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Cyclic beta-1,2-glucan and curcumin inclusion compound and preparation method thereof

A technology of dextran and curcumin, applied in the biological field, can solve the problems of low water solubility, low bioavailability, and poor stability of curcumin, and achieve the effect of low bioavailability, simple preparation process, and good stability

Pending Publication Date: 2022-02-25
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, curcumin's low water solubility, low bioavailability, easy degradation, and poor stability limit its wide application in clinical practice.

Method used

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  • Cyclic beta-1,2-glucan and curcumin inclusion compound and preparation method thereof
  • Cyclic beta-1,2-glucan and curcumin inclusion compound and preparation method thereof
  • Cyclic beta-1,2-glucan and curcumin inclusion compound and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0092] Example 1: Preparation of cyclic β-1,2-glucan

[0093] Specific steps are as follows:

[0094] 1. Preparation of extracellular neutral oligosaccharides by shake flask fermentation of Rhizobium radiobacter ATCC 1333

[0095] (1) Inoculate Rhizobium radiobacter ATCC 1333 on the slant medium, and culture it upside down at 30°C. After culturing for 48-72 hours, pick 2-3 rings of single colonies and inoculate them into 50mL seed medium on the ultra-clean bench , 30°C, 200rpm conditional culture for 24h, the activated seed solution is obtained;

[0096] (2) Inoculate the seed liquid obtained in step (1) in the fermentation medium with an inoculum size of 10% (v / v), 30°C, 200rpm, ferment and cultivate for 144h, prepare a fermented liquid, and detect the fermented liquid in the fermented liquid The extracellular neutral oligosaccharides (the total sugar content in the fermentation broth minus the acid sugar content, roughly estimated as the neutral sugar content), its content...

Embodiment 2

[0104] Example 2: Purification of cyclic β-1,2-glucan

[0105] Specific steps are as follows:

[0106] Step 1: Concentrate the fermentation supernatant obtained in Example 1 by rotary evaporation, centrifuge at 10,000 rpm for 10 minutes, take the supernatant, add 3 times the volume of absolute ethanol to the supernatant, and incubate at 4°C for 12 hours Afterwards, centrifuge at 10000rpm for 10min, and get the supernatant;

[0107] The supernatant was concentrated to 1 / 10 of the original volume to obtain a concentrated solution; 3 times the volume of absolute ethanol was added to the concentrated solution so that the final concentration of ethanol reached 75% (v / v) to obtain a mixture, which was After the mixture was placed at 4° C. for 12 hours, it was centrifuged at 10,000 rpm for 10 minutes, the supernatant was collected, and the supernatant was concentrated to 1 / 5 of the original volume to obtain a concentrate.

[0108] Step 2: Continue to add ten times the volume of abs...

Embodiment 3

[0136] Example 3: Structural analysis of cyclic β-1,2-glucan

[0137] The neutral oligosaccharides obtained in Example 2 were subjected to structural analysis.

[0138] (1)MALDI-TOF-MS

[0139] Test conditions: The molecular weight of the purified neutral oligosaccharides was analyzed by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS). The specific method is as follows: 1 μL of oligosaccharide sample (1 g / L) is spotted on the target, and dried in a desiccator under negative pressure. Spot 1 μL of DHB matrix solution on the same position, and dry in a desiccator under negative pressure. The sample is measured after mixing and crystallizing with the matrix. Analysis was performed in reflector mode in the mass range m / z 2000-4500. The results showed that the degree of polymerization of neutral oligosaccharides was 17-23.

[0140] (2) Monosaccharide composition

[0141] Test conditions: The obtained neutral oligosaccharides were co...

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Abstract

The invention discloses a cyclic beta-1,2-glucan and curcumin inclusion compound and a preparation method thereof, and belongs to the technical field of biology. The invention provides preparation and characterization of cyclic beta-1, 2-glucan by a fermentation method as well as a preparation method and structural analysis of curcumin, and belongs to the technical field of biology. According to the invention, fermentation synthesis (two-stage pH fermentation) is carried out in a 7-L fermentation tank, and the yield reaches 2.79 g / L. Pure neutral oligosaccharide obtained through purification methods such as ethanol fractional alcohol precipitation, SPE solid-phase extraction, DEAE-Sepharose FF ion exchange column chromatography and the like is identified as the cyclobeta-1, 2-glucan which is composed of glucose monomers, has the polymerization degree range of 17-23 and mainly has the polymerization degree of 19. The inclusion compound is successfully formed by the ring beta-1,2-glucan and the curcumin, so that the solubility of the curcumin can be remarkably improved, and the bioavailability of the curcumin is improved.

Description

technical field [0001] The invention relates to an inclusion compound of cyclic β-1,2-glucan and curcumin and a preparation method thereof, belonging to the field of biotechnology. Background technique [0002] Cyclic β-1,2-glucans (Cyclicβ-1,2-glucans) refer to a class of cyclic polysaccharides composed of glucose monomers connected by β-1,2 glycosidic bonds, with a degree of polymerization distribution of 17 ~25, and the degree of polymerization can reach 40 in some strains. Cyclic β-1,2-glucan can be divided into unbranched structure and branched structure, and the substituents of branched structure include phosphoglyceride group, methylmalonyl group and succinyl group. And the degree of polymerization and substituents vary greatly among different bacterial species. Because the structures of cyclo-β-1,2-glucan and cyclodextrin are very similar, both are ring structures composed of glucose monomers. [0003] Curcumin (curcumin), its structural formula is as follows: I...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/02A23L33/105A23L29/30A23P10/30A23L2/52C12P19/08C12R1/41
CPCC08B37/0024C12P19/08A23L33/105A23L29/30A23P10/30A23L2/52A23V2002/00A23V2200/30A23V2250/2112A23V2250/5034
Inventor 詹晓北吴传超朱莉蒋芸张洪涛吴剑荣
Owner JIANGNAN UNIV
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