Stem-cell perfusion nutrient solution for in-vivo tissue and organ regeneration and application method of stem-cell perfusion nutrient solution

A tissue organ and stem cell technology, applied in the direction of bone/connective tissue cells, tissue culture, cell culture active agent, etc., can solve problems such as imperfection and slow bone formation

Pending Publication Date: 2022-01-14
THE SECOND AFFILIATED HOSPITAL TO NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • Stem-cell perfusion nutrient solution for in-vivo tissue and organ regeneration and application method of stem-cell perfusion nutrient solution
  • Stem-cell perfusion nutrient solution for in-vivo tissue and organ regeneration and application method of stem-cell perfusion nutrient solution
  • Stem-cell perfusion nutrient solution for in-vivo tissue and organ regeneration and application method of stem-cell perfusion nutrient solution

Examples

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Effect test

Embodiment 1

[0029] A stem cell perfusion nutrient solution for the regeneration of tissues and organs in the body, the raw materials include: 1×10 bone marrow stromal stem cells 6 ~1×10 7 1 / ml, recombinant human basic fibroblast growth factor 2ng / ml, human recombinant bone morphogenetic protein 25ng / ml, dexamethasone 0.25~1.0mg / ml, vitamin D3 1~2mg / ml.

[0030] Bone marrow stromal stem cells are obtained in the following ways:

[0031] S1: Use a syringe containing heparin anticoagulant to obtain 200ml of bone marrow blood, and put the obtained bone marrow blood into a medical sterile blood collection bag for backup;

[0032] S2: Mix the bone marrow blood with an equal volume of PBS, and let it stand at room temperature for 30 minutes, absorb the Percol l solution with a density of 1.073g / ml, and make the ratio of the separated liquid to 1:1; use a horizontal centrifuge at 2000r / min Centrifuge for 20-40 minutes, so that the liquid in the centrifuge tube is divided into three layers from ...

Embodiment 2

[0037] A method for applying stem cell perfusion nutrient solution for tissue and organ regeneration in vivo, comprising the steps of:

[0038] Step (1) Use a syringe to draw stem cell perfusion nutrient solution (which contains bone marrow stromal stem cells) used for regeneration of tissues and organs in the body and pour it into the Porite coral scaffold, and ensure complete infiltration and no leakage to form bone marrow stromal stem cells (BMSC)-Porite Coral scaffold (CN) complex;

[0039] Step (2) will place the bone marrow stromal stem cells-Porites scaffold complex (BMSC-CN) at 37 degrees Celsius with a volume fraction of 5% CO 2 After 5 hours in the incubator, DMEM complete medium was added for culture, and the bone marrow stromal stem cells-Porite coral scaffold complex was cultured for 5 days. After the bone marrow stromal stem cells and Porite coral scaffolds were firmly attached, they were implanted back into the bone defect.

[0040] In this embodiment, the stru...

Embodiment 3

[0050] The difference between embodiment three and embodiment one is that, as attached Figure 4 As shown, in the present embodiment, the coral support 11 made of Porites is improved, and some contact grooves 13 are provided on the side wall of the coral support 11, and the contact grooves 13 and the coral support 11 are in a smooth transition, and the through holes 12 of the coral support It is tapered, that is, the upper diameter of the through hole is larger than the lower diameter.

[0051] The specific implementation process is as follows:

[0052] In the present embodiment, the contact groove 13 arranged on the periphery of the coral support 11, the contact groove 13 increases the surface area of ​​the coral support 11, and then provides a larger surface area for the attachment and proliferation of bone cells, thereby making the proliferation of bone cells more effective. Big.

[0053] As in Example 2, the subcutaneous implantable drug delivery device consists of two p...

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Abstract

The invention relates to the technical field of tissue and organ regeneration and discloses a stem-cell perfusion nutrient solution for in-vivo tissue and organ regeneration and an application method of the stem-cell perfusion nutrient solution. The stem-cell perfusion nutrient solution is prepared from bone-marrow stromal stem cells, recombinant human basic fibroblast growth factors, human recombinant bone morphogenetic proteins, dexamethasone and vitamin D3. According to the application method of the stem-cell perfusion nutrient solution, the stem-cell perfusion nutrient solution is perfused onto a Porites stent to form a compound, and the compound has the effect of repairing parenchymal organs.

Description

technical field [0001] The invention relates to the technical field of tissue and organ regeneration, in particular to a stem cell perfusion nutrient solution for tissue and organ regeneration in vivo, an application method thereof and an extraction device thereof. Background technique [0002] The regenerative repair of large segmental load-bearing bone defects is extremely difficult, and the use of autologous bone grafts will cause bleeding, infection, and new injuries, and the source is limited. Large bone defects caused by trauma, infection, tumors, burns, etc. are difficult problems in clinical treatment. Traditional autologous bone or allogeneic bone grafts and biomaterials are used to fill them. Due to various drawbacks, the therapeutic effect is not ideal. limit its clinical application. [0003] In recent years, bone marrow stromal stem cells (BMSC)-based tissue engineering bone transplantation has become a research hotspot in the treatment of large bone defects. ...

Claims

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Application Information

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IPC IPC(8): C12N5/077C12N5/0775
CPCC12N5/0663C12N5/0655C12N2501/115C12N2501/155C12N2500/38C12N2501/30
Inventor 吴嘉强戴江华罗威陈依琳周依婷
Owner THE SECOND AFFILIATED HOSPITAL TO NANCHANG UNIV
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