Mycoplasma drug sensitivity fluorescence detection system and detection method

Abstract

The invention discloses a mycoplasma drug sensitivity fluorescence detection system. The mycoplasma drug sensitivity fluorescence detection system comprises a control module, the control module is electrically connected with a driving module, the driving module is electrically connected with a refrigeration sheet and a heating resistor, the control module is electrically connected with a processing module, the processing module is electrically connected with a temperature sensor and a fluorescence detection module, and the control module is electrically connected with a timing module, a light emitting module, a lighting module and an auxiliary module. According to the mycoplasma drug sensitivity fluorescence detection system, the drug sensitivity of mycoplasma is detected through a PCR fluorescence detector, the detection operation is convenient and rapid, the device is small and exquisite and convenient to carry, a sealing plate and a carrying disc are elastically supported, liquid medicine on the carrying disc is prevented from being scattered due to collision, and stable sealing connection can be kept; and the internal temperature of the equipment is adjusted through the refrigeration sheet and the heating resistor, and the processing module is arranged, so that detected data information is effectively processed.

Description

technical field [0001] The invention belongs to the technical field of medical detection, and in particular relates to a mycoplasma drug-sensitivity fluorescence detection system and detection method. Background technique [0002] Mycoplasma is a kind of the smallest prokaryotic cell-type microorganisms without cell wall, highly pleomorphic, able to pass through bacteria filters, and can be cultivated and proliferated in artificial medium, with a size of 0.1-0.3 microns. Because it can form filamentous and branched shapes, it is called mycoplasma. Mycoplasma widely exists in humans and animals, and most of them are not pathogenic. Mycoplasma pathogenic to humans mainly include Mycoplasma pneumoniae, Ureaplasma urealyticum, Mycoplasma hominis, and Mycoplasma genitalium. Macrophages, IgG and IgM all have a certain killing effect on mycoplasma. Gram staining is negative, but it is not easy to stain. Generally, Giemsa staining is used to stain lavender. Mycoplasma mainly repro...

AI Technical Summary

Problems solved by technology

Mycoplasma resistance is weak, sensitive to heat and drying, sensitive to 75% ethanol, creol soap solution, sensitive to erythromycin, tetracycline, spiramycin, streptomycin, kanamycin and other drugs, but sensitive to penicillins antibiotics are not sensitive, but there are still various problems in various mycoplasma drug susceptibility tests on the market

[0003] For example, the Mycoplasma pneumoniae drug resistance diagnostic kit disclosed by the authorized announcement number CN103820557B, although it has realized that the provided Mycoplasma pneumoniae drug resistance diagnostic kit can identify the genotype of the MP2063 locus by establishing a fluorescent quantitative PCR allele identification method. , to judge its drug resistance while rapidly diagnosing, and the detection sensitivity is good, but it does not solve the problems that the existing mycoplasma detection needs manual judgment, and cannot effectively realize automatic operation. Therefore, we propose a mycoplasma drug sensitivity Fluorescence detection system and detection method

Images