Stenotrophomonas rhizophila capable of inhibiting generation of nitrosamine and application thereof
A oligotrophomonas and nitrosamine technology, applied in the field of microorganisms, can solve the problems of the original flavor of food, adverse effects on taste, unclear specific components, and difficulty in implementation, and achieves prolonging the fresh-keeping time of cooked food and inhibiting nitrosative Amine formation, inhibition of harmful transformations
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Embodiment 1
[0022] Example 1: Isolation and Identification of Bacterial Strains
[0023] 1) Sampling: Obtain caviar with a storage time of 6 months from Zhejiang Quzhou Sturgeon Technology Co., Ltd.;
[0024] 2) Take 3g of fish eggs and add 6mL of sterile saline to obtain a bacterial solution, and incubate at 37°C for 2h;
[0025] 3) Take 100 μL of the above bacterial solution, add 900 μL sterile saline to dilute the bacterial solution to 10%, and gradually dilute the bacterial solution to 10% according to the above operation. 6 100 μL of the bacterial solution was added to LB nutrient agar medium by the spread plate method, and cultured at 37 °C for 24 h.
[0026] 4) The samples were sent to Shanghai Meiji Biomedical Technology Co., Ltd. for high-throughput sequencing to identify 6 strains.
[0027] One of the strains was identified, the strain genome was extracted, its 16S rDNA sequence was PCR-sequenced, and its species was identified by Blast comparison. The results showed that the ...
Embodiment 2
[0028] Embodiment 2: the application of bacterial strain inhibition nitrosamine generation
[0029] The experimental object is canned luncheon meat with 0.5% sodium nitrite added, and the experiment is carried out by using the freeze-dried powder of the fermented broth of Stenotrophomonas rhizophila SR-1. 100g of canned luncheon meat and sausage were added in each group with 0.5% sodium nitrite, and no other additions were made in the control group; Put it on a flat plate, put it in a sealed fresh-keeping box with canned luncheon meat and sausage respectively, and place it at 28°C for 3 days. Then, according to GB 5009.26-2016 "Determination of N-Nitrosamines in Food Safety National Standard Food", the determination of nitrosamines in canned luncheon meat and sausages was carried out. figure 2 .
[0030] The results showed that after the canned luncheon meat and sausage were stored at 28°C for 3 days, the content of N-dimethylnitrosamine (NDMA) in the experimental group was...
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