Composite microbial inoculant capable of efficiently degrading kitchen waste and preparation method of composite microbial inoculant

A technology of compound bacterial agent and kitchen waste, which is applied in biochemical equipment and methods, chemical instruments and methods, and microorganism-based methods, etc. Quantification, high temperature degradation efficiency, and the effect of shortening the degradation time

Pending Publication Date: 2022-03-01
UNIV OF ELECTRONICS SCI & TECH OF CHINA ZHONGSHAN INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] When using biological methods to treat food waste, it is difficult to achieve the desired effect with a single strain

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Described a kind of high-efficiency degrading food waste composite bacterial agent and preparation method thereof:

[0026] 1. Bacteria culture

[0027] (1) Strain activation: Bacillus licheniformis suspension, Bacillus thermophagocytic bacteria suspension, Bacillus subtilis suspension, Bacillus blackstone suspension, Brevibacterium suspension, Aspergillus versicolor suspension , Paenibacillus polymyxa suspension, Saccharomyces cerevisiae varietal suspension, Streptomyces thermophagosus suspension, and Bacillus xylosyllysinus suspension were inoculated in test tubes containing sterilized liquid medium , placed in an incubator with a temperature of 55 °C and a relative humidity of 50% for 4 days to obtain activated bacteria.

[0028] (2) Seed fermentation liquid: take (1) the activated bacterial liquid and inoculate it into conical flasks containing the corresponding liquid culture medium of each strain, place it at a temperature of 55°C and a relative humidity of 50%, ...

Embodiment 2

[0034] Described a kind of high-efficiency degrading food waste composite bacterial agent and preparation method thereof:

[0035] 1. Bacteria culture

[0036](1) Strain activation: Bacillus licheniformis suspension, Bacillus thermophagocytic bacteria suspension, Bacillus subtilis suspension, Bacillus blackstone suspension, Brevibacterium suspension, Aspergillus versicolor suspension , Paenibacillus polymyxa suspension, Saccharomyces cerevisiae varietal suspension, Streptomyces thermophagosus suspension, and Bacillus xylosyllysinus suspension were inoculated in test tubes containing sterilized liquid medium , placed in an incubator with a temperature of 55 °C and a relative humidity of 50% for 4 days to obtain activated bacteria.

[0037] (2) Seed fermentation liquid: take (1) the activated bacterial liquid and inoculate it into conical flasks containing the corresponding liquid culture medium of each strain, place it at a temperature of 55°C and a relative humidity of 50%, a...

Embodiment 3

[0043] Described a kind of high-efficiency degrading food waste composite bacterial agent and preparation method thereof:

[0044] 1. Bacteria culture

[0045] (1) Strain activation: Bacillus licheniformis suspension, Bacillus thermophagocytic bacteria suspension, Bacillus subtilis suspension, Bacillus blackstone suspension, Brevibacterium suspension, Aspergillus versicolor suspension , Paenibacillus polymyxa suspension, Saccharomyces cerevisiae varietal suspension, Streptomyces thermophagosus suspension, and Bacillus xylosyllysinus suspension were inoculated in test tubes containing sterilized liquid medium , placed in an incubator with a temperature of 55 °C and a relative humidity of 50% for 4 days to obtain activated bacteria.

[0046] (2) Seed fermentation liquid: take (1) the activated bacterial liquid and inoculate it into conical flasks containing the corresponding liquid culture medium of each strain, place it at a temperature of 55°C and a relative humidity of 50%, ...

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Abstract

The invention discloses a complex microbial inoculant for efficiently degrading kitchen waste and a preparation method thereof, and is characterized in that the complex microbial inoculant is prepared from 20-30 parts of bacillus licheniformis suspension, 10-20 parts of bacillus amyloliquefaciens suspension, 10-20 parts of bacillus subtilis suspension, 10-15 parts of bacillus licheniformis suspension, 5-15 parts of brevibacterium suspension and 5-10 parts of aspergillus versicolor suspension. The preparation method comprises the following steps: activating 10-25 parts of paenibacillus polymyxa bacterial suspension, 5-10 parts of beer yeast wine variant bacterial suspension, 5-10 parts of streptomyces thermophilus bacterial suspension and 5-15 parts of lysinibacillus xylolyticus bacterial suspension, carrying out expanded liquid culture, preparing concentrated bacterial suspension by a centrifugation method, adding the bacterial suspension of each strain according to a certain ratio, carrying out fermentation, and carrying out freeze-drying to obtain the microbial inoculant containing the paenibacillus polymyxa, the beer yeast wine variant bacterial suspension, the streptomyces thermophilus and the lysinibacillus xylolyticus. A high-efficiency bacterial strain composite system is constructed, and the high-efficiency bacterial strain composite system is uniformly mixed with a carrier material composed of sawdust, bran and activated carbon in a certain mass ratio, so that the composite microbial preparation is prepared, and high-efficiency degradation of the kitchen waste is realized.

Description

technical field [0001] The invention relates to the field of kitchen waste treatment, in particular to a bacterial agent for degrading kitchen waste and a preparation method thereof. Background technique [0002] Kitchen waste generally refers to daily cooking residues and discarded meals. It is rich in organic matter such as starch, cellulose, protein, and lipid. Improper disposal will breed pathogenic bacteria, produce stench, and seriously pollute the environment. At present, the treatment methods of food waste are mainly divided into non-biological technology and biological technology to deal with kitchen waste. Non-biological treatment technologies include incineration, landfill, and vacuum frying, etc.; biological treatment technologies mainly include vermicomposting, microbial Methods such as composting and anaerobic fermentation, among which aerobic composting technology and anaerobic fermentation technology are the two most widely used treatment technologies so far....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16C12N1/14B09B3/60B09B101/70C12R1/10C12R1/07C12R1/125C12R1/13C12R1/12C12R1/865C12R1/465C12R1/66
CPCC12N1/20C12N1/16C12N1/14B09B3/00
Inventor 王悦辉施力仁林凯文施曼云钟建军
Owner UNIV OF ELECTRONICS SCI & TECH OF CHINA ZHONGSHAN INST
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