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Antibacterial peptide MAMP-03 and application thereof

A technology of MAMP-03 and antimicrobial peptides, which is applied in antibacterial drugs, peptides, preparations for toiletry, etc., can solve the problems of limited effect, large cationic antibacterial peptides, and toxic effects, and achieve good application prospects and good antibacterial active effect

Pending Publication Date: 2022-03-08
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, antimicrobial peptides still face many unresolved problems as antibacterial drugs: some cationic antimicrobial peptides can produce greater toxicity to mammalian cells; the amphipathic structure requires a certain number of basic amino acids in the amino acid sequence, and the The property that permanent amino acids are easily hydrolyzed by proteases greatly limits their effects as drugs after they enter the human body

Method used

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  • Antibacterial peptide MAMP-03 and application thereof
  • Antibacterial peptide MAMP-03 and application thereof
  • Antibacterial peptide MAMP-03 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Preparation and identification of hydrolyzed milk protein peptides.

[0014] (1) Sample preparation

[0015] Take 1g of cow casein and dissolve it in 10ml of water, add 20mg of trypsin and react at 37°C for 4 hours. After the enzymolysis process, centrifuge at 14000×g and 4°C for 20min, and the supernatant is the hydrolyzed milk protein peptide sample.

[0016] (2) LC-MS / MS analysis

[0017] The hydrolyzed milk protein peptide solution sample was desalted with a C18 column (Waters Oasis HLB SPE column, 1cc / 30mg, 30um) according to the following steps: the C18 column was activated with 1.5mL methanol and added 1.5mL 0.1% (V / V) TFA (trifluoro Acetic acid)-H2O solution balance, the sample was redissolved with 1ml 0.1% (V / V) TFA-H2O solution and added to the C18 column, and 1.5mL 80% (V / V) ACN (acetonitrile) / 0.1% (V / V) TFA-H2O solution was used for elution, and the eluate was collected, subpackaged, freeze-dried and stored at -80°C. The desalted sample was dissolved in ...

Embodiment 2

[0032] Antibacterial activity of peptides.

[0033] The peptide MAMP-03 was chemically synthesized in Shanghai Jiepeptide Technology Co., Ltd. with a purity of 97.6%. Escherichia coli and Porphyromonas gingivalis in Gram-negative bacteria, Staphylococcus aureus and Streptococcus mutans in Gram-positive bacteria were used as target strains to investigate the antibacterial activity of polypeptide MAMP-03.

[0034] (1) Strains and recovery

[0035] Escherichia coli (Escherichia coli K12), Staphylococcus aureus (Staphylococcus aureus), Porphyromonas gingivalis (P.g., ATCC33277) and Streptococcus mutans (M.s., ATCC25175) were purchased from Chinese strain resources Library preservation center, stored at -80°C with glycerol preservation method. Before the experiment, Escherichia coli was inoculated in LB broth medium (Beijing Suo Lai Bao Technology Co., Ltd.), Staphylococcus aureus was inoculated in TSB broth medium (Beijing Suo Lai Bao Technology Co., Ltd.), and gingival porphyri...

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Abstract

The invention relates to a polypeptide compound MAMP-03 which is derived from hydrolyzed milk protein and has microbial inhibition activity. The amino acid sequence of the polypeptide compound MAMP-03 is Leu-Asp-Thr-Asp-Tyr-Lys-Lys-Tyr (LDTDYKKY. The polypeptide MAMP-03 has wide inhibitory activity on gram-positive bacteria and gram-negative bacteria, including escherichia coli, porphyromonas gingivalis, streptococcus mutans and the like. The compound has the advantages of natural and safe raw materials and low production cost, and can be applied to medicines, foods and daily necessities as an active component for inhibiting microbial growth.

Description

technical field [0001] The invention relates to an antimicrobial peptide MAMP-03 (LDTDYKKY) derived from hydrolyzed milk protein and its application. Background technique [0002] Antibiotics are drugs that can resist pathogenic microorganisms and are the largest category of antibacterial and anti-inflammatory drugs. Antibiotics are substances produced by bacteria, fungi, or other microorganisms in the course of life, and can inhibit or kill bacteria, fungi, and other pathogenic microorganisms. Therefore, they are widely used in various infectious diseases as anti-infective drugs. The remarkable effect of antibiotics in treating infections has also led to bacterial resistance, making the treatment of some infectious diseases increasingly difficult. As a new type of antibiotic, antimicrobial peptides have broad-spectrum antibacterial activity and will not easily make microorganisms resistant to drugs, so they have broad application prospects. [0003] Antimicrobial peptides...

Claims

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Application Information

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IPC IPC(8): C07K7/06A61K8/64A61K38/08A61P1/02A61P17/00A61P31/04A61Q17/00
CPCC07K7/06A61K38/08A61P31/04A61P1/02A61P17/00A61K8/64A61Q17/005Y02A50/30
Inventor 王方军靳艳杨建
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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