Purification preparation method of sulfopeptide indicator
An indicator and sample technology, which is applied in the field of purification and preparation of sulfopeptide indicators, can solve the problems of the influence of seawater pH value and total alkalinity on the accurate measurement, the cumbersome operation of the indicator purification method, and the limitation of the wide application of photometric method. Low cost, easy operation, good purification and preparation effect
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Embodiment 1
[0025] The m-cresyl violet indicator is purified and prepared by using the present invention.
[0026] Weigh 98.07 mg of a certain brand of m-cresyl violet indicator into a sample bottle, add 4 mL of N,N-dimethylformamide, mix well, let stand for 5 minutes, ultrasonicate for 10 minutes, pass through a 0.22 μm filter membrane and place in in the syringe. Inject the sample in the syringe into the fast liquid phase preparative chromatograph for detection and separation. The organic phase in the mobile phase is acetonitrile, and the inorganic phase is ultrapure water. The gradient elution procedure is used. The procedure is shown in Table 1, and the flow rate is 40mL / min . The separation column used for analysis and detection is SΛNTΛITECHNOLOGIES SEPA Flash spherical C18 rapid separation column, the specifications are: column size 90g, particle size 40-60μm, pore size The detection wavelength is 434nm. Collect the purified samples separated by the rapid preparative liquid chr...
Embodiment 2
[0031] Purify and prepare the phenol red indicator by using the present invention.
[0032]Weigh 48.28mg of a certain brand of phenol red indicator into a sample bottle, add 1mL of N,N-dimethylformamide, mix well, let stand for 5min, sonicate for 10min, pass through a 0.22μm filter membrane and place in a centrifuge tube middle. Inject the sample in the syringe into the fast liquid phase preparative chromatograph for detection and separation. The organic phase in the mobile phase is acetonitrile and the inorganic phase is ultrapure water. The gradient elution program is used. The program is shown in Table 2, and the flow rate is 40mL / min . The separation column used for analysis and detection is SΛNTΛITECHNOLOGIES SEPA Flash spherical C18 rapid separation column. The model specifications are: column size 90g, particle size 40-60μm, pore size The detection wavelength is 400nm. Collect the purified samples separated by rapid preparative liquid chromatography at 33 to 36 minu...
Embodiment 3
[0037] This embodiment includes the following steps:
[0038] 1) Weigh 40 mg of thymol blue and place it in a sample bottle, add 1 to 10 mL of N,N-dimethylformamide, mix well, let stand for 5 minutes, and ultrasonicate for 5 minutes; the prepared concentration of thymol blue is 50 ~100mmol / L;
[0039] 2) Take the sample in step 1) and filter it through a 0.22 μm filter membrane and place it in a syringe;
[0040] 3) Inject the sample in the syringe of step 2) into a fast liquid phase preparative chromatograph for detection and separation; when detecting in the fast liquid phase preparative chromatograph, the organic phase in the mobile phase is acetonitrile, and the inorganic phase is ultrapure water, Gradient elution procedure is adopted, the proportion of organic phase is 5%-90%; the chromatographic column used for separation is C18 rapid separation column, the particle size is 40-60 μm, and the pore size is When performing fast liquid phase preparative chromatographic de...
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Abstract
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