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Process for extracting picroside from rhizoma picrorhizae through fermentation

A technology of picroside and picroside, applied in the field of fermentative extraction of picroside, can solve the problems of low extraction efficiency of picroside total saponins, unstable process and the like, and achieve stable properties, strong systemicity and good reproducibility of separated products. Effect

Pending Publication Date: 2022-03-01
山东康裕生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, there are many reports in the literature on the method of extracting the total saponins of Rhizoma Rhizoma Pedis, most of which adopt ethanol reflux extraction and then cooperate with macroporous resin as the separation and preparation method of the adsorbent, but the extraction efficiency of total saponins of Rhizoma Rhizome is low, and the process is not good. Stablize

Method used

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  • Process for extracting picroside from rhizoma picrorhizae through fermentation

Examples

Experimental program
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Effect test

Embodiment 1

[0029] Such as figure 1 Shown, a kind of technology that extracts berberine from fermented berberine, comprises the following steps:

[0030] Step 1. Crush the raw material of Coptis chinensis: crush the raw material of Coptis chinensis to a particle size of 100-200 meshes, and set aside. rhizome;

[0031] Step 2, preparation of enzyme preparation: disperse cellulase, hemicellulase, protease, amylase, glycosidase in water to obtain enzyme preparation, the concentration of cellulase is 14u / g, and the concentration of hemicellulase is 20u / g, the concentration of protease is 2u / g, the concentration of amylase is 3u / g, and the concentration of glycosidase is 2u / g;

[0032] Step 3. Preparation of fermentation product: add sucrose ester and ammonium oxalate to the enzyme preparation, mix evenly, add crushed Rhizoma Peptides, stir at a low speed of 20°C for 8 hours at a stirring speed of 40r / min, and inactivate at a high temperature to obtain a fermentation product, enzyme prepara...

Embodiment 2

[0036] Such as figure 1 Shown, a kind of technology that extracts berberine from fermented berberine, comprises the following steps:

[0037] Step 1. Crush the raw material of Coptis chinensis: crush the raw material of Coptis chinensis to a particle size of 100-200 meshes, and set aside. rhizome;

[0038] Step 2, preparation of enzyme preparation: disperse cellulase, hemicellulase, protease, amylase, glycosidase in water to obtain enzyme preparation, the concentration of cellulase is 16u / g, and the concentration of hemicellulase is 23u / g, the concentration of protease is 3u / g, the concentration of amylase is 4u / g, and the concentration of glycosidase is 2.5u / g;

[0039] Step 3. Preparation of fermentation product: add sucrose ester and ammonium oxalate to the enzyme preparation, mix evenly, add crushed Rhizoma Peptides, stir at a low speed of 23°C for 9 hours at a stirring speed of 50r / min, and inactivate at a high temperature to obtain a fermentation product, enzyme prepara...

Embodiment 3

[0043] Such as figure 1 Shown, a kind of technology that extracts berberine from fermented berberine, comprises the following steps:

[0044] Step 1. Crush the raw material of Coptis chinensis: crush the raw material of Coptis chinensis to a particle size of 100-200 meshes, and set aside. rhizome;

[0045] Step 2, preparation of enzyme preparation: disperse cellulase, hemicellulase, protease, amylase, glycosidase in water to obtain enzyme preparation, the concentration of cellulase is 18u / g, and the concentration of hemicellulase is 26u / g, the concentration of protease is 4u / g, the concentration of amylase is 5u / g, and the concentration of glycosidase is 3u / g;

[0046] Step 3. Preparation of fermentation product: add sucrose ester and ammonium oxalate to the enzyme preparation, mix evenly, add crushed Rhizoma Peptides, stir at a low speed of 25°C for 10 hours at a stirring speed of 60r / min, and inactivate at a high temperature to obtain a fermentation product, enzyme prepar...

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Abstract

The invention discloses a process for fermenting and extracting picroside from rhizoma picrorhizae. The process comprises the following steps: crushing a rhizoma picrorhizae raw material; preparation of an enzyme preparation: dispersing cellulase, hemicellulase, protease, amylase and glycosidase in water to obtain the enzyme preparation; preparing a fermentation product: adding sucrose ester and ammonium oxalate into the enzyme preparation, uniformly mixing, adding the crushed rhizoma picrorhizae, and inactivating at high temperature; extracting a rhizoma picrorhizae extract: extracting a fermentation product by using an extraction solvent according to a percolation method, concentrating the extracted solution under reduced pressure, diluting, adsorbing by using purified macroporous adsorption resin, eluting, collecting eluent, concentrating under reduced pressure, drying and crushing; and obtaining picroside: dissolving the rhizoma picrorhizae extract with absolute ethyl alcohol, filtering, mixing filtrate into chromatographic silica gel for sample mixing, drying, eluting, and crushing to obtain the picroside. The process for extracting picroside from picrorhiza rhizome through fermentation is simple in preparation, high in picroside content, safe, easy to operate and low in cost.

Description

technical field [0001] The invention relates to the technical field of berberine extraction, in particular to a process for fermenting and extracting berberine from berberine. Background technique [0002] Huhuanglian is a perennial herb of Scrophulariaceae. It has a similar name to Coptis chinensis. Both of them are bitter cold, clearing away heat and dampness. They are good at removing dampness and heat in the stomach and intestines. At present, it has been found that the chemical components of Coptis chinensis mainly include iridoids, cucurbitacins, phenylethyl alcohol glycosides and phenolic glycosides, and also contain very small amounts of mannitol, berberine alcohol, berberine sterol, vanilla ethyl ketone and Aromatic acid, etc., iridoid glycosides (total saponins of Huhuanglian) as the main active ingredients have various pharmacological activities such as anti-hepatitis B virus, liver protection, and enzyme reduction. The effects of liver, gallbladder, and insectic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H1/08C07H17/04
CPCC07H1/08C07H17/04
Inventor 沈彩芹刘刚刘培冉刘乃兵赵学峰田雪文杨志强张志东
Owner 山东康裕生物科技有限公司
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