Method for improving nitrogen removal of heterotrophic nitrification-aerobic denitrification bacteria
An aerobic denitrifying bacteria and heterotrophic nitrification technology, applied in chemical instruments and methods, microorganism-based methods, biochemical equipment and methods, etc., can solve the problem of denitrification without heterotrophic nitrification-aerobic denitrifying bacteria. and other problems, to achieve the effect of improving the denitrification capacity of heterotrophic nitrification-aerobic denitrification bacteria, improving the denitrification effect, and improving the denitrification capacity.
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Embodiment 1
[0029] The separation of bacteriophage in the waste water of embodiment 1
[0030] (1) Preparation of sewage samples
[0031] The collected sewage was treated, centrifuged at 12000r / min at 4°C for 10min, and the centrifuged supernatant was filtered through a 0.22um filter membrane, and the filtrate was the treated sewage sample.
[0032] (2) Preparation of heterotrophic nitrification-hotrophic denitrification bacteria suspension
[0033] The heterotrophic nitrifying-eutrophic denitrifying bacteria DNF23 preserved in the laboratory (preserved in the Guangdong Provincial Microbial Culture Collection Center on July 5, 2019, and its preservation number is GDMCC No: 60713.) was inoculated by the three-section line method On the heterotrophic nitrification solid medium, culture in a biochemical incubator for two days, use a sterile loop to pick the strain and inoculate it in the heterotrophic nitrification liquid medium, and place it in a constant temperature shaker for overnight c...
Embodiment 2
[0037] The determination of embodiment 2 phage
[0038] (1) Determination of optimal multiplicity of infection
[0039] The bacteriophage PSA6 proliferation liquid obtained in Example 1 and the DNF23 bacterium liquid were inoculated into LB liquid medium at a ratio of MOI of 0.000001, 0.00001, 0.0001, 0.001, 0.01, 0.1, 1 and 10, and placed in a constant temperature shaker overnight to cultivate. The next day, the culture solution was taken, centrifuged at 12000 r / min at 4°C for 10 minutes, the supernatant was taken and filtered through a 0.22um filter membrane, and the filtrate was tested for phage PSA6 titer by the double-layer plate method.
[0040] The optimal multiplicity of infection refers to the ratio of the number of phages to the number of host bacteria before infection occurs, and the MOI that produces the highest phage titer is determined as the optimal multiplicity of infection. Results It can be seen from Table 1 that the optimal multiplicity of infection of pha...
Embodiment 3
[0048] Example 3 Fe 3+ Assay for Inhibition of Phage Proliferation
[0049] Using ferric chloride, the preparation contains different concentrations of Fe 3+ (0, 100mg / L and 300mg / L) LB liquid culture medium, bacteriophage PSA6 and DNF23 bacterium are inoculated in the liquid medium with optimal multiplicity of infection. The culture solution was placed in a constant temperature shaker, and cultured at 25°C and 120r / min for 48h. At the same time, a blank group and parallel experiments were set up. The culture solution was taken every 12 hours from 0h, centrifuged at 12000r / min for 10min, filtered, and the titer of phage in the supernatant was measured.
[0050] The result is given by figure 2 It can be seen that with concentrations of 100mg / L and 300mg / L Fe 3+ Acting on phage PSA6, its titer dropped to 0 within 12h, indicating that Fe 3+ Has a removal effect on phages.
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