Organic-inorganic hybrid gold nanoparticles based on II-type collagen targeting peptide as well as preparation method and application of organic-inorganic hybrid gold nanoparticles

Abstract

The invention discloses an organic-inorganic hybrid gold nanoparticle based on type II collagen targeting peptide and a preparation method and application thereof, the core of the nanoparticle is a gold nanoparticle, the surface of the gold nanoparticle is coated with a compact polydopamine coating layer, and the surface of the polydopamine is grafted with the type II collagen targeting peptide. The combination of the nano-particles and the articular cartilage matrix prevents the nano-particles from being quickly cleared away from bone joint synovial fluid, the free gold nano-particles which are not combined with the articular cartilage matrix are quickly cleared away, and the numbers of the gold nano-particles reserved in normal and degenerated cartilage are different, so that different photoacoustic imaging intensities are caused; the kit can be used for early diagnosis of osteoarthritis lesion. The quinonyl group on the polydopamine on the surface of the nanoparticle can effectively remove wide free radicals, inhibit the expression level of cell active oxygen and active nitrogen in cartilage stromal cells and promote the activity of antioxidant enzyme, so that excellent effects of treating and relieving osteoarthritis and protecting cartilage are achieved.

Description

technical field [0001] The invention belongs to the technical field of nanobiological materials, and in particular relates to an organic-inorganic hybrid gold nanoparticle based on type II collagen targeting peptide, a preparation method and application thereof. Background technique [0002] Osteoarthritis affects approximately 237 million people worldwide with the increasing risk of disability associated with exercise and activity in recent years. Articular cartilage contains chondrocytes, proteoglycans and collagen, and covers the surface of bone joints. It is a complex water-containing tissue that provides a smooth contact surface for bone joints to reduce friction during joint movement. and vibration. The degeneration of articular cartilage is the main feature and main pathological change in the early stage of osteoarthritis and throughout the course of the disease. Timely and accurate diagnosis in the early stage of osteoarthritis is the key to the treatment of osteoa...

AI Technical Summary

Problems solved by technology

[0007] When the small-sized photoacoustic imaging nanomaterial probe with strong near-infrared light absorption ability smoothly penetrates the dense type II collagen network on the surface of the articular cartilage matrix and enters the matrix, the free nanoparticles will be quickly removed by the synovial fluid in the matrix. It is impossible to detect and diagnose the occurrence of osteoarthritis, which is also one of the problems that need to be solved

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