseng can be improved. The trichoderma atroviride can protect the population structure of soil microorganisms.

Description

technical field [0001] The invention relates to the technical field of planting ginseng in farmland, in particular to a kind of Trichoderma dark viride and its application in inhibiting ginseng pathogenic bacteria. Background technique [0002] Trichoderma (Trichoderma spp.) is a kind of soil-habitual filamentous fungus distributed all over the world, which has good biocontrol potential for various plant diseases. Its biocontrol mechanism mainly includes reparasitic and antibiotic effects on pathogenic bacteria, competition, growth promotion and resistance induction on plants. At present, there is no Trichoderma viridans against ginseng pathogenic bacteria. [0003] In view of this, the present invention is proposed. Contents of the invention [0004] The object of the present invention is to provide a kind of Trichoderma dark viride and its application in inhibiting ginseng pathogenic bacteria to solve the above technical problems. [0005] The present invention is ach...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/38A01P3/00A01P21/00C09K17/14C12R1/885C09K101/00
CPCC12N1/14A01N63/38C09K17/14C09K2101/00
Inventor 王秋霞马莹莹金桥张悦张淋淋张舒娜
Owner INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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