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Method for preparing nano-silver based on red spinach extracting solution

The technology of red amaranth and nano-silver is applied in the fields of biotechnology and antibacterial applications, which can solve the problems of high preparation technology requirements, pollute the environment, etc., and achieve the effects of strong operability, less pollution and mild reaction conditions.

Active Publication Date: 2022-02-01
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, chemical methods are more common, but most of the reducing agents and stabilizers used are toxic reagents, which seriously pollute the environment. At the same time, the reaction conditions need to be strictly controlled, and the requirements for the preparation technology are very high.

Method used

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  • Method for preparing nano-silver based on red spinach extracting solution
  • Method for preparing nano-silver based on red spinach extracting solution
  • Method for preparing nano-silver based on red spinach extracting solution

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The leaves of red amaranth were picked, washed three times with ultrapure water, dried at 65°C for 2 days, pulverized by a pulverizer, and passed through a 40-mesh sieve to obtain red amaranth powder. Weigh 5g of red amaranth powder, add 150ml of 70% ethanol aqueous solution, heat reflux at 85°C for 2h, keep stirring during the period, cool to room temperature; 300W, ultrasonic 3sec off and 3sec in an ice bath for 20min, and 200 mesh gauze to filter the extract , and filter the extract with a 0.22 μM filter membrane to obtain a red amaranth extract. AgNO with a concentration of 12mmol / L 3 The solution and the red amaranth extract were mixed according to the volume ratio of 8:1, heated and stirred at 80° C. in the dark for 30 minutes to prepare a nano-silver solution.

[0029] figure 1 It is the ultraviolet-visible light absorption curve measured at 350-650nm for the synthesized nano-silver solution and the silver nitrate extract. Wherein: a is the extract solution of...

Embodiment 2

[0031] Embodiment 2: the antibacterial performance appraisal of the nano silver solution synthesized in embodiment 1

[0032] (1) Strain cultivation: Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa were inoculated in MH liquid medium, and cultivated to logarithmic growth phase at 37°C and 180r / min.

[0033] (2) Inhibition zone test: Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa in the logarithmic growth phase were diluted to 10 8 CFU / mL, 100 μL each was evenly spread on the MH solid medium. A number of sterilized filter paper pieces were respectively immersed in the nano-silver solution synthesized in Example 1, red amaranth extract and physiological saline, and the above-mentioned filter paper pieces were attached to the bacteria containing Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa. On the MH plate, incubate at a constant temperature of 37°C for 16 hours, measure the diameter of the inhibition zone, and take th...

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Abstract

The invention discloses a method for preparing nano-silver based on a red spinach extracting solution. The method is characterized by mixing the red spinach ethanol extracting solution with the silver ion solution, and reacting to generate the nano-silver. According to the method, the red spinach extracting solution is used for reducing silver ions to prepare the simple substance nano-silver. The nano-silver particles with the particle size of 1-30 nm can be prepared according to the method provided by the invention. The nano-silver particles are prepared from the red spinach extracting solution, the preparation method is simple and easy to implement, high in operability and low in cost, the red spinach extracting solution serves as a reducing agent and a stabilizing agent at the same time, other chemical components do not need to be added in the reaction process, a large number of nano-silver particles are prepared within a short time, the method is a simple and environment-friendly nano-silver preparation method, and the prepared nano-silver solution has a high antibacterial effect.

Description

Technical field: [0001] The invention belongs to the field of biotechnology and antibacterial application, in particular to a method for preparing nano-silver based on red amaranth extract. Background technique: [0002] Nano silver (Silver nanoparticles, referred to as Ag-NPs) is called a new generation of antibacterial agent in the 21st century. It has broad-spectrum and high-efficiency antibacterial performance, long-lasting antibacterial, strong permeability, safe and non-toxic, repairing and regenerating, and is not easy to make microorganisms Advantages of developing drug resistance. Nanosilver has been widely used in various medical materials, such as wound dressings, surgeon's masks, medical catheters, medical surgical instruments, implanted in human tissues, etc. Based on the excellent antibacterial performance of nano-silver, its application prospect is broad, and it has a wide range of market demands in the fields of daily necessities, medical and health care, bu...

Claims

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Application Information

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IPC IPC(8): B22F9/24B22F1/054B82Y40/00
CPCB22F9/24B82Y40/00
Inventor 谢小保张志清李文茹姚俊伟刘德赞施庆珊
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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