Unlock instant, AI-driven research and patent intelligence for your innovation.

Bionic hydrogel for regulating and controlling bone and cartilage cells to promote repair and preparation method thereof

A chondrocyte and hydrogel technology, applied in tissue regeneration, medical science, prosthesis, etc., can solve problems such as lack of research, and achieve the effect of promoting proliferation and differentiation, promoting bone growth, and promoting regeneration and repair

Pending Publication Date: 2022-03-08
SOUTHWEST JIAOTONG UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The main purpose of the present invention is to provide a biomimetic double-layer hydrogel preparation method that regulates bone and chondrocytes to promote repair, so as to solve the relatively lack of research on adding two therapeutic ions to biomimetic hydrogel scaffolds in the prior art question

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Bionic hydrogel for regulating and controlling bone and cartilage cells to promote repair and preparation method thereof
  • Bionic hydrogel for regulating and controlling bone and cartilage cells to promote repair and preparation method thereof
  • Bionic hydrogel for regulating and controlling bone and cartilage cells to promote repair and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0036] A method for preparing a biomimetic hydrogel that regulates bone and chondrocytes to promote repair, comprising the following steps:

[0037]Step 1, the polyglutamic acid of 5-9 weight part, the carboxymethyl chitosan of 4-6 weight part, the bacterial cellulose of 5-9 weight part and the copper sulfate of 0.05-0.09 weight part are dissolved in 80- 150 parts by weight of ultrapure water, mixed for later use;

[0038] Step 2: the polyglutamic acid of 5-9 weight part, the carboxymethyl chitosan of 4-6 weight part, the bacterial cellulose of 5-9 weight part and the magnesium sulfate of 1-3 weight part are dissolved in 80- 150 parts by weight of ultrapure water, mixed for later use;

[0039] Step 3. Add 1-2 parts of N-hydroxysuccinimide and 2-3 parts of 1-(3-dimethylaminopropyl)-3 ethylcarbodiimide salt to the mixed solution obtained in step 1 After the acid salt is stirred evenly, pour it into a grinding tool to form a bone layer, add 1-2 parts of N-hydroxysuccinimide, 2-...

Embodiment 1

[0047] Step 1, the polyglutamic acid of 5, the carboxymethyl chitosan of 4, the bacterial cellulose of 5 and the copper sulfate of 0.05g are dissolved in the ultrapure water of 80g, mix stand-by;

[0048] Step 2: dissolve the polyglutamic acid of 5g, the carboxymethyl chitosan of 4g, the bacterial cellulose of 5g and the magnesium sulfate of 1g in the ultrapure water of 80g, mix and set aside;

[0049] Step 3. Add 1 part of N-hydroxysuccinimide and 2 parts of 1-(3-dimethylaminopropyl)-3 ethylcarbodiimide hydrochloride to the mixed solution obtained in step 1 and stir for 3 Pour into the mold after 2 minutes, continue to stand still for 3-6 minutes to form bone layer 2, add 1 part of N-hydroxysuccinimide, 2 parts of 1-(3-dimethylaminopropyl Base)-3 ethylcarbodiimide hydrochloride, pour it on the upper surface of the bone layer 2 after 3 minutes and continue to stand for 3-6 minutes to form the cartilage layer 1, and finally obtain the biomimetic hydrogel that regulates bone and...

Embodiment 2

[0051] Step 1, the polyglutamic acid of 7g, the carboxymethyl chitosan of 5g, the bacterial cellulose of 7g and the cupric sulfate of 0.07g are dissolved in the ultrapure water of 110g, mix stand-by;

[0052] Step 2: dissolve the polyglutamic acid of 7g, the carboxymethyl chitosan of 5g, the bacterial cellulose of 7g and the magnesium sulfate of 2g in the ultrapure water of 110g, mix and set aside;

[0053] Step 3. Add 1.5 parts of N-hydroxysuccinimide and 2.5 parts of 1-(3-dimethylaminopropyl)-3 ethylcarbodiimide hydrochloride to the mixed solution obtained in step 1 and stir for 3 Pour into the mold after 2 minutes, continue to stand still for 3-6 minutes to form bone layer 2, add 1.5 parts of N-hydroxysuccinimide, 2.5 parts of 1-(3-dimethylaminopropyl Base)-3 ethylcarbodiimide hydrochloride, pour it on the upper surface of the bone layer 2 after 3 minutes and continue to stand for 3-6 minutes to form the cartilage layer 1, and finally obtain the biomimetic hydrogel that reg...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses bionic hydrogel for regulating and controlling bone and cartilage cells to promote repair. The bionic hydrogel comprises an upper cartilage layer and a lower bone layer, and the cartilage layer comprises polyglutamic acid, carboxymethyl chitosan, bacterial cellulose, magnesium sulfate, water, N-hydroxysuccinimide and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride; the bone layer is prepared from polyglutamic acid, carboxymethyl chitosan, bacterial cellulose, copper sulfate, water, N-hydroxysuccinimide and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride; interfaces of the cartilage layer and the bone layer are tightly connected. The invention also discloses a preparation method of the bionic hydrogel for regulating and controlling bone and cartilage cells to promote repair.

Description

technical field [0001] The invention relates to the fields of biomedical materials, tissue engineering and medicine, in particular to a bionic hydrogel that regulates bone and cartilage cells to promote repair and a preparation method thereof. Background technique [0002] Articular cartilage is a kind of connective tissue in the skeletal system. Cartilage has no blood vessels. The biological activity and metabolism of mature cartilage are limited, and the ability to regenerate and repair is limited. When the defect of articular cartilage is not treated in time, it will deteriorate and lead to arthritis. Severe cases will lead to disability . In recent years, the therapeutic strategy of tissue engineering has made some progress. It has been reported in the literature that the proliferation and differentiation of bone marrow mesenchymal stem cells can be induced by embedding growth factors, which has a positive effect on osteochondral regeneration and repair. However, the li...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C08J3/075C08L77/04C08L1/02C08L5/08C08K3/30C08K5/29C08K5/3415C08K13/02A61L27/02A61L27/18A61L27/20A61L27/52A61L27/58
CPCC08J3/075A61L27/20A61L27/18A61L27/025A61L27/52A61L27/58C08J2377/04C08J2301/02C08J2477/04C08J2401/02C08J2405/08C08K2003/3063C08K2003/3045C08K5/3415C08K5/29C08K13/02A61L2300/102A61L2300/412A61L2300/404A61L2430/06A61L2430/02C08L5/08C08L1/02C08L77/04
Inventor 汪建新罗敏月江金波白家繁陈明霞何思源张航桢曾一力刘育豪
Owner SOUTHWEST JIAOTONG UNIV
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com