Bifidobacterium lactis SF-B21 and application of Bifidobacterium lactis SF-B21 in preparation of fermented beverage for increasing bone mineral density
A technology of SF-B21 and Bifidobacterium lactis, which is applied in the field of Bifidobacterium lactis SF-B21 and its preparation of bone density-increasing fermented drinks, can solve the side effects of patients, increase bone density and other problems, and achieve bone density improvement, high-viscosity Adhesion, prevention and treatment of osteoporosis
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[0050] The preparation method of each substance extract used in the present invention is: Poria cocos extract, eucommia leaf extract, astragalus extract, Codonopsis pilosula extract, licorice extract, wolfberry extract, and mulberry extract. The preparation process is as follows: after the raw materials are washed, Break into coarse grains, decoct twice with 10 times the amount of water for 3 hours and 1 hour, combine the two filtrates, let stand for 2 hours, and then filter to obtain the extract. The extract is concentrated to 1.10-1.15 (70°C) at a vacuum degree of -0.06 to 0.08Mpa, then spray-dried, and finally crushed into 80-mesh fine powder to obtain the extract.
[0051] The preparation method of each material powder is:
[0052] Walnut kernel powder, walnut kernels are dried to a water content below 5%, and ground into 80 mesh walnut kernel powder.
[0053] For yam powder, choose fresh yams that are smooth, free of disease spots, and straight and straight; remove the m...
Embodiment 1
[0059] Example 1 Isolation and identification of Bifidobacterium lactis SF-B21
[0060] 1. Bacterial source: yogurt; the collection time is January 2021, and the collection location is Xining City, Qinghai Province.
[0061] 2. Isolation of strains
[0062] Do 10-fold gradient dilution of the yogurt sample, take 100 μL of each gradient and evenly spread it on the MRS solid plate medium, then take it out and place it in an anaerobic tank, and culture it overnight at 37°C;
[0063] On the next day, colonies of various shapes and sizes could be seen growing on the plate, and some characteristic colonies of lactic acid bacteria were picked for second-generation streak purification, and the plate was anaerobically activated for 1 day to obtain cultures, which were then identified for strain identification.
[0064] 3. Identification results
[0065] The strain was identified as Bifidobacterium (Bifidobacterium genus), presumed to be Bifidobacterium animalis subsp. lactis (Bifidob...
Embodiment 2
[0066] Example 2 A fermented beverage for increasing bone density fermented with Bifidobacterium lactis SF-B21
[0067] Raw materials for preparing a fermented drink for increasing bone density, including a fermented substrate and a seasoning, wherein the fermented substrate includes the following components by weight:
[0068] 64 parts of purified water, 1 part of Poria cocos extract, 1 part of Eucommia leaf extract, 1 part of Astragalus extract, 1 part of Codonopsis pilosula extract, 2 parts of licorice extract, 2 parts of wolfberry extract, 2 parts of mulberry extract, walnut kernel 3 parts of yam powder, 3 parts of yam powder, 3 parts of red date powder, 3 parts of black sesame powder, 2 parts of oyster powder.
[0069] The seasoning comprises the following components by weight:
[0070] 1 part honey, 4 parts apple juice concentrate, 4 parts pear juice concentrate, 4 parts peach juice concentrate.
[0071] Prepare the fermented drink for increasing bone density according...
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