Anti-aging cream containing Changbai mountain wild ginseng cell water extract
A technology of water extract and wild ginseng, applied in the field of skin care products, can solve problems that have not yet been reported, and achieve the effects of improving industrial production efficiency, reducing wrinkles, and ensuring activity
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Embodiment 1
[0041] The acquisition of embodiment 1 wild ginseng cell
[0042] 1. Preparation of culture medium
[0043] (1) Prepare wild ginseng callus induction medium, add 0.2mg / L-0.5mg / LGA (gibberellin), 20g / L-30g / L sucrose, 0.6g / L-0.8g / L agar.
[0044] (2) Prepare wild ginseng cell culture medium, add 0.2mg / L-0.5mg / L6-BA (6-benzylpurine), 2-4mg / L NAA (naphthaleneacetic acid ), 0.6g / L-0.8g / L agar and 20g / L-30g / L sucrose.
[0045] Table 1. MS basal medium
[0046]
[0047]
[0048] 2. Induction of wild ginseng cells
[0049] (1) Wild ginseng callus preparation: take wild wild ginseng roots as explants, remove the surface epidermis after disinfection, inoculate on callus induction medium, and culture at 25±2°C to obtain callus;
[0050] (2) Cultivation of wild ginseng cells: take the wild ginseng callus in step (1), inoculate it in the wild ginseng cell culture medium, cultivate it at 25±2°C, and transfer it once every 30-40 days;
[0051] (3) Subculture and screening of wil...
Embodiment 2
[0068] A preparation method of an anti-aging cream containing Changbai Mountain wild ginseng cell water extract, comprising the following steps:
[0069] (1) Add pure water into the water phase pot, turn on the rapid stirring, and put the acrylic acid (ester) / C 10-30 Alkyl acrylate cross-linked polymer, (butanediol, glycerin, xanthan gum, sodium hyaluronate and sodium polyglutamate, pre-mixed) slowly added and fully stirred to fully disperse and swell, add trehalose, allantoic Sodium, EDTA-2Na, dipotassium glycyrrhizinate, betaine, methylparaben, add 1.5% pure water;
[0070] (2) Heating up (target 85°C, deviation ±3°C), after the water phase pot is heated up to the target temperature, add the raw material hydrogenated lecithin, keep stirring for 10 minutes; at the same time, carry out the operation of the oil phase pot:
[0071] (3) PEG-20 Methyl Glucose Sesquistearate, Methyl Glucose Sesquistearate, Squalane, Jojoba Seed Oil, Caprylic / Capric Triglyceride, Dimethicone Add o...
Embodiment 3
[0084] A preparation method of an anti-aging cream containing Changbai Mountain wild ginseng cell water extract, comprising the following steps:
[0085](1) Add pure water to the water phase pot, start rapid stirring, add ammonium acryloyldimethyl taurate / VP copolymer, (butanediol, glycerin, xanthan gum, sodium hyaluronate and polyglutamic acid Sodium, pre-mixed) slowly added and fully stirred to fully disperse and swell, add trehalose, allantoin, EDTA-2Na, dipotassium glycyrrhizinate, betaine, methylparaben, and add 1.5% pure water;
[0086] (2) Heating up (target 85°C, deviation ±3°C), after the water phase pot is heated up to the target temperature, add the raw material hydrogenated lecithin, keep stirring for 10 minutes; at the same time, carry out the operation of the oil phase pot:
[0087] (3) Polysorbate-20, potassium cetyl phosphate, squalane, jojoba seed oil, caprylic / capric triglyceride, dimethicone, tocopheryl acetate, spermaceti Add stearyl alcohol and propylpara...
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