Nanogold biochip for detecting Alzheimer's disease marker as well as preparation method and application of nanogold biochip

An Alzheimer's disease, biochip technology, applied in measurement devices, material excitation analysis, fluorescence/phosphorescence, etc., can solve the problems of not reaching the picogram level, inaccurate results, low sensitivity, etc., and improve the emission intensity. , the effect of improving the detection signal and improving the sensitivity

Pending Publication Date: 2022-03-01
SHENZHEN UNIV +1
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among these research methods, the results of the colorimetric method are intuitively visible to the naked eye, but the sensitivity is low; although the sensitivity of the fluorescence method is slightly higher than that of the colorimetric method, it still cannot reach the picogram level, and it is susc

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Nanogold biochip for detecting Alzheimer's disease marker as well as preparation method and application of nanogold biochip
  • Nanogold biochip for detecting Alzheimer's disease marker as well as preparation method and application of nanogold biochip
  • Nanogold biochip for detecting Alzheimer's disease marker as well as preparation method and application of nanogold biochip

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0079] Example 1

[0080] Preparation and protein testing of Aβ40 nano gold biocontrol

[0081] 1, preparation of nano-gold biocontrol

[0082] (1) Golden nanoparticles by wet method on the glass substrate to obtain a nano-gold chip;

[0083] (2) Print the capture antibody of a concentration of 1.0 μg / ml of the concentration of 1.0 μg / ml on the nano-gold chip by using a micro-point gauge.

[0084] (3) Further print chicken IGY antibody on the chip is a nano-gold biocontrol;

[0085] 2, capture antibody and fluorescent molecule Irdye800 coupling

[0086] (4) The captured antibody of the Aβ40 and the fluorescent molecule IrDye 800 were mixed according to the molar ratio of 1:10, the reaction was half an hour, and then mixed uniform, and the reaction was continued for half an hour;

[0087] (5) After the reaction is completed, the amount of PBS is added to make the reaction system volume reaches 500 μl;

[0088] (6) Add NAP-5 column with 10 mL of PBS, wash the column. When the so...

Example Embodiment

[0101] Example 2

[0102] Preparation and protein detection of Aβ42 nano gold biocontrol

[0103] Preparation of A [beta] 42 nano-gold biocontrol and preparation method of the Aβ40 nano gold biocontrol in Example 1 was to print the capture antibody of Aβ42 in the nano-gold chip to obtain a Aβ42 nano-gold biocontrol;

[0104] A [beta] 42 protein detection of Aβ42 protein was detected in Example 1 was a standard curve of Aβ42 by Aβ42 protein standard, and the protein to be tested was Aβ42 protein.

[0105] The fluorescent image of the Aβ42 and the standard curve such as Figure 5 Disted, where Y = 0.55x + 0.34, R 2 = 0.994.

[0106] In summary, the present invention proposes a method for detecting a nano-gold biocontrol of Alzheimer's markers, and the gold nanoparticles are deposited on the chip substrate, so that the chip surface distributes many discontinuous, Golden nanoparticles with uniform spacing, and control the size of the gold nanoparticles at 20-50 nm, the spacing is contr...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Diameteraaaaaaaaaa
Login to view more

Abstract

The invention provides a nanogold biochip for detecting an Alzheimer's disease marker and a preparation method and application of the nanogold biochip. Gold nanoparticles are deposited on a chip substrate, so that a plurality of discontinuous gold nanoparticles with uniform intervals are distributed on the surface of the chip; and fixing a capture antibody for detecting the Alzheimer's disease marker on the chip through printing to form a capture layer, thereby obtaining the nanogold biochip. A local electric field is generated due to gaps of the gold nanoparticles, so that the emission intensity of the coupled fluorescent molecules is improved, surface plasmas and the fluorescent molecules resonate, and fluorescence detection signal amplification is realized. The gold nanoparticles can also increase the specific surface area and increase binding sites for capturing the antibody. The near-infrared fluorescent molecules are adopted, so that background fluorescent signals of the substrate can be effectively avoided, and the signal-to-noise ratio is increased. Finally, background noise is reduced by detecting near-infrared fluorescence, a fluorescence output signal is enhanced by the gold nanoparticles, and the sensitivity of Alzheimer's disease biomarker detection is improved.

Description

technical field [0001] The invention relates to the field of biochemical detection, in particular to a nano-gold biochip for detecting Alzheimer's disease markers, a preparation method and application thereof. Background technique [0002] Alzheimer's disease (AD) is a complex neurodegenerative disease with a very long duration. The detection of blood markers is of great significance for the early diagnosis and universal screening of AD. However, the content of AD blood markers is low, which has exceeded the detection range of traditional detection methods (ELISA). Therefore, it is necessary to develop highly sensitive and specific detection methods to achieve ultra-sensitive detection of AD blood markers. [0003] Researchers have established colorimetric methods, fluorescence methods, electrochemical methods, electrochemiluminescence methods and other methods for detection. Among these research methods, the results of the colorimetric method are intuitively visible to the...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N21/64G01N21/01
CPCG01N21/6486G01N21/01
Inventor 徐庆刘一镳刘琼王国新廖滔陈敏文
Owner SHENZHEN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap